LOCUS pEGFP-CAT-NDST3 5698 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 8..589 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 583..583 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff19ff /ApEinfo_revcolor=#ff19ff misc_feature 1644..2363 /label=EGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 CDS 3594..4388 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=DarkOrange1 /ApEinfo_revcolor=DarkOrange1 misc_feature 2614..3069 /label=f1 single strand DNA origin /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3410..3545 /label=SV40 origin of replication /ApEinfo_fwdcolor=#eb3c34 /ApEinfo_revcolor=#eb3c34 misc_feature 3511..3511 /label=major transcription start /ApEinfo_fwdcolor=#ff41ff /ApEinfo_revcolor=#ff41ff misc_feature 3243..3314 /label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff2649 /ApEinfo_revcolor=#ff2649 misc_feature 3433..3453 /label=21-bp repeat /ApEinfo_fwdcolor=#ffab80 /ApEinfo_revcolor=#ffab80 misc_feature 1375..1625 /label=NDST3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 2555..2567 /label=mRNA ends somewhere here /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3166..3166 /label=transcription start (+1) of Kan/Neo resistance /ApEinfo_fwdcolor=#ff4e54 /ApEinfo_revcolor=#ff4e54 misc_feature 3506..3506 /label=major transcription start(1) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff33ff /ApEinfo_revcolor=#ff33ff misc_feature 4973..5616 /label=pUC plasmid replication origin /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 3411..3431 /label=21-bp repeat(1) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff96a6 /ApEinfo_revcolor=#ff96a6 misc_feature 2517..2522 /label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3131..3136 /label=-35 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3462..3462 /label=major transcription start(2) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff21ff /ApEinfo_revcolor=#ff21ff misc_feature 4624..4629 /label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3315..3386 /label=enhancer (72-bp tandem repeat)(1) /ApEinfo_label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff5059 /ApEinfo_revcolor=#ff5059 misc_feature 2546..2551 /label=SV40 early polyA signal(1) /ApEinfo_label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3154..3159 /label=-10 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3500..3500 /label=major transcription start(3) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff36ff /ApEinfo_revcolor=#ff36ff misc_feature 4637..4642 /label=HSV polyA signal(1) /ApEinfo_label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3390..3410 /label=21-bp repeat(2) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff7890 /ApEinfo_revcolor=#ff7890 misc_feature 652..1311 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG 721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG 901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA 961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT 1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG 1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA 1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA 1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTCTGGGG 1381 GAGGCGGCTG CAGCCGTGGC TCTGCAGCGA GAGCGCAGAG AAGCAGAGGA CCAAGAGGCA 1441 GAATGCCGGC GCTCCACCGC CCGAGACTGT GTTTCCTGTG AGTCCAGATG TGTGATCCAG 1501 ATATGCTGCA ATGGTGACAT AAACTATTGA CTGAGACTAG GAGCGTGCCT GAAACTCCAT 1561 CCCTCCTCCT ATTATTTTTT TAATGGTGCT TCTACTGGAA TAATCAACGA AGTGCACCCA 1621 ACATGGATCC ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG 1681 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG 1741 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 1801 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA 1861 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT 1921 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG 1981 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG 2041 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA 2101 TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG 2161 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC 2221 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA 2281 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG 2341 GCATGGACGA GCTGTACAAG TAAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC 2401 ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA 2461 TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAaata 2521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 2581 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa 2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 3061 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 3181 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt 3241 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 3301 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 3361 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 3421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 3481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 3541 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg 3601 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 3661 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 3721 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 3781 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 3841 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 3901 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 3961 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 4021 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 4081 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 4141 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 4201 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 4261 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 4321 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 4381 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 4441 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 4501 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 4561 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 4621 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt 4681 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc 4741 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 4801 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat 4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 5641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat //