LOCUS pEB_189-916 7533 bp ds-DNA circular SYN 14-JUN-2007
DEFINITION .
ACCESSION .
VERSION .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_feature 794..812
/label=T7_RNApol_promoter (-17 to +2)
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3629..3668
/label=right part of MCS since BamHI
/ApEinfo_fwdcolor=#db6bff
/ApEinfo_revcolor=#db6bff
misc_feature 811..811
/label=T7_transcription_start (+1)
/ApEinfo_fwdcolor=#ff80ff
/ApEinfo_revcolor=#ff80ff
misc_feature 2855..3658
/label=eGFP
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_feature 497..796
/label=left half of vaccinia thimidine kinase
/ApEinfo_fwdcolor=#003bff
/ApEinfo_revcolor=#003bff
misc_feature 1..63
/label=N-terminus of vaccinia putative membrane protein
/ApEinfo_fwdcolor=#00ff3c
/ApEinfo_revcolor=#00ff3c
misc_feature 20..481
/label=vaccinia virion protein VACWR093
/ApEinfo_fwdcolor=#00ff5e
/ApEinfo_revcolor=#00ff5e
misc_feature 3675..3798
/label=T7 transcription terminator Tphi
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 7119..7119
/label=oriC_from_pUC19
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_feature 3779..3779
/label=T7 transcription terminator Tphi site
/ApEinfo_fwdcolor=#ff10ff
/ApEinfo_revcolor=#ff10ff
misc_feature 867..1418
/label=EMCV_R IRES since poly(C) to -7 (gataatATG missing)
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
misc_feature 5500..6360
/label=beta lactamase
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=green
misc_feature 1425..2084
/label=CAT
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_feature 2100..2854
/label=ERBV
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=cyan
ORIGIN
1 AAGCTTTTGC GATCAATAAA TGGATCACAA CCAGTATCTC TTAACGATGT TCTTCGCAGA
61 TGATGATTCA TTTTTTAAGT ATTTGGCTAG TCAAGATGAT GAATCTTCAT TATCTGATAT
121 ATTGCAAATC ACTCAATATC TAGACTTTCT GTTATTATTA TTGATCCAAT CAAAAAATAA
181 ATTAGAAGCC GTGGGTCATT GTTATGAATC TCTTTCAGAG GAATACAGAC AATTGACAAA
241 ATTCACAGAC TTTCAAGATT TTAAAAAACT GTTTAACAAG GTCCCTATTG TTACAGATGG
301 AAGGGTCAAA CTTAATAAAG GATATTTGTT CGACTTTGTG ATTAGTTTGA TGCGATTCAA
361 AAAAGAATCC TCTCTAGCTA CCACCGCAAT AGATCCTGTT AGATACATAG ATCCTCGTCG
421 CAATATCGCA TTTTCTAACG TGATGGATAT ATTAAAGTCG AATAAAGTGA ACAATAATTA
481 ATTCTTTATT GTCATCATGA ACGGCGGACA TATTCAGTTG ATAATCGGCC CCATGTTTTC
541 AGGTAAAAGT ACAGAATTAA TTAGACGAGT TAGACGTTAT CAAATAGCTC AATATAAATG
601 CGTGACTATA AAATATTCTA ACGATAATAG ATACGGAACG GGACTATGGA CGCATGATAA
661 GAATAATTTT GAAGCATTGG AAGCAACTAA ACTATGTGAT GTCTTGGAAT CAATTACAGA
721 TTTCTCCGTG ATAGGTATCG ATGAAGGACA GTTCTTTCCA GACATTGTTG AATTGATCTC
781 GATCCCGCGA AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGCGGG
841 ATCAATTCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT
901 AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG
961 TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC
1021 TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT
1081 CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG
1141 ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC
1201 CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG
1261 TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG
1321 GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC
1381 GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATAA TACCATGGAG AAAAAAATCA
1441 CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC
1501 AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA
1561 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC
1621 TGATGAATGC TCATCCGGAA CTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG
1681 ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT
1741 GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT
1801 GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT
1861 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT
1921 TCTTCGCCCC CGTTTTCACG ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC
1981 CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA
2041 ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGC GCTACCGGTA
2101 TTGATGTGTT GGTCGTTTGC CAATCGGAGG GCGACAGGTC GTTTGCCAAT CGGAGGGCGA
2161 CAGGCACAGG TCGCTCCGAG TTCTAGTAGT GTGGGAACTT GTTACTACTG ATGAAACGAG
2221 GTAGTGACAC TGACTACCTG CGAACGAGGT CGGGGCCCTC CCTTCTTCCT TCACCCAACT
2281 TTCACTTTTC GTTCCACTTT AGCAGGGGTC TTCTTTCTAT CCCCCTGGCG GCATTGGAAC
2341 TAGCCGTCGC GTCTTAACGC GCAGCCCTGA AGGCCCCACA CCTTGTGGAT CTTGCCGTGG
2401 GTATGTTTCT GGCATGTGTT TCTCAAGCCT GCAACCGAAG CCGAACAGCC ACATGAACAG
2461 TTTGAGCGTG GTAGCGCTGT GTGAGTTGGC GGTGGATCCC CCTCGTGGTA ACACGAGCCC
2521 CCGTGGCCAA AAGCCCAGTG TTTACAGCAC CTCTCACATC CAGGACGACC CCATCCTGGC
2581 GCTCACTCTT AGTAGTATGG CTTAGTACGC ATTAGGTGGT AAGCCGAGCT CTCCCTCGGC
2641 CTTGTTCTGA ATGCACACAT GTCTAGGGGC TAAGGATGTC CTACAGGTAC CCGCACGTAA
2701 CCTTCAGAGA GTGCGGATCT GAGTAGGAGA CCGTGGTGCA CTGCTTTACA GATGCAGCCC
2761 CGGTTTAAAA AGCGTCTATG CCCCTACAGG GTAGCGGTGG GCCGCGCCCT TTCCTTTTAA
2821 AACTACTTGT TCTATGGTGA CAATGGCAGG AAACATGGTG AGCAAGGGCG AGGAGCTGTT
2881 CACCGGGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
2941 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
3001 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
3061 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
3121 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
3181 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
3241 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
3301 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
3361 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
3421 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
3481 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
3541 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCTCGAGCTC AAGCTTCGAA
3601 TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCCTGCAG CTCGAGAGGC CTAATTAATT
3661 AAGTCGACGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
3721 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
3781 TGAAAGGAGG AACTATATCC GGATCGAGAT CAATTCTGTG AGCGTATGGC AAACGAAGGA
3841 AAAATAGTTA TAGTAGCCGC ACTCGATGGG ACATTTCAAC GTAAACCGTT TAATAATATT
3901 TTGAATCTTA TTCCATTATC TGAAATGGTG GTAAAACTAA CTGCTGTGTG TATGAAATGC
3961 TTTAAGGAGG CTTCCTTTTC TAAACGATTG GGTGAGGAAA CCGAGATAGA AATAATAGGA
4021 GGTAATGATA TGTATCAATC GGTGTGTAGA AAGTGTTACA TCGACTCATA ATATTATATT
4081 TTTTATCTAA AAAACTAAAA ATAAACATTG CTTAAATTTT AATATAATAC TTAAAAATGG
4141 ATGTTGTGTC GTTAGATAAA CCGTTTATGT ATTTTGAGGA AATTGATAAT GAGTTAGATT
4201 ACGAACCAGA AAGTGCAAAT GAGGTCGCAA AAAAACTGCC GTATCAAGGA CAGTTAAAAC
4261 TATTACTAGG AGAATTATTT TTTCTTAGTA AGTTACAGCG ACACGGTATA TTAGATGGTG
4321 CCACCGTAGT GTATATAGGA TCTGCTCCCG GTACACATAT ACGTTATTTG AGAGATCATT
4381 TCTATAATTT AGGAGTGATC ATCAAATGGA TGCTAATTGA CGGCCGCCAT CATGATCCTA
4441 TTTTAAATGG ATTGCGTGAT GTGACTCTAG TGACTCGGTT CGTTGATGAG GAATATCTAC
4501 GATCCATCAA AAAACAACTG CATCCTTCTA AGATTATTTT AATTTCTGAT GTGAGATCCA
4561 AACGAGGAGG AAATGAACCT AGTACGGCGG ATTTACTAAG TAATTACGCT CTACAAAATG
4621 TCATGATTAG TATTTTAAAC CCCGTGGCGT CTAGTCTTAA ATGGAGATGC CCGTTTCCAG
4681 ATCAATGGAT CAAGGACTTT TATATCCCAC ACGGTAATAA AATGTTACAA CCTTTTGCTC
4741 CTTCATATTC AGGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
4801 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
4861 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCG ACGCGCCCTG
4921 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC
4981 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG
5041 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG
5101 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG
5161 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT
5221 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT
5281 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT
5341 TAACAAAATA TTAACGTTTA CAATTTCCCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA
5401 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA
5461 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT
5521 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTC TTTTTGCTCA CCCAGAAAGC
5581 GTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG
5641 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG
5701 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG
5761 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA
5821 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG
5881 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC
5941 GCTTTTTTGC ACAACATGGG GGATCATCTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG
6001 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG
6061 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC
6121 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG
6181 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG
6241 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT
6301 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA
6361 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT
6421 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG
6481 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
6541 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
6601 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
6661 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
6721 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
6781 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
6841 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
6901 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
6961 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
7021 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
7081 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
7141 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
7201 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
7261 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG
7321 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG
7381 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG
7441 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT
7501 CACACAGGAA ACAGCTATGA CCATGATTAC GCC
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