LOCUS pRF_138_to__86 7208 bp ds-DNA circular 15-JUL-2008 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 34..347 /vntifkey="29" /label=SV40\promoter\and\enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 721..1656 /vntifkey="4" /label=RLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 4157..4393 /label=Enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 2232..3884 /vntifkey="4" /label=FLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 348..451 /label=SV40 Promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 348..397 /label=SV40 transcription start sites region /ApEinfo_fwdcolor=#ffb1be /ApEinfo_revcolor=#ffb1be rep_origin 4708..4708 /vntifkey="33" /label=ColE1-derived\plasmid\replication\origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 516..648 /label=chimeric intron /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan CDS complement(5470..6330) /vntifkey="4" /label=beta-lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 693..711 /label=T7 promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 392..392 /label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff rep_origin 6462..6917 /vntifkey="33" /label=F1\Origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 710..710 /label=T7 transcription start (+1) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 397..397 /label=SV40 transcription start (+1)(1) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff polyA_signal 7048..7201 /vntifkey="25" /label=Synthetic\(upstream)\polyA\signal /ApEinfo_fwdcolor=#ff00cb /ApEinfo_revcolor=#ff00cb misc_feature 348..348 /label=SV40 transcription start (+1)(2) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 1672..2170 /vntifkey="21" /label=deltaEMCV /ApEinfo_fwdcolor=#d0ff00 /ApEinfo_revcolor=#d0ff00 misc_feature 386..386 /label=SV40 transcription start (+1)(3) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#fe00ff /ApEinfo_revcolor=#fe00ff misc_feature 3916..4137 /label=SV40 Late poly(A) signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 2171..2176 /label=EcoRI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 2230..2235 /label=NcoI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 2177..2229 /label=LINE1 -138 to -86 from ORF2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccga tctgcgcagc accatggcct gaaataacct 61 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt 121 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 181 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 241 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 301 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgaCTA ATTTTTTTTA 361 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 421 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC Ttgattcttc tgacacaaca gtctcgaact 481 taagctgcag aagttggtcg tgaggcactg ggcagGTAAG TATCAAGGTT ACAAGACAGG 541 TTTAAGGAGA CCAATAGAAA CTGGGCTTGT CGAGACAGAG AAGACTCTTG CGTTTCTGAT 601 AGGCACCTAT TGGTCTTACT GACATCCACT TTGCCTTTCT CTCCACAGgt gtccactccc 661 agttcaatta cagctcttaa ggctagagta ctTAATACGA CTCACTATAG Gctagccacc 721 atgacttcga aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg 781 tgggccagat gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa 841 aaacatgcag aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg 901 cgacatgttg tgccacatat tgagccagta gcgcggtgta ttataccaga ccttattggt 961 atgggcaaat caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat 1021 cttactgcat ggtttgaact tcttaattta ccaaagaaga tcatttttgt cggccatgat 1081 tggggtgctt gtttggcatt tcattatagc tatgagcatc aagataagat caaagcaata 1141 gttcacgctg aaagtgtagt agatgtgatt gaatcatggg atgaatggcc tgatattgaa 1201 gaagatattg cgttgatcaa atctgaagaa ggagaaaaaa tggttttgga gaataacttc 1261 ttcgtggaaa ccatgttgcc atcaaaaatc atgagaaagt tagaaccaga agaatttgca 1321 gcatatcttg aaccattcaa agagaaaggt gaagttcgtc gtccaacatt atcatggcct 1381 cgtgaaatcc cgttagtaaa aggtggtaaa cctgacgttg tacaaattgt taggaattat 1441 aatgcttatc tacgtgcaag tgatgattta ccaaaaatgt ttattgaatc ggacccagga 1501 ttcttttcca atgctattgt tgaaggtgcc aagaagtttc ctaatactga atttgtcaaa 1561 gtaaaaggtc ttcatttttc gcaagaagat gcacctgatg aaatgggaaa atatatcaaa 1621 tcgttcgttg agcgagttct caaaaatgaa caataattct aggggctcga gcgcccctct 1681 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 1741 gtctatatgt tattttccac catattgccg tcttttggca acgtgagggc ccggaaacct 1801 ggccctgtct tcttgacgag caatcctaga gcttcttgaa gacaaacaac gtctgtagcg 1861 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 1921 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 1981 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 2041 cagaaggtac ccccttgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 2101 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt 2161 tgaaaaacac gaattcCGCA TTATCTCTCC ACGAATCCAG CCCTTCAAAG GATAATAACA 2221 GAAAAAAACc catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc 2281 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc 2341 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact 2401 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca 2461 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat 2521 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca 2581 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt 2641 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg 2701 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta 2761 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga 2821 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg 2881 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga 2941 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga 3001 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc 3061 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca 3121 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg 3181 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca 3241 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg 3301 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg 3361 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga 3421 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat 3481 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc 3541 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca 3601 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg 3661 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag 3721 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg 3781 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag 3841 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg 3901 gccggccgct tcgagCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 3961 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 4021 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 4081 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAaaa 4141 tcgataagga tctgaaCGAT GGAGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 4201 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 4261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 4321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 4381 CACATTCCAC AGCggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc 4441 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg 4501 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg 4561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 4681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 4861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 5161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 5281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 5641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 5701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 5761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 5821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 5881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc 6481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 6541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 6601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 6661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 6721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 6781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 6841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 6901 attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 6961 ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa 7021 ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt 7081 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa 7141 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc 7201 tatcgata //