LOCUS pEGFP-CAT-EMCV_137 6079 bp ds-DNA circular SYN 14-JUN-2007
DEFINITION .
ACCESSION .
VERSION .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_feature 8..582
/label=immediate early CMV promoter
/ApEinfo_fwdcolor=#ff1928
/ApEinfo_revcolor=#ff1928
misc_feature 583..589
/label=immediate early CMV promoter(1)
/ApEinfo_label=immediate early CMV promoter
/ApEinfo_fwdcolor=#ff1928
/ApEinfo_revcolor=#ff1928
misc_feature 3791..3926
/label=SV40 origin of replication
/ApEinfo_fwdcolor=#eb3c34
/ApEinfo_revcolor=#eb3c34
misc_feature 2898..2903
/label=SV40 early polyA signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 5018..5023
/label=HSV polyA signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 1370..2006
/label=PCR-product-cleaved-HindIII-BamHI
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 3814..3834
/label=21-bp repeat
/ApEinfo_fwdcolor=#ffab80
/ApEinfo_revcolor=#ffab80
misc_feature 2766..2792
/label=rev-primer
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 2936..2948
/label=mRNA ends somewhere here
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3843..3843
/label=major transcription start
/ApEinfo_fwdcolor=#ff21ff
/ApEinfo_revcolor=#ff21ff
misc_feature 1396..1982
/label=EMCV_IRES
/ApEinfo_fwdcolor=#0080c0
/ApEinfo_revcolor=#0080c0
misc_feature 5354..5997
/label=pUC plasmid replication origin
/ApEinfo_fwdcolor=#00ffff
/ApEinfo_revcolor=#00ffff
misc_feature 59..465
/label=enhancer
/ApEinfo_fwdcolor=#ff3b69
/ApEinfo_revcolor=#ff3b69
misc_feature 2995..3450
/label=f1 single strand DNA origin
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 3881..3881
/label=major transcription start(1)
/ApEinfo_label=major transcription start
/ApEinfo_fwdcolor=#ff36ff
/ApEinfo_revcolor=#ff36ff
misc_feature 3624..3695
/label=enhancer (72-bp tandem repeat)
/ApEinfo_fwdcolor=#ff2649
/ApEinfo_revcolor=#ff2649
misc_feature 554..560
/label=TATA box
/ApEinfo_fwdcolor=#ff4899
/ApEinfo_revcolor=#ff4899
misc_feature 3512..3517
/label=-35 region
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3887..3887
/label=major transcription start(2)
/ApEinfo_label=major transcription start
/ApEinfo_fwdcolor=#ff33ff
/ApEinfo_revcolor=#ff33ff
misc_feature 3696..3767
/label=enhancer (72-bp tandem repeat)(1)
/ApEinfo_label=enhancer (72-bp tandem repeat)
/ApEinfo_fwdcolor=#ff5059
/ApEinfo_revcolor=#ff5059
CDS 3975..4769
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="AAB02575.1"
/db_xref="GI:1377913"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
/label=neomycin phosphotransferase
/ApEinfo_fwdcolor=DarkOrange1
/ApEinfo_revcolor=DarkOrange1
misc_feature 3535..3540
/label=-10 region
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3892..3892
/label=major transcription start(3)
/ApEinfo_label=major transcription start
/ApEinfo_fwdcolor=#ff41ff
/ApEinfo_revcolor=#ff41ff
misc_feature 3771..3791
/label=21-bp repeat(1)
/ApEinfo_label=21-bp repeat
/ApEinfo_fwdcolor=#ff7890
/ApEinfo_revcolor=#ff7890
misc_feature 3547..3547
/label=transcription start (+1) of Kan/Neo resistance
/ApEinfo_fwdcolor=#ff4e54
/ApEinfo_revcolor=#ff4e54
misc_feature 5005..5010
/label=HSV polyA signal(1)
/ApEinfo_label=HSV polyA signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3792..3812
/label=21-bp repeat(2)
/ApEinfo_label=21-bp repeat
/ApEinfo_fwdcolor=#ff96a6
/ApEinfo_revcolor=#ff96a6
misc_feature 2025..2744
/label=EGFP
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_feature 2927..2932
/label=SV40 early polyA signal(1)
/ApEinfo_label=SV40 early polyA signal
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 583..583
/label=CMV transcription start (+1)
/ApEinfo_fwdcolor=#ff19ff
/ApEinfo_revcolor=#ff19ff
misc_feature 652..1311
/label=CAT
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
ORIGIN
1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA
601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA
661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG
721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC
781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT
841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG
901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA
961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT
1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT
1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG
1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG
1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA
1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA
1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTATCTAG
1381 GGCGGCCAAT TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG
1441 GAATAAGGCC GGTGTGCGTT TGTCTATATG TGATTTTCCA CCATATTGCC GTCTTTTGGC
1501 AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC
1561 CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA
1621 GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT
1681 GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA
1741 CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA
1801 AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT
1861 CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC
1921 CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAAGC TTGCCACAAC
1981 CCGGGATAAT TCCTGCAGCC AATATGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG
2041 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
2101 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
2161 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA
2221 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
2281 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
2341 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
2401 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
2461 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT
2521 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA
2581 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT
2641 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
2701 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC CGCGACTCTA
2761 GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA
2821 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC
2881 AGCTTATAAT GGTTACAaat aaagcaatag catcacaaat ttcacaaata aagcattttt
2941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
3001 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
3061 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
3121 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
3181 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
3241 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
3301 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
3361 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
3421 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
3481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
3541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
3601 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
3661 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
3721 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
3781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
3841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
3901 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
3961 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
4021 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
4081 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
4141 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
4201 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
4261 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
4321 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
4381 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
4441 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
4501 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
4561 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
4621 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
4681 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
4741 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
4801 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
4861 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
4921 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
4981 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
5041 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
5101 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
5161 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
5221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
5281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
5341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
5401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
5461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
5521 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
5581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
5641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
5701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
5761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
5821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
5881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
5941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
6001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
6061 ccgtattacc gccatgcat
//