LOCUS pRst-LEF1_12-F 6700 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 698..1654 /label=RLuc /ApEinfo_fwdcolor=#00ff06 /ApEinfo_revcolor=#00ff06 misc_feature 337..409 /label=SV40_promoter_and_enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 11..253 /label=SV40_promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 669..688 /label=T7_promoter /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff misc_feature 687..687 /label=T7_transcription_start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 493..625 /label=chimeric_intron /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 254..336 /label=SV40_promoter(1) /ApEinfo_label=SV40_promoter /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 4638..4859 /label=SV40_late_polyA /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 325..374 /label=Putative_major_transcription_start_sites /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 1660..1696 /label=Multiple_cloning_site /ApEinfo_fwdcolor=#ff5b00 /ApEinfo_revcolor=#ff5b00 misc_feature 1762..1767 /label=HindIII /ApEinfo_fwdcolor=#ff9b86 /ApEinfo_revcolor=#ff9b86 misc_feature 410..421 /label=SV40_promoter_enhancer /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 2955..2955 /label=NcoI /ApEinfo_fwdcolor=#e6b83e /ApEinfo_revcolor=#e6b83e misc_feature 1662..1767 /label=hairpin /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 1769..1773 /label=BglII_cleaved /ApEinfo_fwdcolor=#ee9e66 /ApEinfo_revcolor=#ee9e66 misc_feature 1656..1656 /label=BglII_cleaved(1) /ApEinfo_label=BglII_cleaved /ApEinfo_fwdcolor=#ec9c4b /ApEinfo_revcolor=#ec9c4b misc_feature 2955..2957 /label=AfeI-cleaved-right_part /ApEinfo_fwdcolor=#ecc376 /ApEinfo_revcolor=#ecc376 misc_feature 1657..1661 /label=BamHI-cleaved_right /ApEinfo_fwdcolor=#dba96b /ApEinfo_revcolor=#dba96b misc_feature 4618..4623 /label=XbaI /ApEinfo_fwdcolor=#e1d8db /ApEinfo_revcolor=#e1d8db misc_feature 1768..1768 /label=BamHI-cleaved_left /ApEinfo_fwdcolor=#dba370 /ApEinfo_revcolor=#dba370 misc_feature 2964..4616 /label=fluc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 2958..2963 /label=BamHI /ApEinfo_fwdcolor=#d9c3be /ApEinfo_revcolor=#d9c3be misc_feature 2351..2738 /label=LEF1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 1777..2182 /label=LEF1(1) /ApEinfo_label=LEF1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 2829..2954 /label=LEF1(2) /ApEinfo_label=LEF1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 2240..2331 /label=LEF1(3) /ApEinfo_label=LEF1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 1777..1800 /label=FWD-primer_5b_685 /ApEinfo_fwdcolor=#ceffff /ApEinfo_revcolor=#ceffff misc_feature 2931..2954 /label=REV-primer_5b_1863 /ApEinfo_fwdcolor=#d6e4ff /ApEinfo_revcolor=#d6e4ff ORIGIN 1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt 61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga 481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg 541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac 601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc 661 tagagtacTT AATACGACTC ACTATAGGct agccaccatG acttcgaaag tttatgatcc 721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa 781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat 841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg 961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct 1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca 1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga 1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc 1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga 1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg 1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga 1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca 1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa 1621 aaatgaacaa atGtcgaTCG ACgggggccc ctaggAgatc cAAGCTTATC GATTTCGAAC 1681 CCGGGGTACC GAATTCCTCG AGTCTAGAGG AgcatgcTCC TCTAGACTCG AGGAATTCGG 1741 TACCCCGGGT TCGAAATCGA TAAGCTTgGA TCTAGCGCGC GGGAGGAGGA GAAGCAGTGG 1801 GGAGGCGCAG CCGCTCAcCT GCGGGGCAGG GCGCGGAGGA GGGACCCGGN TGcGcGCTCT 1861 CGGGCCGAGG AACCAGGACG cGCCCGGAGC CTCGCACGCG GCCAAGCTCG GGCGTCCCCT 1921 CCCCTCGGCC GGGCGAACTC AAGGGGCGCA GCTCTTTGCT TTGACAGAGC TGGCCGGCGG 1981 AGGCGTGCAG AGCGGCGAGC CGGCGAGCCA GGCTGAGAAA CTCGACCCGG GAACAAAGAG 2041 GGGTCGGACT GAGTGTGTGT GTCGGCTCGA GCTCCGGGNA GAGGCATTTG CCCGAGGNCC 2101 CCGCTGTGAC TCCCCGAGAC TCCGCAGTGC CCTCCACTGN GGAGTCCCCG CGCTTGCCGG 2161 NAAAAACTTT ATTCTTGGCA AACTTCTCTT TCTCTTCCCC TCCTCCTCGG CCCCCATCTT 2221 CTGCTCCTCC TCCTTCTCTA GCAGATTAAA TGAGCCTCGA GAAGAAAAAC CGAAGCGAAA 2281 GGGAAGAAAA TAAGAAGATC TAAAACGGAC ATCTCCAGCG TGGGTGGCTC CTTTTTCTTT 2341 TTCTTTTTTT CCCACCCTTC AGGAAGTGGA CGTTTCGTTA TCTTCTGATC CTTGCACCTT 2401 CTTTTGGGGC AAACGGGGCC CTTCTGCCCA GATCCCCTCT CTTTTCTCGG AAAACAAACT 2461 ACTAAGTCGG CATCCGGGGT AACTACAGTG GAGAGGGTTT CCGCGGAGAC GCGCCGCCGG 2521 ACCCTCCTCT GCACTTTGGG GAGGCGTGCT CCCTCCAGAA CCGGCGTTCT CCGCGCGCAA 2581 ATCCCGGCGA CGCGGGGTCG CGGGGTGGCC GCCGGGGCAG CCTCGTCTAG CGCGCGCCGC 2641 GCAGACGCCC CCGGAGTCGC CAGCTACCGC AGCCCTCGCC GCCCAGTGCC CTTCGGCCTC 2701 GGGGCGGGCG CCTGCGTCGG TCTCCGCGAA GCGGGAAAGC GCGGCGGCCG CCGGGATTCG 2761 GGCGCCGCGG CAGCTGCTCC GGCTGCCGGC CGGCGGCCCC GCGCTCGCCC GCCCCGCTTC 2821 CGCCCGCTGT CCTGCTGCAC GAACCCTTCC AACTCTCCTT TCCTCCCCCA CCCTTGAGTT 2881 ACCCCTCTGT CTTTCCTGCT GTTGCGCGGG TGCTCCCACA GCGGAGCGGA GATTACAGAG 2941 CCGCCGGGAT GCCCGCTGGA TCCATGGaag acgccaaaaa cataaagaaa ggcccggcgc 3001 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat 3061 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt 3121 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga 3181 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt 3241 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg 3301 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt 3361 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg 3421 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac 3481 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg 3541 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata 3601 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc 3661 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac 3721 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt 3781 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct 3841 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg 3901 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc 3961 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac 4021 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg 4081 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga 4141 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg 4201 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct 4261 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg 4321 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc 4381 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga 4441 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc 4501 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa 4561 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaatTCT 4621 AGAgcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac 4681 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 4741 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 4801 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa 4861 aatcgataag tttaaacaga tccaggtggc acttttcggg gaaatgtgcg cggaacccct 4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 6661 ttcctggcct tttgctggcc ttttgctcac atggctcgac //