LOCUS pRBF 6850 bp ds-DNA circular 12-JUN-2008
DEFINITION .
ACCESSION .
VERSION .
SOURCE .
ORGANISM . .
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_feature 1724..1739
/label=rev_primer
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1..41
/label=Multiple cloning region
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1742..3526
/label=FLuc-fusion
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_feature 1874..3526
/label=FLuc
/ApEinfo_fwdcolor=#00c000
/ApEinfo_revcolor=#00c000
misc_feature 3558..3779
/label=SV40 Late poly(A) signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 146..195
/label=SV40 Promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3799..4035
/label=Enhancer
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature join(48..145,196..249)
/label=SV40 Promoter(1)
/ApEinfo_label=SV40 Promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 146..195
/label=SV40 transcription start sites region
/ApEinfo_fwdcolor=#ffb1be
/ApEinfo_revcolor=#ffb1be
misc_feature 519..1454
/label=RLuc
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_feature 276..408
/label=chimeric intron
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 453..471
/label=T7 promoter
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 470..470
/label=T7 transcription start +1
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 146..146
/label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1855..1871
/label=rev_primer(1)
/ApEinfo_label=rev_primer
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 184..184
/label=SV40 transcription start (+1)(1)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#fe00ff
/ApEinfo_revcolor=#fe00ff
misc_feature 1724..1739
/label=rev_primer(2)
/ApEinfo_label=rev_primer
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 190..190
/label=SV40 transcription start (+1)(2)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1506..1871
/label=IRES_of_p36
/ApEinfo_fwdcolor=#00bbff
/ApEinfo_revcolor=#00bbff
misc_feature 195..195
/label=SV40 transcription start (+1)(3)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1501..1526
/label=fwd_primer
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
ORIGIN
1 GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTGC ATCTCAATTA
61 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC
121 CGCCCATTCT CCGCCCCATC GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC
181 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
241 CAAAAAGCTG AAGTTGGTCG TGAGGCACTG GGCAGGTAAG TATCAAGGTT ACAAGACAGG
301 TTTAAGGAGA CCAATAGAAA CTGGGCTTGT CGAGACAGAG AAGACTCTTG CGTTTCTGAT
361 AGGCACCTAT TGGTCTTACT GACATCCACT TTGCCTTTCT CTCCACAGGT GTCCACTCCC
421 AGTTCAATTA CAGCTCTTAA GGCTAGAGTA CTTAATACGA CTCACTATAG GCTAGGCTTG
481 GCATTCCGGT ACTGAATTGA TATCAAGCTC TAGCCACCAT GACTTCGAAA GTTTATGATC
541 CAGAACAAAG GAAACGGATG ATAACTGGTC CGCAGTGGTG GGCCAGATGT AAACAAATGA
601 ATGTTCTTGA TTCATTTATT AATTATTATG ATTCAGAAAA ACATGCAGAA AATGCTGTTA
661 TTTTTTTACA TGGTAACGCG GCCTCTTCTT ATTTATGGCG ACATGTTGTG CCACATATTG
721 AGCCAGTAGC GCGGTGTATT ATACCAGACC TTATTGGTAT GGGCAAATCA GGCAAATCTG
781 GTAATGGTTC TTATAGGTTA CTTGATCATT ACAAATATCT TACTGCATGG TTTGAACTTC
841 TTAATTTACC AAAGAAGATC ATTTTTGTCG GCCATGATTG GGGTGCTTGT TTGGCATTTC
901 ATTATAGCTA TGAGCATCAA GATAAGATCA AAGCAATAGT TCACGCTGAA AGTGTAGTAG
961 ATGTGATTGA ATCATGGGAT GAATGGCCTG ATATTGAAGA AGATATTGCG TTGATCAAAT
1021 CTGAAGAAGG AGAAAAAATG GTTTTGGAGA ATAACTTCTT CGTGGAAACC ATGTTGCCAT
1081 CAAAAATCAT GAGAAAGTTA GAACCAGAAG AATTTGCAGC ATATCTTGAA CCATTCAAAG
1141 AGAAAGGTGA AGTTCGTCGT CCAACATTAT CATGGCCTCG TGAAATCCCG TTAGTAAAAG
1201 GTGGTAAACC TGACGTTGTA CAAATTGTTA GGAATTATAA TGCTTATCTA CGTGCAAGTG
1261 ATGATTTACC AAAAATGTTT ATTGAATCGG ACCCAGGATT CTTTTCCAAT GCTATTGTTG
1321 AAGGTGCCAA GAAGTTTCCT AATACTGAAT TTGTCAAAGT AAAAGGTCTT CATTTTTCGC
1381 AAGAAGATGC ACCTGATGAA ATGGGAAAAT ATATCAAATC GTTCGTTGAG CGAGTTCTCA
1441 AAAATGAACA ATAATTCTAG AGCTTATCGA TACCGTCGAC CTCGAATCAC TAGTCAGCTG
1501 gaattctggg cggtcaacaa gtgcgggcct ggctcagcgc gggggggcgc ggagaccgcg
1561 aggcgaccgg gagcggctgg gttcccggct gcgcgccctt cggccaggcc gggagccgcg
1621 ccagtcggag cccccggccc agcgtggtcc gcctccctct gggcgtccac ctgcccggag
1681 tactgccagc gggcatgacc gacccaccag gggcgccgcc gccggcgctc gcaggccgcg
1741 gatgaagaag aaaacccggc gccgctcgac ccggagcgag gagttgaccc ggagcgagga
1801 gttgaccctg agtgaggaag cgacctggag tgaagaggcg acccagagtg aggaggcgac
1861 ccagggcgaa gccATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC CATTCTATCC
1921 GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT ACGCCCTGGT
1981 TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT ACGCTGAGTA
2041 CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA
2101 CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT
2161 ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG AATTGCTCAA
2221 CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAAGGGGT TGCAAAAAAT
2281 TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG ATTCTAAAAC
2341 GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT
2401 TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGAC AAGACAATTG CACTGATCAT
2461 GAACTCCTCT GGATCTACTG GTCTGCCTAA AGGTGTCGCT CTGCCTCATA GAACTGCCTG
2521 CGTGAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT CAAATCATTC CGGATACTGC
2581 GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT
2641 GATATGTGGA TTTCGAGTCG TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTCTGAGGAG
2701 CCTTCAGGAT TACAAGATTC AAAGTGCGCT GCTGGTGCCA ACCCTATTCT CCTTCTTCGC
2761 CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGTGG
2821 CGCTCCCCTC TCTAAGGAAG TCGGGGAAGC GGTTGCCAAG AGGTTCCATC TGCCAGGTAT
2881 CAGGCAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA
2941 TGATAAACCG GGCGCGGTCG GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT
3001 GGATACCGGG AAAACGCTGG GCGTTAATCA AAGAGGCGAA CTGTGTGTGA GAGGTCCTAT
3061 GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGATGG
3121 ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC GAACACTTCT TCATCGTTGA
3181 CCGCCTGAAG TCTCTGATTA AGTACAAAGG CTATCAGGTG GCTCCCGCTG AATTGGAATC
3241 CATCTTGCTC CAACACCCCA ACATCTTCGA CGCAGGTGTC GCAGGTCTTC CCGACGATGA
3301 CGCCGGTGAA CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA
3361 AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC GCGGAGGAGT
3421 TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA CTCGACGCAA GAAAAATCAG
3481 AGAGATCCTC ATAAAGGCCA AGAAGGGCGG AAAGATCGCC GTGTAATTCT AGAGTCGGGG
3541 CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC
3601 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT
3661 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
3721 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA
3781 AATCGATAAG GATCTGAACG ATGGAGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC
3841 GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC
3901 ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA
3961 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA
4021 CACACATTCC ACAGCGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT
4081 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
4141 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
4201 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
4261 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
4321 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG
4381 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
4441 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
4501 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT
4561 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
4621 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
4681 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
4741 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA
4801 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
4861 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
4921 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
4981 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
5041 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
5101 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
5161 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
5221 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA
5281 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
5341 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
5401 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
5461 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
5521 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
5581 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
5641 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
5701 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
5761 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
5821 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
5881 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
5941 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
6001 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
6061 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC
6121 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
6181 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
6241 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
6301 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
6361 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
6421 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
6481 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
6541 ATATTAACGT TTACAATTTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
6601 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC CATGATAAGT AAGTAATATT
6661 AAGGTACGGG AGGTACTTGG AGCGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT
6721 GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA AACAAAACGA
6781 AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC AGAACATTTC
6841 TCTATCGATA
//