LOCUS p_cmvbGalCAT 9877 bp ds-DNA circular 08-JUL-2008 DEFINITION bicistronic vector pBgal/CAT with deleted CMV promoter. SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 4359..4406 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4407..5250 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T(1) /ApEinfo_label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_binding complement(7534..7556) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 7343..7350 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 130..3273 /label=LacZ /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(7896..8578) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS 307..375 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 5552..5858 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 6374..7168 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 3610..4269 /label=CAT /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=green polyA_signal 5289..5520 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink polyA_signal complement(5014..5205) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 6061..6386 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(5270..5287) /vntifkey="21" /label=SP6 /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#ffff80 misc_feature 8673..9533 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5222..5239) /label=SP6(1) /ApEinfo_label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7391..7521 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA(1) /ApEinfo_label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink primer_bind complement(7508..7528) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attctgcaga 61 tatccatcac actggcggcc gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa 121 gaagtcacca tgtcgtttac tttgaccaac aagaacgtga ttttcgttgc cggtctggga 181 ggcattggtc tggacaccag caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt 241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 361 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 421 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 481 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt 541 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 601 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 661 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 721 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac 781 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 841 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 901 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 961 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 1021 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 1081 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 1141 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1201 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1261 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1321 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1381 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1441 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1501 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1561 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1621 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1681 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1741 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1801 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1861 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1921 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1981 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 2041 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 2101 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 2161 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2221 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2281 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2341 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2401 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2461 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2521 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2581 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2641 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2701 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2761 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2821 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2881 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2941 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 3001 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 3061 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 3121 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3181 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc 3241 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc 3301 gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc 3361 ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt tttcccgatt 3421 tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt tgccgctatt 3481 tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcggcctc 3541 gactctaggc ggccgctcga gctagaggat ctgagcttgg cgagattttc aggagctaag 3601 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat 3661 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt 3721 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 3781 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg 3841 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag 3901 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta 3961 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 4021 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 4081 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat 4141 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat 4201 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 4261 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa 4321 taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac 4381 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 4441 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 4501 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 4561 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 4621 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 4681 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 4741 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc 4801 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg 4861 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt 4921 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag 4981 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 5041 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 5101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgcctag agggccctat 5221 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 5281 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 5341 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 5401 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 5461 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 5521 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 5581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 5641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt 5701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 5761 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 5821 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 5881 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat 5941 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 6001 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 6061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 6121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 6181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6241 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 6301 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 6361 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 6421 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 6481 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 6541 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 6601 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 6661 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 6721 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 6781 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 6841 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 6901 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 6961 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 7021 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 7081 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 7141 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 7201 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 7261 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 7321 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 7381 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7441 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 7501 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7561 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7621 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7681 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8101 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 9001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9661 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 9721 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 9781 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 9841 agaatctgct tagggttagg cgttttgcgc tgcttcg //