LOCUS pEGFP-CAT-EMCV_137 6079 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 8..582 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 583..589 /label=immediate early CMV promoter(1) /ApEinfo_label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 3791..3926 /label=SV40 origin of replication /ApEinfo_fwdcolor=#eb3c34 /ApEinfo_revcolor=#eb3c34 misc_feature 2898..2903 /label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 5018..5023 /label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 1370..2006 /label=PCR-product-cleaved-HindIII-BamHI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3814..3834 /label=21-bp repeat /ApEinfo_fwdcolor=#ffab80 /ApEinfo_revcolor=#ffab80 misc_feature 2766..2792 /label=rev-primer /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 2936..2948 /label=mRNA ends somewhere here /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3843..3843 /label=major transcription start /ApEinfo_fwdcolor=#ff21ff /ApEinfo_revcolor=#ff21ff misc_feature 1396..1982 /label=EMCV_IRES /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=#0080c0 misc_feature 5354..5997 /label=pUC plasmid replication origin /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 59..465 /label=enhancer /ApEinfo_fwdcolor=#ff3b69 /ApEinfo_revcolor=#ff3b69 misc_feature 2995..3450 /label=f1 single strand DNA origin /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3881..3881 /label=major transcription start(1) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff36ff /ApEinfo_revcolor=#ff36ff misc_feature 3624..3695 /label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff2649 /ApEinfo_revcolor=#ff2649 misc_feature 554..560 /label=TATA box /ApEinfo_fwdcolor=#ff4899 /ApEinfo_revcolor=#ff4899 misc_feature 3512..3517 /label=-35 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3887..3887 /label=major transcription start(2) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff33ff /ApEinfo_revcolor=#ff33ff misc_feature 3696..3767 /label=enhancer (72-bp tandem repeat)(1) /ApEinfo_label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff5059 /ApEinfo_revcolor=#ff5059 CDS 3975..4769 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=DarkOrange1 /ApEinfo_revcolor=DarkOrange1 misc_feature 3535..3540 /label=-10 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3892..3892 /label=major transcription start(3) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff41ff /ApEinfo_revcolor=#ff41ff misc_feature 3771..3791 /label=21-bp repeat(1) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff7890 /ApEinfo_revcolor=#ff7890 misc_feature 3547..3547 /label=transcription start (+1) of Kan/Neo resistance /ApEinfo_fwdcolor=#ff4e54 /ApEinfo_revcolor=#ff4e54 misc_feature 5005..5010 /label=HSV polyA signal(1) /ApEinfo_label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3792..3812 /label=21-bp repeat(2) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff96a6 /ApEinfo_revcolor=#ff96a6 misc_feature 2025..2744 /label=EGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 2927..2932 /label=SV40 early polyA signal(1) /ApEinfo_label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 583..583 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff19ff /ApEinfo_revcolor=#ff19ff misc_feature 652..1311 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG 721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG 901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA 961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT 1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG 1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA 1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA 1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTATCTAG 1381 GGCGGCCAAT TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG 1441 GAATAAGGCC GGTGTGCGTT TGTCTATATG TGATTTTCCA CCATATTGCC GTCTTTTGGC 1501 AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC 1561 CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA 1621 GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT 1681 GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA 1741 CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA 1801 AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT 1861 CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC 1921 CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAAGC TTGCCACAAC 1981 CCGGGATAAT TCCTGCAGCC AATATGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG 2041 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 2101 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 2161 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA 2221 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 2281 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 2341 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 2401 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 2461 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT 2521 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA 2581 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT 2641 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA 2701 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC CGCGACTCTA 2761 GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA 2821 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC 2881 AGCTTATAAT GGTTACAaat aaagcaatag catcacaaat ttcacaaata aagcattttt 2941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 3001 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 3061 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 3121 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 3181 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 3241 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 3301 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 3361 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 3421 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 3481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 3541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 3601 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 3661 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 3721 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 3781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 3841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 3901 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 3961 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4021 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4081 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4141 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4201 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4261 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 4321 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 4381 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4441 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4501 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 4561 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4621 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4681 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4741 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4801 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4861 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4921 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 4981 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 5041 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 5101 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 5161 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 5221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 5281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 5341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 5401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 5461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 5521 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 5581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 5641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 5701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 5761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 5821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 5881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 5941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 6001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 6061 ccgtattacc gccatgcat //