LOCUS GAL_620_1222_CAT_I 11120 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/620-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5892..6738 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6547..6738) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6774..7005 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6755..6772) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(9429..10111) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 10206..11066 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7546..7871 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(9041..9061) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7907..8701 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(9067..9089) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 7085..7391 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8876..9006 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..5136 /label=Hiap2_620-1222 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 5143..5802 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gagaattact tttgtttatt ggaataaaat 4561 gagatttttt gggttgtcat gttaaagtgc ttatagggaa agaagcctgc atataatttt 4621 ttaccttgtg gcataatcag taattggtct gttattcagg cttcatagct tgtaaccaaa 4681 tataaataaa aggcataatt taggtattct atagttgctt agaattttgt taatataaat 4741 ctctgtgaaa aatcaaggag ttttaatatt ttcagaagtg catccacctt tcagggcttt 4801 aagttagtat tactcaagat tatgaacaaa tagcacttag gttacctgaa agagttacta 4861 caaccccaaa gagttgtgtt ctaagtagta tcttggtaat tcagagagat actcatccta 4921 cctgaatata aactgagata aatccagtaa agaaagtgta gtaaattcta cataagagtc 4981 tatcattgat ttctttttgt ggtaaaaatc ttagttcatg tgaagaaatt tcatgtgaat 5041 gttttagcta tcaaacagta ctgtcaccta ctcatgcaca aaactgcctc ccaaagactt 5101 ttcccaggtc cctcgtatca aaacattaag agtatactcg agatggagaa aaaaatcact 5161 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 5221 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 5281 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 5341 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 5401 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 5461 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 5521 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 5581 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 5641 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 5701 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat 5761 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat 5821 tggtgccctt aaacgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca 5881 gaaattcgcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa 5941 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt 6001 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg 6061 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct 6121 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag 6181 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt 6241 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta 6301 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat 6361 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat 6421 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg 6481 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact 6541 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 6601 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6721 tgtatcttat catgtctgcc tagagggccc tattctatag tgtcacctaa atgctagagc 6781 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 7021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 7081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 7141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 7201 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 7261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 7321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 7381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 7441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 7501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag 7561 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 7621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 7681 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 7741 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 7801 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 7861 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 7921 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 7981 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 8041 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 8101 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 8161 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 8221 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 8281 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 8341 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 8401 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 8461 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 8521 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 8581 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 8641 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 8701 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 8761 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 8821 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 8881 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 8941 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 9001 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 9061 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 9121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 9181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 9241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 9301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 9361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 9421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 9481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 9541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 9601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 9661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 9721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 9781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 9841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 9901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 9961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 10021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 10081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 10141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 10201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 10261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 10321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 10381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 10441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 10501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 10561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 10621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 10681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 10741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 10801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 10861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 10921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 10981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 11041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 11101 cagggttatt gtctcatgag //