LOCUS _320__229 6571 bp ds-DNA linear 01-JUL-2008
DEFINITION
ACCESSION -320/-229_
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown Unclassified.
REFERENCE 1 (bases 1 to 6571)
AUTHORS Self
JOURNAL Unpublished.
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
CDS 1590..2525
/gene="Rluc"
/label=Rluc
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
CDS 2578..2664
/gene="n-myc"
/label=n-myc
/ApEinfo_fwdcolor=#ff8080
/ApEinfo_revcolor=#00ff00
CDS 2666..4318
/gene="FFluc"
/label=FFluc
/ApEinfo_fwdcolor=#ff80ff
/ApEinfo_revcolor=#ff8000
misc_feature 4350..4571
/label=SV40 Late poly(A) signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 1119..1320
/label=SV40 Promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 1217..1266
/label=SV40 transcription start sites region
/ApEinfo_fwdcolor=#ffb1be
/ApEinfo_revcolor=#ffb1be
misc_feature 4591..4827
/label=Enhancer
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 1524..1542
/label=T7 promoter
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1072..1112
/label=Multiple cloning region
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1347..1479
/label=chimeric intron
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1541..1541
/label=T7 transcription start (+1)
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 1217..1217
/label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1255..1255
/label=SV40 transcription start (+1)(1)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#fe00ff
/ApEinfo_revcolor=#fe00ff
misc_feature 1261..1261
/label=SV40 transcription start (+1)(2)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1266..1266
/label=SV40 transcription start (+1)(3)
/ApEinfo_label=SV40 transcription start (+1)
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
rep_origin complement(5127..5809)
/label=ColE1 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
rep_origin 342..648
/label=F1 ori
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
CDS complement(782..850)
/label=LacZ alpha
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
CDS complement(5907..6566)
/label=AmpR
/ApEinfo_fwdcolor=yellow
/ApEinfo_revcolor=yellow
polyA_signal 4350..4541
/label=SV40 late polyA
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
ORIGIN
1 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
61 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
121 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
181 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC
241 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA
301 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
361 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
421 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG
481 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
541 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT
601 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA
661 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
721 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT
781 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
841 TATTACGCCA GCCCAAGCTA CCATGATAAG TAAGTAATAT TAAGGTACGG GAGGTACTTG
901 GAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA
961 ATCGATAGTA CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA
1021 ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT CTCTATCGAT AGGTACCGAG
1081 CTCTTACGCG TGCTAGCCCG GGCTCGAGAT CTGCGATCTG CATCTCAATT AGTCAGCAAC
1141 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
1201 TCCGCCCCAT CGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC
1261 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
1321 GAAGTTGGTC GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG
1381 ACCAATAGAA ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA
1441 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT
1501 ACAGCTCTTA AGGCTAGAGT ACTTAATACG ACTCACTATA GGCTAGGCTT GGCATTCCGG
1561 TACTGAATTG ATATCAAGCT CTAGCCACCA TGACTTCGAA AGTTTATGAT CCAGAACAAA
1621 GGAAACGGAT GATAACTGGT CCGCAGTGGT GGGCCAGATG TAAACAAATG AATGTTCTTG
1681 ATTCATTTAT TAATTATTAT GATTCAGAAA AACATGCAGA AAATGCTGTT ATTTTTTTAC
1741 ATGGTAACGC GGCCTCTTCT TATTTATGGC GACATGTTGT GCCACATATT GAGCCAGTAG
1801 CGCGGTGTAT TATACCAGAC CTTATTGGTA TGGGCAAATC AGGCAAATCT GGTAATGGTT
1861 CTTATAGGTT ACTTGATCAT TACAAATATC TTACTGCATG GTTTGAACTT CTTAATTTAC
1921 CAAAGAAGAT CATTTTTGTC GGCCATGATT GGGGTGCTTG TTTGGCATTT CATTATAGCT
1981 ATGAGCATCA AGATAAGATC AAAGCAATAG TTCACGCTGA AAGTGTAGTA GATGTGATTG
2041 AATCATGGGA TGAATGGCCT GATATTGAAG AAGATATTGC GTTGATCAAA TCTGAAGAAG
2101 GAGAAAAAAT GGTTTTGGAG AATAACTTCT TCGTGGAAAC CATGTTGCCA TCAAAAATCA
2161 TGAGAAAGTT AGAACCAGAA GAATTTGCAG CATATCTTGA ACCATTCAAA GAGAAAGGTG
2221 AAGTTCGTCG TCCAACATTA TCATGGCCTC GTGAAATCCC GTTAGTAAAA GGTGGTAAAC
2281 CTGACGTTGT ACAAATTGTT AGGAATTATA ATGCTTATCT ACGTGCAAGT GATGATTTAC
2341 CAAAAATGTT TATTGAATCG GACCCAGGAT TCTTTTCCAA TGCTATTGTT GAAGGTGCCA
2401 AGAAGTTTCC TAATACTGAA TTTGTCAAAG TAAAAGGTCT TCATTTTTCG CAAGAAGATG
2461 CACCTGATGA AATGGGAAAA TATATCAAAT CGTTCGTTGA GCGAGTTCTC AAAAATGAAC
2521 AATAATTCTA GAGCTTATCG ATACCGTCGA CCTCGAATCA CTAGTCAGCT GGAATTCGTC
2581 TGGACGCGCT GGGTGGATGC GGGGGGCTCC TGGGAACTGT GTTGGAGCCG AGCAAGCGCT
2641 AGCCAGGCGC AAGCGCGCAC AGACCATGGA AGACGCCAAA AACATAAAGA AAGGCCCGGC
2701 GCCATTCTAT CCGCTGGAAG ATGGAACCGC TGGAGAGCAA CTGCATAAGG CTATGAAGAG
2761 ATACGCCCTG GTTCCTGGAA CAATTGCTTT TACAGATGCA CATATCGAGG TGGACATCAC
2821 TTACGCTGAG TACTTCGAAA TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT
2881 GAATACAAAT CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
2941 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT ATAATGAACG
3001 TGAATTGCTC AACAGTATGG GCATTTCGCA GCCTACCGTG GTGTTCGTTT CCAAAAAGGG
3061 GTTGCAAAAA ATTTTGAACG TGCAAAAAAA GCTCCCAATC ATCCAAAAAA TTATTATCAT
3121 GGATTCTAAA ACGGATTACC AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT
3181 ACCTCCCGGT TTTAATGAAT ACGATTTTGT GCCAGAGTCC TTCGATAGGG ACAAGACAAT
3241 TGCACTGATC ATGAACTCCT CTGGATCTAC TGGTCTGCCT AAAGGTGTCG CTCTGCCTCA
3301 TAGAACTGCC TGCGTGAGAT TCTCGCATGC CAGAGATCCT ATTTTTGGCA ATCAAATCAT
3361 TCCGGATACT GCGATTTTAA GTGTTGTTCC ATTCCATCAC GGTTTTGGAA TGTTTACTAC
3421 ACTCGGATAT TTGATATGTG GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT
3481 GTTTCTGAGG AGCCTTCAGG ATTACAAGAT TCAAAGTGCG CTGCTGGTGC CAACCCTATT
3541 CTCCTTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT TACACGAAAT
3601 TGCTTCTGGT GGCGCTCCCC TCTCTAAGGA AGTCGGGGAA GCGGTTGCCA AGAGGTTCCA
3661 TCTGCCAGGT ATCAGGCAAG GATATGGGCT CACTGAGACT ACATCAGCTA TTCTGATTAC
3721 ACCCGAGGGG GATGATAAAC CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA
3781 GGTTGTGGAT CTGGATACCG GGAAAACGCT GGGCGTTAAT CAAAGAGGCG AACTGTGTGT
3841 GAGAGGTCCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA ACGCCTTGAT
3901 TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC TGGGACGAAG ACGAACACTT
3961 CTTCATCGTT GACCGCCTGA AGTCTCTGAT TAAGTACAAA GGCTATCAGG TGGCTCCCGC
4021 TGAATTGGAA TCCATCTTGC TCCAACACCC CAACATCTTC GACGCAGGTG TCGCAGGTCT
4081 TCCCGACGAT GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
4141 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG CGAAAAAGTT
4201 GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC
4261 AAGAAAAATC AGAGAGATCC TCATAAAGGC CAAGAAGGGC GGAAAGATCG CCGTGTAATT
4321 CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG
4381 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
4441 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
4501 TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA
4561 CAAATGTGGT AAAATCGATA AGGATCTGAA CGATGGAGCG GAGAATGGGC GGAACTGGGC
4621 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA
4681 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT
4741 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA
4801 CACCCTAACT GACACACATT CCACAGCGGA TCCGTCGACC GATGCCCTTG AGAGCCTTCA
4861 ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG
4921 TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC
4981 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
5041 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
5101 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
5161 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
5221 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
5281 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
5341 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
5401 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
5461 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
5521 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
5581 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
5641 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
5701 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
5761 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
5821 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
5881 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
5941 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
6001 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
6061 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
6121 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
6181 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
6241 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
6301 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
6361 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
6421 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
6481 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
6541 CATAGCAGAA CTTTAAAAGT GCTCATCATT G
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