LOCUS _1142_1222_CAT_Ire 7124 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 1142-1222/CAT standard; circular DNA; ; 7124 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493819645| CC VNTDBDATE|493819645| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1896..2742 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink promoter 3550..3875 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 3911..4705 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(2759..2776) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4880..5010 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 6210..7070 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal complement(2551..2742) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(5045..5065) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green polyA_signal 2778..3009 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink mutation 1080..1080 /vntifkey="62" /label=ATG-->ATA /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=pink rep_origin complement(5433..6115) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 3089..3395 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_binding complement(5071..5093) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 1060..1140 /label=Hiap2_1142-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1147..1806 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagc agtactgtca cctactcata 1081 cacaaaactg cctcccaaag acttttccca ggtccctcgt atcaaaacat taagagtata 1141 ctcgagatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt 1201 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag 1261 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc 1321 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa 1381 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa 1441 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac 1501 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt 1561 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta 1621 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg 1681 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc 1741 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg 1801 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa 1861 gtgataataa gcggatgaat ggcagaaatt cgccggatct ttgtgaagga accttacttc 1921 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata 1981 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt 2041 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg 2101 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct 2161 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta 2221 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc 2281 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt 2341 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat 2401 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt 2461 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca 2521 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 2581 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 2641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 2701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgcctagagg gccctattct 2761 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 2821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 3001 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 3061 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg 3241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 3301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 3361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 3421 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta 3481 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 3541 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3661 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3781 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3841 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3901 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3961 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 4021 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 4081 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 4141 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 4201 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 4261 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 4321 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 4381 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4441 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4501 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4561 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4621 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4681 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4741 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4801 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4861 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4921 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4981 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 5041 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5101 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5161 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5221 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 5281 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5341 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5401 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5461 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5521 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5641 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5761 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5821 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5881 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5941 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6001 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6061 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6121 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 6361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 6421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 6481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 6541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 6601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 6661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 6721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 6781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 6841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 6901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 6961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgag //