LOCUS pCITE2a 3800 bp ds-DNA circular 20-DEC-2008
SOURCE
ORGANISM
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
promoter 3784..3800
/vntifkey="30"
/label=T7 promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
enhancer 16..518
/vntifkey="9"
/label=CITE
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
protein_bind 589..606
/vntifkey="31"
/label=His tag
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
promoter complement(664..680)
/vntifkey="30"
/label=T3 promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
rep_origin complement(1104..1104)
/vntifkey="33"
/label=origin
/ApEinfo_fwdcolor=#00c0cb
/ApEinfo_revcolor=#00c0cb
CDS complement(1865..2722)
/vntifkey="4"
/label=bla (amp) sequence
/ApEinfo_fwdcolor=#d3ff00
/ApEinfo_revcolor=#d3ff00
rep_origin complement(3161..3616)
/vntifkey="33"
/label=f1 origin
/ApEinfo_fwdcolor=#00c0cb
/ApEinfo_revcolor=#00c0cb
misc_feature 17..516
/label=EMCV IRES
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1..1
/label=T7 promoter transcription start site (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature complement(663..663)
/label=T3 transcription site (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 609..614
/label=BglII site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 622..627
/label=XbaI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 638..643
/label=PstI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 644..649
/label=SphI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 583..588
/label=XhoI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 684..710
/label=end of SV40 poly(A) signal
/ApEinfo_fwdcolor=#ff7cff
/ApEinfo_revcolor=#ff7cff
misc_feature 519..626
/label=fusion protein
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=green
misc_feature 517..522
/label=NcoI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 543..548
/label=BamHI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 555..560
/label=SacI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 562..567
/label=SalI site
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
ORIGIN
1 gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
61 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
481 ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggccaccacc catatggata
541 tcggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
601 caccactgag atctccgatc ctctagagtc gatcgacctg caggcatgca agcttttgtt
661 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
721 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
781 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
841 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
901 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
1021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
1081 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
1141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
1201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
1261 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
1321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
1381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
1441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
1501 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
1561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
1621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
1681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
1741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
1801 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
1861 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
1921 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
1981 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
2041 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
2101 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
2161 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
2221 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
2281 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
2341 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
2401 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
2461 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
2521 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
2581 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
2641 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
2701 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
2761 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
2821 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
2881 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
2941 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
3001 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
3061 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga
3121 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
3181 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
3241 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
3301 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
3361 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
3421 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
3481 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
3541 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
3601 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
3661 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
3721 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
3781 ttgtaatacg actcactata
//