LOCUS GAL_1142_1222_CAT_ 10598 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/1142-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5370..6216 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6025..6216) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6252..6483 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6233..6250) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(8907..9589) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 9684..10544 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7024..7349 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(8519..8539) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7385..8179 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(8545..8567) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 6563..6869 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8354..8484 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..4614 /label=Hiap2_1142-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 4621..5280 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gagcagtact gtcacctact catacacaaa 4561 actgcctccc aaagactttt cccaggtccc tcgtatcaaa acattaagag tatactcgag 4621 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 4681 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 4741 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 4801 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 4861 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 4921 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 4981 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 5041 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 5101 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 5161 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat 5221 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 5281 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata 5341 ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta cttctgtggt 5401 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt 5461 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc 5521 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 5581 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct 5641 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt 5701 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag 5761 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt 5821 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg 5881 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg 5941 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac 6001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 6061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 6121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6181 tttgtccaaa ctcatcaatg tatcttatca tgtctgccta gagggcccta ttctatagtg 6241 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 6301 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 6361 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 6421 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 6481 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 6541 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 6601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 6661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 6721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 6781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 6841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 6901 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 6961 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 7021 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 7081 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 7141 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 7201 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 7261 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 7321 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 7381 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 7441 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 7501 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 7561 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 7621 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 7681 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 7741 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 7801 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 7861 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 7921 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 7981 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 8041 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 8101 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 8161 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 8221 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 8281 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 8341 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8461 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 8521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa 9121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 9661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 9721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 10021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 10081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 10141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 10201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 10261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10561 tattattgaa gcatttatca gggttattgt ctcatgag //