LOCUS pLXRN 6094 bp ds-DNA linear 12-JUN-2008 DEFINITION Cloning vector pLXRN, complete sequence. ACCESSION AF113968 VERSION AF113968.2 GI:13621323 KEYWORDS . SOURCE Cloning vector pLXRN ORGANISM Cloning vector pLXRN other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6094) AUTHORS Mathupala,S.P. TITLE Bicistronic retroviral vector pLXRN JOURNAL Unpublished REFERENCE 2 (bases 1 to 6094) AUTHORS Mathupala,S.P. TITLE Direct Submission JOURNAL Submitted (15-DEC-1998) Biological Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA REFERENCE 3 (bases 1 to 6094) AUTHORS Mathupala,S.P. TITLE Direct Submission JOURNAL Submitted (13-APR-2001) Biological Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA REMARK Sequence update by submitter COMMENT On Apr 13, 2001 this sequence version replaced gi:4416095. COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 2..3801 /organism="Cloning vector pLXRN" /mol_type="genomic DNA" /db_xref="taxon:89525" /note="constructed by replacing the SV40 promoter of vector pLXSN (Genbank Accession Number M28248) with the internal ribosome entry site of encephalomyocarditis virus; the four original restriction sites of the pLXSN vector, EcoRI, HpaI, XhoI, and BamHI were retained during the cloning procedure" /label=constructed by replacing the SV40 promoter of vect /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink source 3802..6094 /organism="Cloning vector pBR322" /mol_type="other DNA" /db_xref="taxon:47470" /tissue_lib="ATCC 31344, ATCC 37017" /focus /label=source:Cloning vector pBR322 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink LTR 175..763 /note="5' MoMSV LTR" /label=5' Mo-MLV LTR /ApEinfo_fwdcolor=#ff00cb /ApEinfo_revcolor=#ff00cb misc_signal 833..1642 /note="packaging signal" /label=packaging signal /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 1644..1665 /note="multiple cloning site: EcoRI, HpaI, XhoI, and BamHI" /label=multiple cloning site: EcoRI, HpaI, XhoI, and BamH /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=cyan CDS 2277..3080 /gene="Neo" /function="eukaryotic neomycin resistance" /function="eukaryotic G-418 resistance" /codon_start=1 /product="neomycin phosphotransferase" /protein_id="AAD20228.1" /db_xref="GI:4416096" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 LTR 3150..3733 /note="3' MoMLV LTR" /label=3' MoMLV LTR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 1667..2253 /label=EMCV-R IRES /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff CDS 3802..3842 /codon_start=1 /transl_table=11 /product="ROP protein" /protein_id="AAB59736.1" /db_xref="GI:456436" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH ADELYRSCLARFGDDGENL" /label=ROP protein /ApEinfo_fwdcolor=#ffff9e /ApEinfo_revcolor=#ffff9e misc_feature 3802..3903 /note="H-strand Y effector site" /citation=[5] /label=H-strand Y effector site /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff repeat_unit complement(3981..3985) /note="gamma-delta insertion target sequence" /rpt_type=direct /label=gamma-delta insertion target sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(4087..4150) /note="L-strand Y effector site" /citation=[5] /label=L-strand Y effector site /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_binding 4175..4183 /bound_moiety="dnaA" /label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 4271..4271 /label=rep_origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb old_sequence 4465..4466 /note="revision according to [17]" /citation=[2] /citation=[17] /replace="at" /label=revision according to [17] /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink old_sequence 4465..4465 /citation=[17] /label=old_sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink old_sequence 4466..4466 /citation=[17] /label=old_sequence(1) /ApEinfo_label=old_sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink old_sequence 4466..4466 /note="revision according to [16]" /citation=[2] /citation=[16] /replace="t" /label=revision according to [16] /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink repeat_region 4884..6094 /mobile_element="transposon:Tn3" /label=repeat_region /ApEinfo_fwdcolor=#89c0cb /ApEinfo_revcolor=#89c0cb repeat_region 4884..4921 /note="corresponds to one of the 38bp repeats found in Tn3 (bp 1-38 and complement (4920-4957))" /rpt_type=inverted /label=corresponds to one of the 38bp repeats found in Tn /ApEinfo_fwdcolor=#89c0cb /ApEinfo_revcolor=#89c0cb sig_peptide complement(5821..5889) /gene="bla" /label=bla /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink gene complement(5029..5889) /gene="bla" /label=bla(1) /ApEinfo_label=bla /ApEinfo_fwdcolor=#00ff6e /ApEinfo_revcolor=#00ff6e mat_peptide complement(5032..5820) /gene="bla" /product="beta-lactamase" /label=beta-lactamase /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink RBS complement(5897..5901) /note="Shine-Dalgarno sequence" /label=Shine-Dalgarno sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6004..6007) /bound_moiety="echinomycin" /label=misc_binding(1) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6016..6019) /bound_moiety="echinomycin" /label=misc_binding(2) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6021..6024) /bound_moiety="echinomycin" /label=misc_binding(3) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6032..6035) /bound_moiety="echinomycin" /label=misc_binding(4) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6047..6050) /bound_moiety="echinomycin" /label=misc_binding(5) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6053..6056) /bound_moiety="echinomycin" /label=misc_binding(6) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(6067..6070) /bound_moiety="echinomycin" /label=misc_binding(7) /ApEinfo_label=misc_binding /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter complement(5924..5930) /note="promoter P3 [6]" /label=promoter P3 [6] /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS complement(5029..5889) /gene="bla" /note="E-286" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAB59737.1" /db_xref="GI:455370" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /label=beta-lactamase(1) /ApEinfo_label=beta-lactamase /ApEinfo_fwdcolor=#ffff9e /ApEinfo_revcolor=#ffff9e misc_feature 3589..3656 /label=R /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3140..3588 /label=U3 /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff misc_feature 883..950 /label=Mo-MuLV promoter region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 951..1025 /label=roughly 5'-UTR of Mo-MuLV /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 951..951 /label=Mo-MuLV transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff ORIGIN 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccgcccc tctccctccc 1681 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgtg tttgtctata 1741 tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 1801 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 1861 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 1921 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 1981 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 2041 tagttgtgga aagagtcaaa tggctctcct caagcgtagt caacaagggg ctgaaggatg 2101 cccagaaggt accccattgt atgggaatct gatctggggc ctcggtgcac atgctttaca 2161 tgtgtttagt cgaggttaaa aaagctctag gccccccgaa ccacggggac gtggttttcc 2221 tttgaaaaac acgatgataa gcttgccaca accccgggat aattcctgca gccaatatgg 2281 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 2341 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 2401 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 2461 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 2521 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 2581 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 2641 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 2701 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 2761 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 2821 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 2881 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 2941 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 3001 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 3061 ttcttgacga gttcttctga gcgggactct ggggttcgat aaaataaaag attttattta 3121 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag 3181 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa 3241 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 3301 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat 3361 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt 3421 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga 3481 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg 3541 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc 3601 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg 3661 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg 3721 ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac 3781 ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 3841 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 3901 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 3961 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 4021 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 4081 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4681 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5341 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5641 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6001 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 6061 aaataggcgt atcacgaggc cctttcgtct tcaa //