LOCUS pEMCV 7330 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 794..812 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 811..811 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 497..796 /label=left half of vaccinia thimidine kinase /ApEinfo_fwdcolor=#003bff /ApEinfo_revcolor=#003bff misc_feature 1..63 /label=N-terminus of vaccinia putative membrane protein /ApEinfo_fwdcolor=#00ff3c /ApEinfo_revcolor=#00ff3c misc_feature 20..481 /label=vaccinia virion protein VACWR093 /ApEinfo_fwdcolor=#00ff5e /ApEinfo_revcolor=#00ff5e misc_feature 6916..6916 /label=oriC_from_pUC19 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 867..1418 /label=EMCV_R IRES since poly(C) to -7 (gataatATG missing) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 5297..6157 /label=beta lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 3426..3465 /label=right part of MCS since BamHI /ApEinfo_fwdcolor=#db6bff /ApEinfo_revcolor=#db6bff misc_feature 2652..3455 /label=eGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 1425..2084 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 2099..2645 /label=EMCV_R IRES since the poly(C) to -7 (gataaATG missing) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3472..3595 /label=T7 transcription terminator Tphi /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3576..3576 /label=T7 transcription terminator Tphi site /ApEinfo_fwdcolor=#ff10ff /ApEinfo_revcolor=#ff10ff ORIGIN 1 AAGCTTTTGC GATCAATAAA TGGATCACAA CCAGTATCTC TTAACGATGT TCTTCGCAGA 61 TGATGATTCA TTTTTTAAGT ATTTGGCTAG TCAAGATGAT GAATCTTCAT TATCTGATAT 121 ATTGCAAATC ACTCAATATC TAGACTTTCT GTTATTATTA TTGATCCAAT CAAAAAATAA 181 ATTAGAAGCC GTGGGTCATT GTTATGAATC TCTTTCAGAG GAATACAGAC AATTGACAAA 241 ATTCACAGAC TTTCAAGATT TTAAAAAACT GTTTAACAAG GTCCCTATTG TTACAGATGG 301 AAGGGTCAAA CTTAATAAAG GATATTTGTT CGACTTTGTG ATTAGTTTGA TGCGATTCAA 361 AAAAGAATCC TCTCTAGCTA CCACCGCAAT AGATCCTGTT AGATACATAG ATCCTCGTCG 421 CAATATCGCA TTTTCTAACG TGATGGATAT ATTAAAGTCG AATAAAGTGA ACAATAATTA 481 ATTCTTTATT GTCATCATGA ACGGCGGACA TATTCAGTTG ATAATCGGCC CCATGTTTTC 541 AGGTAAAAGT ACAGAATTAA TTAGACGAGT TAGACGTTAT CAAATAGCTC AATATAAATG 601 CGTGACTATA AAATATTCTA ACGATAATAG ATACGGAACG GGACTATGGA CGCATGATAA 661 GAATAATTTT GAAGCATTGG AAGCAACTAA ACTATGTGAT GTCTTGGAAT CAATTACAGA 721 TTTCTCCGTG ATAGGTATCG ATGAAGGACA GTTCTTTCCA GACATTGTTG AATTGATCTC 781 GATCCCGCGA AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGCGGG 841 ATCAATTCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT 901 AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG 961 TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC 1021 TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT 1081 CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG 1141 ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC 1201 CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG 1261 TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG 1321 GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC 1381 GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATAA TACCATGGAG AAAAAAATCA 1441 CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC 1501 AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA 1561 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC 1621 TGATGAATGC TCATCCGGAA CTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG 1681 ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT 1741 GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT 1801 GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT 1861 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT 1921 TCTTCGCCCC CGTTTTCACG ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC 1981 CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA 2041 ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGC GCTACCGGTA 2101 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT 2161 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA 2221 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG 2281 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT 2341 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT 2401 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG 2461 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG 2521 TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT 2581 CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC 2641 ACGATAATAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGCCC ATCCTGGTCG 2701 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG 2761 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT 2821 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC 2881 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA 2941 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG 3001 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC 3061 TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC 3121 AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC 3181 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG 3241 ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC 3301 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT 3361 ACAAGTCCGG ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG 3421 GCCCGGGATC CCTGCAGCTC GAGAGGCCTA ATTAATTAAG TCGACGATCC GGCTGCTAAC 3481 AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC 3541 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA 3601 TCGAGATCAA TTCTGTGAGC GTATGGCAAA CGAAGGAAAA ATAGTTATAG TAGCCGCACT 3661 CGATGGGACA TTTCAACGTA AACCGTTTAA TAATATTTTG AATCTTATTC CATTATCTGA 3721 AATGGTGGTA AAACTAACTG CTGTGTGTAT GAAATGCTTT AAGGAGGCTT CCTTTTCTAA 3781 ACGATTGGGT GAGGAAACCG AGATAGAAAT AATAGGAGGT AATGATATGT ATCAATCGGT 3841 GTGTAGAAAG TGTTACATCG ACTCATAATA TTATATTTTT TATCTAAAAA ACTAAAAATA 3901 AACATTGCTT AAATTTTAAT ATAATACTTA AAAATGGATG TTGTGTCGTT AGATAAACCG 3961 TTTATGTATT TTGAGGAAAT TGATAATGAG TTAGATTACG AACCAGAAAG TGCAAATGAG 4021 GTCGCAAAAA AACTGCCGTA TCAAGGACAG TTAAAACTAT TACTAGGAGA ATTATTTTTT 4081 CTTAGTAAGT TACAGCGACA CGGTATATTA GATGGTGCCA CCGTAGTGTA TATAGGATCT 4141 GCTCCCGGTA CACATATACG TTATTTGAGA GATCATTTCT ATAATTTAGG AGTGATCATC 4201 AAATGGATGC TAATTGACGG CCGCCATCAT GATCCTATTT TAAATGGATT GCGTGATGTG 4261 ACTCTAGTGA CTCGGTTCGT TGATGAGGAA TATCTACGAT CCATCAAAAA ACAACTGCAT 4321 CCTTCTAAGA TTATTTTAAT TTCTGATGTG AGATCCAAAC GAGGAGGAAA TGAACCTAGT 4381 ACGGCGGATT TACTAAGTAA TTACGCTCTA CAAAATGTCA TGATTAGTAT TTTAAACCCC 4441 GTGGCGTCTA GTCTTAAATG GAGATGCCCG TTTCCAGATC AATGGATCAA GGACTTTTAT 4501 ATCCCACACG GTAATAAAAT GTTACAACCT TTTGCTCCTT CATATTCAGG GCCGTCGTTT 4561 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 4621 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 4681 TGCGCAGCCT GAATGGCGAA TGGCGCGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 4741 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 4801 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 4861 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 4921 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 4981 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 5041 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 5101 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA 5161 TTTCCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 5221 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 5281 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 5341 GGCATTTTGC CTTCCTCTTT TTGCTCACCC AGAAAGCGTG GTGAAAGTAA AAGATGCTGA 5401 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 5461 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 5521 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 5581 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 5641 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 5701 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 5761 TCATCTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 5821 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 5881 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 5941 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 6001 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 6061 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 6121 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 6181 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 6241 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 6301 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 6361 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 6421 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 6481 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 6541 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 6601 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 6661 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 6721 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 6781 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 6841 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 6901 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 6961 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 7021 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 7081 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 7141 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 7201 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 7261 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA 7321 TGATTACGCC //