LOCUS _990_1222_CAT_Ires 7276 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 990-1222/CAT standard; circular DNA; ; 7276 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493819540| CC VNTDBDATE|493819540| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 2048..2894 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal 2930..3161 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink misc_feature 6362..7222 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(5585..6267) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 polyA_signal complement(2703..2894) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 3702..4027 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4063..4857 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 3241..3547 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 primer_bind complement(2911..2928) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5197..5217) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(5223..5245) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5032..5162 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 1060..1292 /label=Hiap2_990-1222 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 1300..1958 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagg agagatactc atcctacctg 1081 aatataaact gagataaatc cagtaaagaa agtgtagtaa attctacata agagtctatc 1141 attgatttct ttttgtggta aaaatcttag ttcatgtgaa gaaatttcat gtgaatgttt 1201 tagctatcaa acagtactgt cacctactca tgcacaaaac tgcctcccaa agacttttcc 1261 caggtccctc gtatcaaaac attaagagta tactcgagat ggagaaaaaa atcactggat 1321 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 1381 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 1441 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 1501 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg 1561 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 1621 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 1681 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 1741 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 1801 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 1861 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat 1921 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt 1981 gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa 2041 ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac 2101 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac 2161 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc 2221 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg 2281 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag 2341 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta 2401 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca 2461 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata 2521 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc 2581 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt 2641 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct 2701 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 2761 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 2821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 2881 tcttatcatg tctgcctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc 2941 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 3001 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 3061 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 3121 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 3181 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 3241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3361 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc 3421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt 3601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 3661 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg 3721 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3841 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3901 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 3961 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 4021 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 4081 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 4141 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 4201 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 4261 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 4321 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 4381 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 4441 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 4501 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 4561 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 4621 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 4681 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 4741 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 4801 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 4861 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 4921 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4981 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5161 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 5221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5761 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 5821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6001 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 6061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 6181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 6241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 6301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 7261 gttattgtct catgag //