LOCUS _990_1222_CAT_Ires 7276 bp ds-DNA circular 03-OCT-2008
DEFINITION .
ACCESSION
VERSION
SOURCE .
ORGANISM .
COMMENT ID 990-1222/CAT standard; circular DNA; ; 7276 BP.
CC This file is created by Vector NTI
CC http://www.invitrogen.com/
CC VNTDATE|493819540|
CC VNTDBDATE|493819540|
CC LSOWNER|
CC VNTAUTHORNAME|Demo User|
FH Key Location/Qualifiers
FH
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
promoter 291..945
/vntifkey="29"
/note="CMV promotor"
/label=CMV\Promotor
/ApEinfo_fwdcolor=#ff8000
/ApEinfo_revcolor=pink
primer_bind 945..964
/label=T7
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
promoter 946..964
/vntifkey="30"
/note="T7 promotor"
/label=T7(1)
/ApEinfo_label=T7
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
misc_feature 913..913
/label=CMV transcriptional start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
misc_feature 962..962
/label=T7 transcriptional start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
misc_feature 2048..2894
/vntifkey="21"
/note=" SV40 small T antigen "
/label=SV40_SM_T
/ApEinfo_fwdcolor=#00ff80
/ApEinfo_revcolor=pink
polyA_signal 2930..3161
/vntifkey="25"
/note="BGH poly A"
/label=BGH\polyA
/ApEinfo_fwdcolor=#c0c0c0
/ApEinfo_revcolor=pink
misc_feature 6362..7222
/label=beta lactamase
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
rep_origin complement(5585..6267)
/label=ColE1 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
polyA_signal complement(2703..2894)
/label=SV40 late polyA
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
promoter 3702..4027
/vntifkey="29"
/note="SV40 promotor"
/label=SV40
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
misc_feature 4063..4857
/label=Kan/Neo
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
rep_origin 3241..3547
/label=F1 ori
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
primer_bind complement(2911..2928)
/label=SP6
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
primer_bind complement(5197..5217)
/label=M13-rev
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
misc_binding complement(5223..5245)
/label=LacO
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
misc_feature 5032..5162
/vntifkey="21"
/note="SV40 poly A"
/label=SV40\polyA
/ApEinfo_fwdcolor=#00ff80
/ApEinfo_revcolor=pink
misc_feature 1060..1292
/label=Hiap2_990-1222
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
misc_feature 1300..1958
/label=CAT
/ApEinfo_fwdcolor=#0000ff
/ApEinfo_revcolor=green
ORIGIN
1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact
121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc
541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg
1021 aattctgcag atatccatca cactggcggc cgcctcgagg agagatactc atcctacctg
1081 aatataaact gagataaatc cagtaaagaa agtgtagtaa attctacata agagtctatc
1141 attgatttct ttttgtggta aaaatcttag ttcatgtgaa gaaatttcat gtgaatgttt
1201 tagctatcaa acagtactgt cacctactca tgcacaaaac tgcctcccaa agacttttcc
1261 caggtccctc gtatcaaaac attaagagta tactcgagat ggagaaaaaa atcactggat
1321 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
1381 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
1441 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
1501 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
1561 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
1621 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
1681 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
1741 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
1801 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
1861 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
1921 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
1981 gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa
2041 ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
2101 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
2161 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
2221 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
2281 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
2341 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
2401 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
2461 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
2521 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
2581 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
2641 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
2701 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
2761 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
2821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
2881 tcttatcatg tctgcctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
2941 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
3001 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
3061 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
3121 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
3181 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
3241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
3301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
3361 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
3421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
3481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
3541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
3601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
3661 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
3721 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
3781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
3841 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
3901 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
3961 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
4021 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
4081 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
4141 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
4201 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
4261 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
4321 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
4381 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
4441 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
4501 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
4561 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
4621 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
4681 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
4741 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
4801 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
4861 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
4921 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
4981 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
5161 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
5221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
5281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
5341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
5401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
5461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
5521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
5641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
5701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
5761 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
5821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
5881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
5941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
6001 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
6061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
6121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
6181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
6241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
6301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
7261 gttattgtct catgag
//