LOCUS _1_1222_CAT_Iresit 8313 bp ds-DNA linear 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >1-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 3085..3931 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(3740..3931) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 3967..4198 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(3948..3965) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(6622..7304) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 7399..8259 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 4739..5064 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(6234..6254) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 5100..5894 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(6260..6282) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 4278..4584 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 6069..6199 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 1060..2281 /label=Hiap2_1-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 2336..2995 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagg aattctatgg agtgtaattt 1081 tgtgtatgaa ttatattttt aaaacattga agagttttca gaaagaaggc tagtagagtt 1141 gattactgat actttatgct aagcagtact tttttggtag tacaatattt tgttaggcgt 1201 ttctgataac actagaaagg acaagtttta tcttgtgata aattgattaa tgtttacaac 1261 atgactgata attatagctg aatagtcctt aaatgatgaa caggttattt agtttttaaa 1321 tgcagtgtaa aaagtgtgct gtggaaattt tatggctaac taagtttatg gagaaaatac 1381 cttcagttga tcaagaataa tagtggtata caaagttagg aagaaagtca acatgatgct 1441 gcaggaaatg gaaacaaata caaatgatat ttaacaaaga tagagtttac agtttttgaa 1501 ctttaagcca aattcatttg acatcaagca ctatagcagg cacaggttca acaaagcttg 1561 tgggtattga cttcccccaa aagttgtcag ctgaagtaat ttagcccact taagtaaata 1621 ctatgatgat aagctgtgtg aacttagctt ttaaatagtg tgaccatatg aaggttttaa 1681 ttacttttgt ttattggaat aaaatgagat tttttgggtt gtcatgttaa agtgcttata 1741 gggaaagaag cctgcatata attttttacc ttgtggcata atcagtaatt ggtctgttat 1801 tcaggcttca tagcttgtaa ccaaatataa ataaaaggca taatttaggt attctatagt 1861 tgcttagaat tttgttaata taaatctctg tgaaaaatca aggagtttta atattttcag 1921 aagtgcatcc acctttcagg gctttaagtt agtattactc aagattatga acaaatagca 1981 cttaggttac ctgaaagagt tactacaacc ccaaagagtt gtgttctaag tagtatcttg 2041 gtaattcaga gagatactca tcctacctga atataaactg agataaatcc agtaaagaaa 2101 gtgtagtaaa ttctacataa gagtctatca ttgatttctt tttgtggtaa aaatcttagt 2161 tcatgtgaag aaatttcatg tgaatgtttt agctatcaaa cagtactgtc acctactcat 2221 gcacaaaact gcctcccaaa gacttttccc aggtccctcg tatcaaaaca ttaagagtat 2281 actcgagcta gaggatctga gcttggcgag attttcagga gctaaggaag ctaaaatgga 2341 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 2401 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 2461 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 2521 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 2581 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 2641 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 2701 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 2761 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 2821 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 2881 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg 2941 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt 3001 ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag 3061 cggatgaatg gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac 3121 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag 3181 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg 3241 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga 3301 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa 3361 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag 3421 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa 3481 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca 3541 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc 3601 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa 3661 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg 3721 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 3781 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 3841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3901 ccaaactcat caatgtatct tatcatgtct gcctagaggg ccctattcta tagtgtcacc 3961 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 4021 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 4081 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 4141 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 4201 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 4261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4381 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 4441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 4501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 4621 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4681 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 4741 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4861 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4921 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 4981 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 5041 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 5101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 5161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 5221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 5281 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 5341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 5401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 5461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 5521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 5581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 5641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 5701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 5761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 5821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 5881 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 5941 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 6001 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 6061 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6181 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 6241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 6841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 7021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 7081 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 7141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 7201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 8041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 8101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 8161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 8221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8281 ttgaagcatt tatcagggtt attgtctcat gag //