LOCUS pIRES_lacZ 6819 bp ds-DNA circular 10-APR-2009 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin complement(6361..6816) /vntifkey="33" /note="phage f1 origin" /label=f1\ori /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb promoter complement(6178..6194) /vntifkey="30" /note="phage T7 gene 10 promoter" /label=T7\promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 2859..5918 /vntifkey="4" /note="E. coli lacZ coding sequence" /label=lacZ /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 RBS 2258..2845 /vntifkey="32" /note="internal ribosome entry site of the encephalomyocarditis virus polyprotein (EMCPOLYP) gene" /label=5'\UTR\of\EMC-POLYP\(IRES) /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 2171..2187 /vntifkey="30" /note="phage T3 promoter" /label=T3\promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 promoter 2024..2107 /vntifkey="30" /note="E. coli lac operon promoter" /label=lac\promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 rep_origin 1530..1932 /vntifkey="33" /note="origin plasmid" /label=pMB1\ori /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb CDS 129..989 /vntifkey="4" /note="antibiotic resistance gene; coding sequence" /label=ampicillin /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 2188..2188 /label=T3 transcription start site (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature complement(6177..6177) /label=T7 promoter transcription start site (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg 2221 gccgctctag aactagtgga tcctctagag aattccgccc cccccccccc cccccctctc 2281 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 2341 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 2401 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 2461 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 2521 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 2581 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 2641 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 2701 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 2761 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt 2821 tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac 2881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 2941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 3001 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa 3061 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga 3121 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc 3181 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa 3241 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc 3301 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg 3361 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga 3421 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg 3481 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta 3541 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg 3601 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg 3661 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac 3721 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg 3781 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt 3841 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc 3901 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga 3961 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt 4021 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg 4081 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct 4141 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga 4201 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt 4261 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg 4321 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct 4381 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc 4441 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat 4501 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg 4561 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt 4621 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca 4681 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat 4741 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc 4801 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg 4861 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg 4921 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg 4981 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg 5041 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg 5101 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg 5161 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg 5221 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg 5281 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg 5341 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca 5401 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag 5461 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag 5521 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg 5581 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc 5641 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc 5701 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc 5761 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg 5821 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattacag ctgagcgccg 5881 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccggca ggccatgtct 5941 gcccgtattc gcgtaagaaa tccattatgt actatttggg cgagctcgaa ttggctagag 6001 tcgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6121 nnnnnnnnnn nnnnnnnnnn nnnnnnctcg agggggggcc cggtacccaa ttcgccctat 6181 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac 6241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 6301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 6361 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 6421 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 6481 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 6541 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 6601 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 6661 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 6721 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 6781 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcag //