LOCUS _497_1222_CAT_Ires 7769 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 497-1222/CAT standard; circular DNA; ; 7769 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493819935| CC VNTDBDATE|493819935| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 2541..3387 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal 3423..3654 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink rep_origin complement(6078..6760) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 promoter 4195..4520 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4556..5350 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 3734..4040 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green polyA_signal complement(3196..3387) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(3404..3421) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 6855..7715 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5690..5710) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(5716..5738) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5525..5655 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 1060..1785 /label=Hiap2_497-1222 /ApEinfo_fwdcolor=#80ff00 /ApEinfo_revcolor=green misc_feature 1792..2451 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagg cttgtgggta ttgacttccc 1081 ccaaaagttg tcagctgaag taatttagcc cacttaagta aatactatga tgataagctg 1141 tgtgaactta gcttttaaat agtgtgacca tatgaaggtt ttaattactt ttgtttattg 1201 gaataaaatg agattttttg ggttgtcatg ttaaagtgct tatagggaaa gaagcctgca 1261 tataattttt taccttgtgg cataatcagt aattggtctg ttattcaggc ttcatagctt 1321 gtaaccaaat ataaataaaa ggcataattt aggtattcta tagttgctta gaattttgtt 1381 aatataaatc tctgtgaaaa atcaaggagt tttaatattt tcagaagtgc atccaccttt 1441 cagggcttta agttagtatt actcaagatt atgaacaaat agcacttagg ttacctgaaa 1501 gagttactac aaccccaaag agttgtgttc taagtagtat cttggtaatt cagagagata 1561 ctcatcctac ctgaatataa actgagataa atccagtaaa gaaagtgtag taaattctac 1621 ataagagtct atcattgatt tctttttgtg gtaaaaatct tagttcatgt gaagaaattt 1681 catgtgaatg ttttagctat caaacagtac tgtcacctac tcatgcacaa aactgcctcc 1741 caaagacttt tcccaggtcc ctcgtatcaa aacattaaga gtatactcga gatggagaaa 1801 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag 1861 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc 1921 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 1981 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg 2041 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 2101 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 2161 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 2221 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 2281 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg 2341 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga 2401 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa 2461 ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga 2521 tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa 2581 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta 2641 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact 2701 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa 2761 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag 2821 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat 2881 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct 2941 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac 3001 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt 3061 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag 3121 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga 3181 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 3241 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3361 actcatcaat gtatcttatc atgtctgcct agagggccct attctatagt gtcacctaaa 3421 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 3481 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 3541 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3601 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3661 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3781 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3841 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc 3901 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 4021 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 4081 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 4141 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc 4201 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 4261 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 4321 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 4381 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 4441 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 4501 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 4561 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 4621 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 4681 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 4741 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 4801 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 4861 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 4921 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 4981 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 5041 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 5101 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 5161 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 5221 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 5281 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 5341 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 5401 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 5461 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 5521 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5641 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 5701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 5761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 5821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 5881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 5941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 6001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 6061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 6301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 6541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 6781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 6841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 6901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 6961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 7021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 7081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 7141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7741 agcatttatc agggttattg tctcatgag //