LOCUS pCAT_Iresite 7037 bp ds-DNA linear 26-SEP-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >pCAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1809..2655 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink promoter 3463..3788 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 3824..4618 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(2672..2689) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4793..4923 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 6123..6983 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal 2691..2922 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink rep_origin complement(5346..6028) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 polyA_signal complement(2464..2655) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(4958..4978) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 3002..3308 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_binding complement(4984..5006) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 1060..1719 /label=CAT /ApEinfo_fwdcolor=#004080 /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgaga tggagaaaaa aatcactgga 1081 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 1141 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 1201 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 1261 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 1321 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 1381 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 1441 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 1501 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 1561 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 1621 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa 1681 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg 1741 tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa 1801 attcgccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta 1861 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa 1921 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag 1981 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt 2041 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta 2101 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt 2161 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac 2221 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat 2281 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct 2341 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg 2401 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc 2461 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 2521 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 2581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 2641 atcttatcat gtctgcctag agggccctat tctatagtgt cacctaaatg ctagagctcg 2701 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2761 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2821 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2881 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2941 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 3001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 3061 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 3121 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct 3181 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 3241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 3301 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat 3361 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 3421 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 3481 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3601 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3661 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3721 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3781 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3841 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3901 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3961 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 4021 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4081 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4141 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4201 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4261 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4321 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4381 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4441 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4501 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4561 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4621 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4681 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4741 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 4801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4921 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 4981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 5041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 5101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 5161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 7021 ggttattgtc tcatgag //