LOCUS pRst-LEF1_12-F 6700 bp ds-DNA circular SYN 14-JUN-2007
DEFINITION .
ACCESSION .
VERSION .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_feature 698..1654
/label=RLuc
/ApEinfo_fwdcolor=#00ff06
/ApEinfo_revcolor=#00ff06
misc_feature 337..409
/label=SV40_promoter_and_enhancer
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 11..253
/label=SV40_promoter
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 669..688
/label=T7_promoter
/ApEinfo_fwdcolor=#0000ff
/ApEinfo_revcolor=#0000ff
misc_feature 687..687
/label=T7_transcription_start
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 493..625
/label=chimeric_intron
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 254..336
/label=SV40_promoter(1)
/ApEinfo_label=SV40_promoter
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 4638..4859
/label=SV40_late_polyA
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 325..374
/label=Putative_major_transcription_start_sites
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 1660..1696
/label=Multiple_cloning_site
/ApEinfo_fwdcolor=#ff5b00
/ApEinfo_revcolor=#ff5b00
misc_feature 1762..1767
/label=HindIII
/ApEinfo_fwdcolor=#ff9b86
/ApEinfo_revcolor=#ff9b86
misc_feature 410..421
/label=SV40_promoter_enhancer
/ApEinfo_fwdcolor=#ff00ff
/ApEinfo_revcolor=#ff00ff
misc_feature 2955..2955
/label=NcoI
/ApEinfo_fwdcolor=#e6b83e
/ApEinfo_revcolor=#e6b83e
misc_feature 1662..1767
/label=hairpin
/ApEinfo_fwdcolor=#ffff00
/ApEinfo_revcolor=#ffff00
misc_feature 1769..1773
/label=BglII_cleaved
/ApEinfo_fwdcolor=#ee9e66
/ApEinfo_revcolor=#ee9e66
misc_feature 1656..1656
/label=BglII_cleaved(1)
/ApEinfo_label=BglII_cleaved
/ApEinfo_fwdcolor=#ec9c4b
/ApEinfo_revcolor=#ec9c4b
misc_feature 2955..2957
/label=AfeI-cleaved-right_part
/ApEinfo_fwdcolor=#ecc376
/ApEinfo_revcolor=#ecc376
misc_feature 1657..1661
/label=BamHI-cleaved_right
/ApEinfo_fwdcolor=#dba96b
/ApEinfo_revcolor=#dba96b
misc_feature 4618..4623
/label=XbaI
/ApEinfo_fwdcolor=#e1d8db
/ApEinfo_revcolor=#e1d8db
misc_feature 1768..1768
/label=BamHI-cleaved_left
/ApEinfo_fwdcolor=#dba370
/ApEinfo_revcolor=#dba370
misc_feature 2964..4616
/label=fluc
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=green
misc_feature 2958..2963
/label=BamHI
/ApEinfo_fwdcolor=#d9c3be
/ApEinfo_revcolor=#d9c3be
misc_feature 2351..2738
/label=LEF1
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1777..2182
/label=LEF1(1)
/ApEinfo_label=LEF1
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 2829..2954
/label=LEF1(2)
/ApEinfo_label=LEF1
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 2240..2331
/label=LEF1(3)
/ApEinfo_label=LEF1
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 1777..1800
/label=FWD-primer_5b_685
/ApEinfo_fwdcolor=#ceffff
/ApEinfo_revcolor=#ceffff
misc_feature 2931..2954
/label=REV-primer_5b_1863
/ApEinfo_fwdcolor=#d6e4ff
/ApEinfo_revcolor=#d6e4ff
ORIGIN
1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
661 tagagtacTT AATACGACTC ACTATAGGct agccaccatG acttcgaaag tttatgatcc
721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
1621 aaatgaacaa atGtcgaTCG ACgggggccc ctaggAgatc cAAGCTTATC GATTTCGAAC
1681 CCGGGGTACC GAATTCCTCG AGTCTAGAGG AgcatgcTCC TCTAGACTCG AGGAATTCGG
1741 TACCCCGGGT TCGAAATCGA TAAGCTTgGA TCTAGCGCGC GGGAGGAGGA GAAGCAGTGG
1801 GGAGGCGCAG CCGCTCAcCT GCGGGGCAGG GCGCGGAGGA GGGACCCGGN TGcGcGCTCT
1861 CGGGCCGAGG AACCAGGACG cGCCCGGAGC CTCGCACGCG GCCAAGCTCG GGCGTCCCCT
1921 CCCCTCGGCC GGGCGAACTC AAGGGGCGCA GCTCTTTGCT TTGACAGAGC TGGCCGGCGG
1981 AGGCGTGCAG AGCGGCGAGC CGGCGAGCCA GGCTGAGAAA CTCGACCCGG GAACAAAGAG
2041 GGGTCGGACT GAGTGTGTGT GTCGGCTCGA GCTCCGGGNA GAGGCATTTG CCCGAGGNCC
2101 CCGCTGTGAC TCCCCGAGAC TCCGCAGTGC CCTCCACTGN GGAGTCCCCG CGCTTGCCGG
2161 NAAAAACTTT ATTCTTGGCA AACTTCTCTT TCTCTTCCCC TCCTCCTCGG CCCCCATCTT
2221 CTGCTCCTCC TCCTTCTCTA GCAGATTAAA TGAGCCTCGA GAAGAAAAAC CGAAGCGAAA
2281 GGGAAGAAAA TAAGAAGATC TAAAACGGAC ATCTCCAGCG TGGGTGGCTC CTTTTTCTTT
2341 TTCTTTTTTT CCCACCCTTC AGGAAGTGGA CGTTTCGTTA TCTTCTGATC CTTGCACCTT
2401 CTTTTGGGGC AAACGGGGCC CTTCTGCCCA GATCCCCTCT CTTTTCTCGG AAAACAAACT
2461 ACTAAGTCGG CATCCGGGGT AACTACAGTG GAGAGGGTTT CCGCGGAGAC GCGCCGCCGG
2521 ACCCTCCTCT GCACTTTGGG GAGGCGTGCT CCCTCCAGAA CCGGCGTTCT CCGCGCGCAA
2581 ATCCCGGCGA CGCGGGGTCG CGGGGTGGCC GCCGGGGCAG CCTCGTCTAG CGCGCGCCGC
2641 GCAGACGCCC CCGGAGTCGC CAGCTACCGC AGCCCTCGCC GCCCAGTGCC CTTCGGCCTC
2701 GGGGCGGGCG CCTGCGTCGG TCTCCGCGAA GCGGGAAAGC GCGGCGGCCG CCGGGATTCG
2761 GGCGCCGCGG CAGCTGCTCC GGCTGCCGGC CGGCGGCCCC GCGCTCGCCC GCCCCGCTTC
2821 CGCCCGCTGT CCTGCTGCAC GAACCCTTCC AACTCTCCTT TCCTCCCCCA CCCTTGAGTT
2881 ACCCCTCTGT CTTTCCTGCT GTTGCGCGGG TGCTCCCACA GCGGAGCGGA GATTACAGAG
2941 CCGCCGGGAT GCCCGCTGGA TCCATGGaag acgccaaaaa cataaagaaa ggcccggcgc
3001 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat
3061 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt
3121 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
3181 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
3241 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
3301 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt
3361 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg
3421 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
3481 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg
3541 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata
3601 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
3661 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
3721 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
3781 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct
3841 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
3901 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc
3961 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac
4021 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
4081 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga
4141 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
4201 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
4261 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg
4321 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc
4381 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
4441 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
4501 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
4561 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaatTCT
4621 AGAgcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
4681 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
4741 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
4801 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
4861 aatcgataag tttaaacaga tccaggtggc acttttcggg gaaatgtgcg cggaacccct
4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6661 ttcctggcct tttgctggcc ttttgctcac atggctcgac
//