LOCUS _620_1222_CAT_Ires 7646 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 620-1222/CAT standard; circular DNA; ; 7646 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493820090| CC VNTDBDATE|493820090| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 2418..3264 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 6732..7592 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal 3300..3531 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink rep_origin complement(5955..6637) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 polyA_signal complement(3073..3264) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 4072..4397 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4433..5227 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(3281..3298) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5567..5587) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(5593..5615) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 3611..3917 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 5402..5532 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 1060..1662 /label=Hiap2_620-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1670..2328 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgaga attacttttg tttattggaa 1081 taaaatgaga ttttttgggt tgtcatgtta aagtgcttat agggaaagaa gcctgcatat 1141 aattttttac cttgtggcat aatcagtaat tggtctgtta ttcaggcttc atagcttgta 1201 accaaatata aataaaaggc ataatttagg tattctatag ttgcttagaa ttttgttaat 1261 ataaatctct gtgaaaaatc aaggagtttt aatattttca gaagtgcatc cacctttcag 1321 ggctttaagt tagtattact caagattatg aacaaatagc acttaggtta cctgaaagag 1381 ttactacaac cccaaagagt tgtgttctaa gtagtatctt ggtaattcag agagatactc 1441 atcctacctg aatataaact gagataaatc cagtaaagaa agtgtagtaa attctacata 1501 agagtctatc attgatttct ttttgtggta aaaatcttag ttcatgtgaa gaaatttcat 1561 gtgaatgttt tagctatcaa acagtactgt cacctactca tgcacaaaac tgcctcccaa 1621 agacttttcc caggtccctc gtatcaaaac attaagagta tactcgagat ggagaaaaaa 1681 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca 1741 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt 1801 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc 1861 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata 1921 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg 1981 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg 2041 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc 2101 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac 2161 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg 2221 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg 2281 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc 2341 agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga 2401 atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg 2461 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa 2521 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat 2581 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg 2641 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag 2701 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct 2761 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca 2821 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt 2881 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat 2941 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa 3001 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt 3061 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 3121 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 3181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 3241 catcaatgta tcttatcatg tctgcctaga gggccctatt ctatagtgtc acctaaatgc 3301 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 3361 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 3421 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 3481 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 3541 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 3601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 3661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 3721 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt 3781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 3841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 3901 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 3961 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4021 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg 4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4321 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4381 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4441 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4561 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4621 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 4981 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5041 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5161 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5221 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5521 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 5581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 5641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 5701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 5761 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 5821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 5881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 5941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 6181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 6421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 6601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 6661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 6721 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 6781 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 6841 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 6901 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 6961 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 7021 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 7081 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 7141 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7201 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 7261 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 7321 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 7381 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 7441 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 7501 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 7561 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 7621 atttatcagg gttattgtct catgag //