LOCUS            deletion_1_31        6581 bp ds-DNA   linear       30-JUN-2008
DEFINITION  
ACCESSION   
KEYWORDS    
SOURCE      Unknown.
  ORGANISM  Unknown Unclassified.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             3178..3837
                     /gene="CAT"
                     /label=CAT
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#d0abd6
     CDS             4828..6480
                     /gene="FFLuc"
                     /label=FFLuc
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#d0abd6
     primer_bind     complement(24..41)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00ffff
     primer_bind     3078..3098
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     3104..3129
                     /label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      2028..2710
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             112..180
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     misc_binding    3050..3072
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     CDS             1271..1930
                     /label=beta lactamase
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             3178..3834
                     /label=CmR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    complement(183..636)
                     /label=f1 ori
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#ff0080
     CDS             3952..4820
                     /gene="BVDV"
                     /label=BVDV
                     /ApEinfo_fwdcolor=#0000a0
                     /ApEinfo_revcolor=#d0abd6
     misc_feature    3128..3128
                     /label=SP6 transcription start
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
ORIGIN
        1 TATAGTGAGT CGTATTACAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
       61 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT
      121 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
      181 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT
      241 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA
      301 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA
      361 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT
      421 AATCAAGTTT TTTGCGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC
      481 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG
      541 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA
      601 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCTG ATGCGGTATT TTCTCCTTAC
      661 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC
      721 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
      781 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
      841 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT
      901 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
      961 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     1021 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     1081 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     1141 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     1201 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     1261 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     1321 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     1381 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     1441 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     1501 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     1561 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     1621 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     1681 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     1741 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     1801 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     1861 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     1921 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     1981 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     2041 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     2101 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     2161 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     2221 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     2281 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     2341 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     2401 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     2461 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     2521 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     2581 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     2641 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     2701 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
     2761 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
     2821 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
     2881 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
     2941 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
     3001 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
     3061 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TATTTAGGTG
     3121 ACACTATAGA ATACTCAAGC TTATGCATGC GGCCGCTCAG GAGCTAAGGA AGCTAAAATG
     3181 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT
     3241 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT
     3301 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC
     3361 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG
     3421 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG
     3481 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG
     3541 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT
     3601 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC
     3661 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC GCAAGGCGAC
     3721 AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TCTGTGATGG CTTCCATGTC
     3781 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT
     3841 TTTTAAGGCA GTTATTGGTG CCCTTAAACG CCTGGTTCGT ACGCCTGAAT AAGTGATAAT
     3901 AAGCGGATGA ATGGCAGAAA TTCGAGCGGC CGCATCTAGA GGGCCCGGAT CCGTATTGGG
     3961 CAATTAAAAA TAATAATTAG GCCTAGGGAA CAAATCCCTC TCAGCGAAGG CCGAAAAGAG
     4021 GCTAGCCATG CCCTTAGTAG GACTAGCATA ATGAGGGGGG TAGCAACAGT GGTGAGTTCG
     4081 TTGGATGGCT TAAGCCCTGA GTACAGGGTA GTCGTCAGTG GTTCGACGCC TTGGAATAAA
     4141 GGTCTCGAGA TGCCACGTGG ACGAGGGCAT GCCCAAAGCA CATCTTAACC TGAGCGGGGG
     4201 TCGCCCAGGT AAAAGCAGTT TTAACCGACT GTTACGAATA CAGCCTGATA GGGTGCTGCA
     4261 GAGGCCCACT GTATTGCTAC TAAAAATCTC TGCTGTACAT GGCACATGGA GTTGATCACA
     4321 AATGAACTTT TATACAAAAC ATACAAACAA AAACCCGTCG GGGTGGAGGA ACCTGTTTAT
     4381 GATCAGGCAG GTGATCCCTT ATTTGGTGAA AGGGGAGCAG TCCACCCTCA ATCGACGCTA
     4441 AAGCTCCCAC ACAAGAGAGG GGAACGCGAT GTTCCAACCA ACTTGGCATC CTTACCAAAA
     4501 AGAGGTGACT GCAGGTCGGG TAATAGCAGA GGACCTGTGA GCGGGATCTA CCTGAAGCCA
     4561 GGGCCACTAT TTTACCAGGA CTATAAAGGT CCCGTCTATC ACAGGGCCCC GCTGGAGCTC
     4621 TTTGAGGAGG GATCCATGTG TGAAACGACT AAACGGATAG GGAGAGTAAC TGGAAGTGAC
     4681 GGAAAGCTGT ACCACATTTA TGTGTGTATA GATGGATGTA TAATAATAAA AAGTGCCACG
     4741 AGAAGTTACC AAAGGGTGTT CAGGTGGGTC CATAATAGGC TTGACTGCCC TCTATGGGTC
     4801 ACAACTTGCT CAGACACGAA GATCCAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
     4861 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GGCTATGAAG
     4921 AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGTGAACATC
     4981 ACGTACGCGG AATACTTCGA AATGTCCGTT CGGTTGGCAG AAGCTATGAA ACGATATGGG
     5041 CTGAATACAA ATCACAGAAT CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG
     5101 GTGTTGGGCG CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
     5161 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT TTCCAAAAAG
     5221 GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA TAATCCAGAA AATTATTATC
     5281 ATGGATTCTA AAACGGATTA CCAGGGATTT CAGTCGATGT ACACGTTCGT CACATCTCAT
     5341 CTACCTCCCG GTTTTAATGA ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA
     5401 ATTGCACTGA TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
     5461 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG CAATCAAATC
     5521 ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC ACGGTTTTGG AATGTTTACT
     5581 ACACTCGGAT ATTTGATATG TGGATTTCGA GTCGTCTTAA TGTATAGATT TGAAGAAGAG
     5641 CTGTTTTTAC GATCCCTTCA GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA
     5701 TTTTCATTCT TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
     5761 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC AAAACGCTTC
     5821 CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA CTACATCAGC TATTCTGATT
     5881 ACACCCGAGG GGGATGATAA ACCGGGCGCG GTCGGTAAAG TTGTTCCATT TTTTGAAGCG
     5941 AAGGTTGTGG ATCTGGATAC CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT
     6001 GTCAGAGGAC CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
     6061 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA AGACGAACAC
     6121 TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA AAGGATATCA GGTGGCCCCC
     6181 GCTGAATTGG AATCGATATT GTTACAACAC CCCAACATCT TCGACGCGGG CGTGGCAGGT
     6241 CTTCCCGACG ATGACGCCGG TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG
     6301 ACGATGACGG AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
     6361 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG AAAACTCGAC
     6421 GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG GCGGAAAGTC CAAATTGTAA
     6481 AATGTAACTG TATTCAGCGA TGACGAAATT CTTAGCTATT GTAATCCTCC GAGGCCTCGA
     6541 GGTCGACGAA TTCGAGCTCG GCCGACTTGG CCAATTCGCC C 
//