LOCUS pbetagal_cat 11144 bp ds-DNA circular 28-JUL-2009
SOURCE
ORGANISM
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_feature 5945..6791
/vntifkey="21"
/note=" SV40 small T antigen "
/label=SV40_SM_T
/ApEinfo_fwdcolor=#00ff80
/ApEinfo_revcolor=pink
CDS 5196..5855
/vntifkey="4"
/label=CAT
/ApEinfo_fwdcolor=#0000ff
/ApEinfo_revcolor=#c0c0c0
misc_feature complement(4562..5141)
/label=APAF-1
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
promoter 320..974
/vntifkey="29"
/note="CMV promotor"
/label=CMV\Promotor
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
promoter 975..993
/vntifkey="30"
/note="T7 promotor"
/label=T7
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
polyA_signal 6827..7058
/vntifkey="25"
/note="BGH poly A"
/label=BGH\polyA
/ApEinfo_fwdcolor=#c0c0c0
/ApEinfo_revcolor=pink
promoter 7599..7924
/vntifkey="29"
/note="SV40 promotor"
/label=SV40
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
misc_feature 8929..9059
/vntifkey="21"
/note="SV40 poly A"
/label=SV40\polyA
/ApEinfo_fwdcolor=#00ff80
/ApEinfo_revcolor=pink
misc_feature 1130..4273
/label=LacZ
/ApEinfo_fwdcolor=#ffff00
/ApEinfo_revcolor=green
primer_bind complement(9094..9114)
/label=M13-rev
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
primer_bind 974..993
/label=T7(1)
/ApEinfo_label=T7
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
primer_bind complement(6808..6825)
/label=SP6
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
rep_origin complement(9482..10164)
/label=ColE1 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
rep_origin 7138..7444
/label=F1 ori
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
CDS 1307..1375
/label=LacZ alpha
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
misc_binding complement(9120..9142)
/label=LacO
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
polyA_signal complement(6600..6791)
/label=SV40 late polyA
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
misc_feature 991..991
/label=T7 transcriptional start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
misc_feature 942..942
/label=CMV transcriptional start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
misc_feature 10259..11119
/label=beta lactamase
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
misc_feature 7960..8754
/label=Kan/Neo
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
ORIGIN
1 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
61 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
121 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt
181 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
241 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
301 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
361 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
421 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
481 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
781 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
841 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
901 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
961 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
1021 tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc
1081 gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa gaagtcacca tgtcgtttac
1141 tttgaccaac aagaacgtga ttttcgttgc cggtctggga ggcattggtc tggacaccag
1201 caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
1261 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
1321 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
1381 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
1441 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
1501 caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
1561 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
1621 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
1681 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
1741 agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca
1801 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
1861 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
1921 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
1981 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
2041 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
2101 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
2161 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
2221 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
2281 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
2341 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
2401 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
2461 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
2521 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
2581 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
2641 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
2701 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
2761 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
2821 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
2881 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
2941 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
3001 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
3061 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
3121 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
3181 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
3241 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
3301 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
3361 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
3421 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
3481 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
3541 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
3601 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
3661 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
3721 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
3781 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
3841 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
3901 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
3961 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
4021 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
4081 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
4141 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
4201 cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg
4261 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
4321 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta
4381 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca
4441 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt
4501 ccaaccgctg tttggtctgc tttctgacaa actcggcctc gactctaggc ggccgctcga
4561 gcatcttccc tcagatcttt ctctctctga gctgtcaacc atgagccaag cctttgcgcc
4621 taggtcttag tggggagggc ggcactgccg ggacaagact gggagactcc aggccggtgg
4681 agggaaacgg caacaggagt tttccaccgg gaattagggg tgcagggctc tgtctcgcca
4741 catacccttc gccccggggc aggtccgggc ctcacaggcg cctcccctgg atctctgcag
4801 ccccggggcg cacgaagacc cccagggacc tccgaaggtt ggcgaggtgg gcgtccaccc
4861 aacgcagcct gaccccacag tcccggagcg ccgcggtgag gcccggccca cgggccgccc
4921 tccggtggac cccactactg gacacaaagg gaggaggtct tcccggcctg tggcgccctt
4981 cccccgacgg cggctgcacc ccggagggag cccggcgcta ctccggatgc cgctgcggca
5041 cctcaagtct tcgcgggtcg ccgactgccc agactcccca cctctggttc tatccctttt
5101 gcccgggata gagcagtcac gtccactcgc tacctcttct tctcgagcta gaggatctga
5161 gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
5221 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
5281 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
5341 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
5401 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
5461 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
5521 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
5581 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
5641 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
5701 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
5761 ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
5821 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
5881 cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
5941 gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
6001 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
6061 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
6121 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
6181 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
6241 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
6301 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
6361 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
6421 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
6481 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
6541 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
6601 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
6661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
6721 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
6781 tatcatgtct gcctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
6841 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
6901 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
6961 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
7021 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
7081 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
7141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
7201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
7261 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
7321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
7381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
7441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
7501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
7561 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa
7621 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
7681 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
7741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
7801 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
7861 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
7921 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
7981 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
8041 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
8101 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
8161 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
8221 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
8281 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
8341 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
8401 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
8461 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
8521 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
8581 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
8641 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
8701 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
8761 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
8821 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
8881 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
8941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
9001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
9061 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
9121 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
9181 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
9241 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
9301 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
9361 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
9421 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
9481 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
9541 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
9601 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
9661 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
9721 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
9781 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
9841 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
9901 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
9961 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
10021 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
10081 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
10141 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
10201 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
10261 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
10321 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
10381 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
10441 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
10501 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
10561 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
10621 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
10681 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
10741 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
10801 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
10861 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
10921 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
10981 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
11041 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
11101 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttga
//