LOCUS GAL_1_990_CAT_Ires 11507 bp ds-DNA linear 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/1-990/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 6279..7125 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6934..7125) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 7161..7392 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(7142..7159) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(9816..10498) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 10593..11453 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7933..8258 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(9428..9448) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 8294..9088 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(9454..9476) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 7472..7778 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 9263..9393 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..5523 /label=Hiap2_1-990 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 5530..6189 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gaggaattct atggagtgta attttgtgta 4561 tgaattatat ttttaaaaca ttgaagagtt ttcagaaaga aggctagtag agttgattac 4621 tgatacttta tgctaagcag tacttttttg gtagtacaat attttgttag gcgtttctga 4681 taacactaga aaggacaagt tttatcttgt gataaattga ttaatgttta caacatgact 4741 gataattata gctgaatagt ccttaaatga tgaacaggtt atttagtttt taaatgcagt 4801 gtaaaaagtg tgctgtggaa attttatggc taactaagtt tatggagaaa ataccttcag 4861 ttgatcaaga ataatagtgg tatacaaagt taggaagaaa gtcaacatga tgctgcagga 4921 aatggaaaca aatacaaatg atatttaaca aagatagagt ttacagtttt tgaactttaa 4981 gccaaattca tttgacatca agcactatag caggcacagg ttcaacaaag cttgtgggta 5041 ttgacttccc ccaaaagttg tcagctgaag taatttagcc cacttaagta aatactatga 5101 tgataagctg tgtgaactta gcttttaaat agtgtgacca tatgaaggtt ttaattactt 5161 ttgtttattg gaataaaatg agattttttg ggttgtcatg ttaaagtgct tatagggaaa 5221 gaagcctgca tataattttt taccttgtgg cataatcagt aattggtctg ttattcaggc 5281 ttcatagctt gtaaccaaat ataaataaaa ggcataattt aggtattcta tagttgctta 5341 gaattttgtt aatataaatc tctgtgaaaa atcaaggagt tttaatattt tcagaagtgc 5401 atccaccttt cagggcttta agttagtatt actcaagatt atgaacaaat agcacttagg 5461 ttacctgaaa gagttactac aaccccaaag agttgtgttc taagtagtat cttggtaatt 5521 cagctcgaga tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat 5581 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt 5641 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 5701 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg 5761 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag 5821 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta 5881 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 5941 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 6001 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat 6061 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat 6121 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 6181 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa 6241 taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac 6301 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 6361 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 6421 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 6481 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 6541 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 6601 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 6661 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc 6721 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg 6781 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt 6841 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag 6901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 6961 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 7021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgcctag agggccctat 7141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 7201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 7261 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 7321 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 7381 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 7441 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 7501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 7561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt 7621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 7681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 7741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 7801 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat 7861 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 7921 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 7981 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 8041 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 8101 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 8161 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 8221 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 8281 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 8341 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 8401 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 8461 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 8521 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 8581 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 8641 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 8701 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 8761 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 8821 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 8881 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 8941 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 9001 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 9061 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 9121 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 9181 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 9241 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 9301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 9361 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 9421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 9481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 9541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 9601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 9661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 9721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 9781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 9841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 9901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 9961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 10021 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 10081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 10141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 10201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 10261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 10321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 10381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 10441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 10501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 10561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 10621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 10681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 10741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 10801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 10861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 10921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 10981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 11041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 11101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 11161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 11221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 11281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 11341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 11401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 11461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgag //