LOCUS pUtrACAT 7548 bp ds-DNA circular 08-JUL-2008 DEFINITION monicistronic utrophin A reporter construct. SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 6840..7494 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 2203..2434 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink promoter 7495..7513 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 2975..3300 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink 5'UTR 12..517 /vntifkey="52" /label=utrophin\A\IRES /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(2136..2153) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 7446..7465 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 2466..2772 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 524..1183 /label=CAT /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=green misc_feature 7463..7463 /label=T7 transcription start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 3288..4082 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7414..7414 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1321..2164 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature complement(2184..2201) /vntifkey="21" /label=SP6(1) /ApEinfo_label=SP6 /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#ffff80 polyA_signal complement(1928..2119) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4305..4435 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink primer_bind complement(4422..4442) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(4810..5492) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_binding complement(4448..4470) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5587..6447 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 ggccgctcga gttgtggagt cgcccttccc ccgaggaggg gagcgggcag cgccgggcgc 61 gggatttctc ccgcccaggg ccgaggagga gccctgggct cctcaaccga tcgggccacc 121 tccgggagcg gcgccccttt tctttcgggt cattcctgca gagggaaaac tcctgcgtgc 181 gcctgccccc aggctagcgt ttggactgtt ttgtttttgg cggaactacc gggcaggaag 241 attgcacaag tcagggggcg tttccaatcg ggtgtcaatt ttggatctcc ccccccccac 301 ccccctttgg atcttgtcgg gctttccacg tttcacttaa aaaaaattct tgtaacgtcc 361 aaggcaagct ccccacgacc cgaacacgcc cagaggaacc ttggggcctc tggggacttg 421 gctagcaggt attcatgcta gcctggacca tttttcagat ttagccttca gaagaggatc 481 tgggaaagcc tttggattat actgaaactc attcaagctc gagatggaga aaaaaatcac 541 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca 601 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa 661 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct 721 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga 781 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg 841 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg 901 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc 961 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt 1021 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc 1081 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa 1141 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta 1201 ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc 1261 agaaattcgc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa 1321 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt 1381 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg 1441 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc 1501 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa 1561 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt 1621 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct 1681 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa 1741 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta 1801 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt 1861 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac 1921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 1981 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 2041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 2101 atgtatctta tcatgtctgc ctagagggcc ctattctata gtgtcaccta aatgctagag 2161 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 2221 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 2281 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 2341 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 2401 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 2461 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 2521 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 2581 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 2641 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 2701 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 2761 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 2821 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 2881 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa 2941 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 3001 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 3061 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 3121 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 3181 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 3241 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 3301 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 3361 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 3421 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 3481 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 3541 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 3601 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 3661 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 3721 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 3781 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 3841 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 3901 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 3961 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 4021 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 4081 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 4141 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 4201 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 4261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 4321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 4381 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 4441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 4501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 4561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 4621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 4741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 4801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 4861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 4921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 4981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 5041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 5161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 5221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 5281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 5341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 5401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 5581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 5641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 5701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 5761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 5821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 5941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 6061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 6121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 6181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 6241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 6301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 6361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 6601 cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag 6661 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 6721 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt 6781 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat 6841 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 6901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 6961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 7021 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 7081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 7141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 7201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 7261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 7321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 7381 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc 7441 gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg gatccactag 7501 taacggccgc cagtgtgctg gaattctgca gatatccatc acactggc //