LOCUS pEB_351-920 7361 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 794..812 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 811..811 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 3457..3496 /label=right part of MCS since BamHI /ApEinfo_fwdcolor=#db6bff /ApEinfo_revcolor=#db6bff misc_feature 2683..3486 /label=eGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 497..796 /label=left half of vaccinia thimidine kinase /ApEinfo_fwdcolor=#003bff /ApEinfo_revcolor=#003bff misc_feature 1..63 /label=N-terminus of vaccinia putative membrane protein /ApEinfo_fwdcolor=#00ff3c /ApEinfo_revcolor=#00ff3c misc_feature 20..481 /label=vaccinia virion protein VACWR093 /ApEinfo_fwdcolor=#00ff5e /ApEinfo_revcolor=#00ff5e misc_feature 3503..3626 /label=T7 transcription terminator Tphi /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 6947..6947 /label=oriC_from_pUC19 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3607..3607 /label=T7 transcription terminator Tphi site /ApEinfo_fwdcolor=#ff10ff /ApEinfo_revcolor=#ff10ff misc_feature 867..1418 /label=EMCV_R IRES since poly(C) to -7 (gataatATG missing) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 5328..6188 /label=beta lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1425..2084 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 2100..2669 /label=ERBV IRES 351-920 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan ORIGIN 1 AAGCTTTTGC GATCAATAAA TGGATCACAA CCAGTATCTC TTAACGATGT TCTTCGCAGA 61 TGATGATTCA TTTTTTAAGT ATTTGGCTAG TCAAGATGAT GAATCTTCAT TATCTGATAT 121 ATTGCAAATC ACTCAATATC TAGACTTTCT GTTATTATTA TTGATCCAAT CAAAAAATAA 181 ATTAGAAGCC GTGGGTCATT GTTATGAATC TCTTTCAGAG GAATACAGAC AATTGACAAA 241 ATTCACAGAC TTTCAAGATT TTAAAAAACT GTTTAACAAG GTCCCTATTG TTACAGATGG 301 AAGGGTCAAA CTTAATAAAG GATATTTGTT CGACTTTGTG ATTAGTTTGA TGCGATTCAA 361 AAAAGAATCC TCTCTAGCTA CCACCGCAAT AGATCCTGTT AGATACATAG ATCCTCGTCG 421 CAATATCGCA TTTTCTAACG TGATGGATAT ATTAAAGTCG AATAAAGTGA ACAATAATTA 481 ATTCTTTATT GTCATCATGA ACGGCGGACA TATTCAGTTG ATAATCGGCC CCATGTTTTC 541 AGGTAAAAGT ACAGAATTAA TTAGACGAGT TAGACGTTAT CAAATAGCTC AATATAAATG 601 CGTGACTATA AAATATTCTA ACGATAATAG ATACGGAACG GGACTATGGA CGCATGATAA 661 GAATAATTTT GAAGCATTGG AAGCAACTAA ACTATGTGAT GTCTTGGAAT CAATTACAGA 721 TTTCTCCGTG ATAGGTATCG ATGAAGGACA GTTCTTTCCA GACATTGTTG AATTGATCTC 781 GATCCCGCGA AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGCGGG 841 ATCAATTCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT 901 AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG 961 TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC 1021 TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT 1081 CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG 1141 ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC 1201 CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG 1261 TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG 1321 GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC 1381 GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATAA TACCATGGAG AAAAAAATCA 1441 CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC 1501 AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA 1561 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC 1621 TGATGAATGC TCATCCGGAA CTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG 1681 ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT 1741 GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT 1801 GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT 1861 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT 1921 TCTTCGCCCC CGTTTTCACG ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC 1981 CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA 2041 ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGC GCTACCGGTC 2101 GTTCCACTTT AGCAGGGGTC TTCTTTCTAT CCCCCTGGCG GCATTGGAAC TAGCCGTCGC 2161 GTCTTAACGC GCAGCCCTGA AGGCCCCACA CCTTGTGGAT CTTGCCGTGG GTATGTTTCT 2221 GGCATGTGTT TCTCAAGCCT GCAACCGAAG CCGAACAGCC ACATGAACAG TTTGAGCGTG 2281 GTAGCGCTGT GTGAGTTGGC GGTGGATCCC CCTCGTGGTA ACACGAGCCC CCGTGGCCAA 2341 AAGCCCAGTG TTTACAGCAC CTCTCACATC CAGGACGACC CCATCCTGGC GCTCACTCTT 2401 AGTAGTATGG CTTAGTACGC ATTAGGTGGT AAGCCGAGCT CTCCCTCGGC CTTGTTCTGA 2461 ATGCACACAT GTCTAGGGGC TAAGGATGTC CTACAGGTAC CCGCACGTAA CCTTCAGAGA 2521 GTGCGGATCT GAGTAGGAGA CCGTGGTGCA CTGCTTTACA GATGCAGCCC CGGTTTAAAA 2581 AGCGTCTATG CCCCTACAGG GTAGCGGTGG GCCGCGCCCT TTCCTTTTAA AACTACTTGT 2641 TCTATGGTGA CAATGGCAGG AAACATGATA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG 2701 GAGCTGTTCA CCGGGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 2761 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 2821 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 2881 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 2941 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 3001 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 3061 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 3121 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 3181 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 3241 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 3301 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 3361 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TCGAGCTCAA 3421 GCTTCGAATT CTGCAGTCGA CGGTACCGCG GGCCCGGGAT CCCTGCAGCT CGAGAGGCCT 3481 AATTAATTAA GTCGACGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC 3541 TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG 3601 TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATCGAGATCA ATTCTGTGAG CGTATGGCAA 3661 ACGAAGGAAA AATAGTTATA GTAGCCGCAC TCGATGGGAC ATTTCAACGT AAACCGTTTA 3721 ATAATATTTT GAATCTTATT CCATTATCTG AAATGGTGGT AAAACTAACT GCTGTGTGTA 3781 TGAAATGCTT TAAGGAGGCT TCCTTTTCTA AACGATTGGG TGAGGAAACC GAGATAGAAA 3841 TAATAGGAGG TAATGATATG TATCAATCGG TGTGTAGAAA GTGTTACATC GACTCATAAT 3901 ATTATATTTT TTATCTAAAA AACTAAAAAT AAACATTGCT TAAATTTTAA TATAATACTT 3961 AAAAATGGAT GTTGTGTCGT TAGATAAACC GTTTATGTAT TTTGAGGAAA TTGATAATGA 4021 GTTAGATTAC GAACCAGAAA GTGCAAATGA GGTCGCAAAA AAACTGCCGT ATCAAGGACA 4081 GTTAAAACTA TTACTAGGAG AATTATTTTT TCTTAGTAAG TTACAGCGAC ACGGTATATT 4141 AGATGGTGCC ACCGTAGTGT ATATAGGATC TGCTCCCGGT ACACATATAC GTTATTTGAG 4201 AGATCATTTC TATAATTTAG GAGTGATCAT CAAATGGATG CTAATTGACG GCCGCCATCA 4261 TGATCCTATT TTAAATGGAT TGCGTGATGT GACTCTAGTG ACTCGGTTCG TTGATGAGGA 4321 ATATCTACGA TCCATCAAAA AACAACTGCA TCCTTCTAAG ATTATTTTAA TTTCTGATGT 4381 GAGATCCAAA CGAGGAGGAA ATGAACCTAG TACGGCGGAT TTACTAAGTA ATTACGCTCT 4441 ACAAAATGTC ATGATTAGTA TTTTAAACCC CGTGGCGTCT AGTCTTAAAT GGAGATGCCC 4501 GTTTCCAGAT CAATGGATCA AGGACTTTTA TATCCCACAC GGTAATAAAA TGTTACAACC 4561 TTTTGCTCCT TCATATTCAG GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 4621 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 4681 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCGAC 4741 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 4801 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 4861 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4921 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4981 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 5041 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 5101 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 5161 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCCCAGG TGGCACTTTT CGGGGAAATG 5221 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 5281 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 5341 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTCTT TTTGCTCACC 5401 CAGAAAGCGT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 5461 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 5521 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 5581 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 5641 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 5701 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 5761 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATCTAAC TCGCCTTGAT CGTTGGGAAC 5821 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 5881 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 5941 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 6001 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 6061 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 6121 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 6181 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 6241 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 6301 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 6361 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 6421 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 6481 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 6541 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 6601 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 6661 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 6721 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 6781 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 6841 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 6901 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 6961 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 7021 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 7081 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 7141 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 7201 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 7261 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 7321 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC C //