LOCUS pBi456F 6168 bp ds-DNA linear 30-JUN-2008
DEFINITION
ACCESSION pBi456F
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown Unclassified.
REFERENCE 1 (bases 1 to 6612)
AUTHORS Self
JOURNAL Unpublished.
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
CDS 3178..3837
/gene="CAT"
/label=CAT
/ApEinfo_fwdcolor=#ffff00
/ApEinfo_revcolor=#d0abd6
CDS 4415..6067
/gene="FFLuc"
/label=FFLuc
/ApEinfo_fwdcolor=#ff8000
/ApEinfo_revcolor=#d0abd6
primer_bind complement(24..41)
/label=M13-fwd
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=#00ffff
primer_bind 3078..3098
/label=M13-rev
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
primer_bind 3104..3120
/label=SP6
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
primer_bind 3121..3129
/label=SP6(1)
/ApEinfo_label=SP6
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
rep_origin 2028..2710
/label=ColE1 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
CDS 112..180
/label=LacZ alpha
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
misc_binding 3050..3072
/label=LacO
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
CDS 1271..1930
/label=beta lactamase
/ApEinfo_fwdcolor=yellow
/ApEinfo_revcolor=yellow
CDS 3178..3834
/label=CmR
/ApEinfo_fwdcolor=yellow
/ApEinfo_revcolor=yellow
misc_feature complement(183..636)
/label=f1 ori
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=#ff0080
misc_feature 3128..3128
/label=SP6 transcription start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
CDS 3954..4407
/gene="BVDV genome segment"
/product="bovine viral diarrhea virus 1 polyprotein; The"
/label=bovine viral diarrhea virus 1 polyprotein
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#00ff00
ORIGIN
1 TATAGTGAGT CGTATTACAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
61 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT
121 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
181 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT
241 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA
301 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA
361 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT
421 AATCAAGTTT TTTGCGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC
481 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG
541 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA
601 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCTG ATGCGGTATT TTCTCCTTAC
661 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC
721 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
781 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
841 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT
901 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
961 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
1021 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
1081 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
1141 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
1201 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
1261 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
1321 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
1381 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
1441 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
1501 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
1561 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
1621 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
1681 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
1741 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
1801 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
1861 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
1921 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
1981 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
2041 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
2101 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
2161 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
2221 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
2281 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
2341 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
2401 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
2461 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
2521 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
2581 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
2641 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
2701 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
2761 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
2821 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
2881 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
2941 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
3001 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
3061 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TATTTAGGTG
3121 ACACTATAGA ATACTCAAGC TTATGCATGC GGCCGCTCAG GAGCTAAGGA AGCTAAAATG
3181 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT
3241 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT
3301 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC
3361 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG
3421 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG
3481 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG
3541 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT
3601 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC
3661 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC GCAAGGCGAC
3721 AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TCTGTGATGG CTTCCATGTC
3781 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT
3841 TTTTAAGGCA GTTATTGGTG CCCTTAAACG CCTGGTTCGT ACGCCTGAAT AAGTGATAAT
3901 AAGCGGATGA ATGGCAGAAA TTCGAGCGGC CGCATCTAGA GGGCCCGGAT CCTGAAGTAA
3961 TACCAGGGTC GGTCTGGAAT CTAGGCAAAT ATGTATGTAT AAGACCAAAT TGGTGGCCTT
4021 ATGAGACAAC TGTAGTGTTG GCATTTGAAG AGGTGAGCCA GGTGGTGAAG TTAGTGTTGA
4081 GGGCACTCAG AGATTTAACA CGCATTTGGA ACGCTGCAAC AACTACTGCT TTTTTAGTAT
4141 GCCTTGTTAA GATAGTCAGG GGCCAGATGG TACAGGGCAT TCTGTGGCTA CTATTGATAA
4201 CAGGGGTACA AGGGCACTTG GATTGCAAAC CTGAATTCTC GTATGCCATA GCAAAGGACG
4261 AAAGAATTGG TCAACTGGGG GCTGAAGGCC TTACCACCAC TTGGAAGGAA TACTCACCTG
4321 GAATGAAGCT GGAAGACACA ATGGTCATTG CTTGGTGCGA AGATGGGAAG TTAATGTACC
4381 TCCAAAGATG CACGAGAGAA ACCAGATGAT CCAAATGGAA GACGCCAAAA ACATAAAGAA
4441 AGGCCCGGCG CCATTCTATC CTCTAGAGGA TGGAACCGCT GGAGAGCAAC TGCATAAGGC
4501 TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT ACAGATGCAC ATATCGAGGT
4561 GAACATCACG TACGCGGAAT ACTTCGAAAT GTCCGTTCGG TTGGCAGAAG CTATGAAACG
4621 ATATGGGCTG AATACAAATC ACAGAATCGT CGTATGCAGT GAAAACTCTC TTCAATTCTT
4681 TATGCCGGTG TTGGGCGCGT TATTTATCGG AGTTGCAGTT GCGCCCGCGA ACGACATTTA
4741 TAATGAACGT GAATTGCTCA ACAGTATGAA CATTTCGCAG CCTACCGTAG TGTTTGTTTC
4801 CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAA TTACCAATAA TCCAGAAAAT
4861 TATTATCATG GATTCTAAAA CGGATTACCA GGGATTTCAG TCGATGTACA CGTTCGTCAC
4921 ATCTCATCTA CCTCCCGGTT TTAATGAATA CGATTTTGTA CCAGAGTCCT TTGATCGTGA
4981 CAAAACAATT GCACTGATAA TGAATTCCTC TGGATCTACT GGGTTACCTA AGGGTGTGGC
5041 CCTTCCGCAT AGAACTGCCT GCGTCAGATT CTCGCATGCC AGAGATCCTA TTTTTGGCAA
5101 TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA TTCCATCACG GTTTTGGAAT
5161 GTTTACTACA CTCGGATATT TGATATGTGG ATTTCGAGTC GTCTTAATGT ATAGATTTGA
5221 AGAAGAGCTG TTTTTACGAT CCCTTCAGGA TTACAAAATT CAAAGTGCGT TGCTAGTACC
5281 AACCCTATTT TCATTCTTCG CCAAAAGCAC TCTGATTGAC AAATACGATT TATCTAATTT
5341 ACACGAAATT GCTTCTGGGG GCGCACCTCT TTCGAAAGAA GTCGGGGAAG CGGTTGCAAA
5401 ACGCTTCCAT CTTCCAGGGA TACGACAAGG ATATGGGCTC ACTGAGACTA CATCAGCTAT
5461 TCTGATTACA CCCGAGGGGG ATGATAAACC GGGCGCGGTC GGTAAAGTTG TTCCATTTTT
5521 TGAAGCGAAG GTTGTGGATC TGGATACCGG GAAAACGCTG GGCGTTAATC AGAGAGGCGA
5581 ATTATGTGTC AGAGGACCTA TGATTATGTC CGGTTATGTA AACAATCCGG AAGCGACCAA
5641 CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC ATAGCTTACT GGGACGAAGA
5701 CGAACACTTC TTCATAGTTG ACCGCTTGAA GTCTTTAATT AAATACAAAG GATATCAGGT
5761 GGCCCCCGCT GAATTGGAAT CGATATTGTT ACAACACCCC AACATCTTCG ACGCGGGCGT
5821 GGCAGGTCTT CCCGACGATG ACGCCGGTGA ACTTCCCGCC GCCGTTGTTG TTTTGGAGCA
5881 CGGAAAGACG ATGACGGAAA AAGAGATCGT GGATTACGTC GCCAGTCAAG TAACAACCGC
5941 GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA CCGAAAGGTC TTACCGGAAA
6001 ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC AAGAAGGGCG GAAAGTCCAA
6061 ATTGTAAAAT GTAACTGTAT TCAGCGATGA CGAAATTCTT AGCTATTGTA ATCCTCCGAG
6121 GCCTCGAGGT CGACGAATTC GAGCTCGGCC GACTTGGCCA ATTCGCCC
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