LOCUS GAL_990_1222_CAT_I 10750 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/990-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5522..6368 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6177..6368) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6404..6635 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6385..6402) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(9059..9741) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 9836..10696 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7176..7501 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(8671..8691) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7537..8331 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(8697..8719) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 6715..7021 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8506..8636 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..4766 /label=Hiap2_990-1222 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4773..5432 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gaggagagat actcatccta cctgaatata 4561 aactgagata aatccagtaa agaaagtgta gtaaattcta cataagagtc tatcattgat 4621 ttctttttgt ggtaaaaatc ttagttcatg tgaagaaatt tcatgtgaat gttttagcta 4681 tcaaacagta ctgtcaccta ctcatgcaca aaactgcctc ccaaagactt ttcccaggtc 4741 cctcgtatca aaacattaag agtatactcg agatggagaa aaaaatcact ggatatacca 4801 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 4861 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 4921 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 4981 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 5041 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 5101 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 5161 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 5221 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 5281 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 5341 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 5401 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt 5461 aaacgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc 5521 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag 5581 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga 5641 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt 5701 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg 5761 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc 5821 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa 5881 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa 5941 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac 6001 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat 6061 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg 6121 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 6181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 6241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 6301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6361 catgtctgcc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca 6421 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 6481 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 6541 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 6601 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 6661 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 6721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 6781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 6841 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 6901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 6961 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 7021 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 7081 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 7141 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 7201 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 7261 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7381 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 7441 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 7501 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 7561 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 7621 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 7681 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 7741 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 7801 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 7861 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 7921 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 7981 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 8041 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 8101 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 8161 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 8221 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 8281 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 8341 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 8401 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 8461 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 8521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 8581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 8641 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 8701 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8761 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8821 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 8881 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 8941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 9001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 9061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 9121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9241 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 9301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 9361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 9421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 9541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9781 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 9901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 9961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 10021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 10081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 10141 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 10201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 10261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 10321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 10381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 10441 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 10501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10741 gtctcatgag //