LOCUS pCITE2a 3800 bp ds-DNA circular 20-DEC-2008 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 3784..3800 /vntifkey="30" /label=T7 promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 enhancer 16..518 /vntifkey="9" /label=CITE /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink protein_bind 589..606 /vntifkey="31" /label=His tag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter complement(664..680) /vntifkey="30" /label=T3 promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 rep_origin complement(1104..1104) /vntifkey="33" /label=origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb CDS complement(1865..2722) /vntifkey="4" /label=bla (amp) sequence /ApEinfo_fwdcolor=#d3ff00 /ApEinfo_revcolor=#d3ff00 rep_origin complement(3161..3616) /vntifkey="33" /label=f1 origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 17..516 /label=EMCV IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 1..1 /label=T7 promoter transcription start site (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature complement(663..663) /label=T3 transcription site (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 609..614 /label=BglII site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 622..627 /label=XbaI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 638..643 /label=PstI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 644..649 /label=SphI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 583..588 /label=XhoI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 684..710 /label=end of SV40 poly(A) signal /ApEinfo_fwdcolor=#ff7cff /ApEinfo_revcolor=#ff7cff misc_feature 519..626 /label=fusion protein /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 517..522 /label=NcoI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 543..548 /label=BamHI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 555..560 /label=SacI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 562..567 /label=SalI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 ORIGIN 1 gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 61 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg 481 ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggccaccacc catatggata 541 tcggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac 601 caccactgag atctccgatc ctctagagtc gatcgacctg caggcatgca agcttttgtt 661 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt 721 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 781 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 841 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 901 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 1021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 1081 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 1141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 1201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 1261 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 1321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 1381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 1441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 1501 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 1561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 1621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 1681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 1741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 1801 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 1861 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 1921 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 1981 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 2041 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 2101 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 2161 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 2221 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 2281 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 2341 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 2401 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 2461 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 2521 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 2581 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 2641 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 2701 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 2761 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 2821 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 2881 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 2941 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 3001 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 3061 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga 3121 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 3181 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 3241 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 3301 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 3361 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 3421 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 3481 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 3541 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 3601 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 3661 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 3721 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa 3781 ttgtaatacg actcactata //