LOCUS _870_1222_CAT_Ires 7396 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 870-1222/CAT standard; circular DNA; ; 7396 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493820168| CC VNTDBDATE|493820168| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 2168..3014 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal 3050..3281 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink promoter 3822..4147 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 6482..7342 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(5705..6387) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 4183..4977 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal complement(2823..3014) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 3361..3667 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 primer_bind complement(3031..3048) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5317..5337) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(5343..5365) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5152..5282 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 1060..1412 /label=Hiap2_870-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1419..2078 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagc cacctttcag ggctttaagt 1081 tagtattact caagattatg aacaaatagc acttaggtta cctgaaagag ttactacaac 1141 cccaaagagt tgtgttctaa gtagtatctt ggtaattcag agagatactc atcctacctg 1201 aatataaact gagataaatc cagtaaagaa agtgtagtaa attctacata agagtctatc 1261 attgatttct ttttgtggta aaaatcttag ttcatgtgaa gaaatttcat gtgaatgttt 1321 tagctatcaa acagtactgt cacctactca tgcacaaaac tgcctcccaa agacttttcc 1381 caggtccctc gtatcaaaac attaagagta tactcgagat ggagaaaaaa atcactggat 1441 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 1501 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 1561 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 1621 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg 1681 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 1741 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 1801 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 1861 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 1921 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 1981 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat 2041 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt 2101 gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa 2161 ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac 2221 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac 2281 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc 2341 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg 2401 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag 2461 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta 2521 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca 2581 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata 2641 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc 2701 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt 2761 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct 2821 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 2881 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3001 tcttatcatg tctgcctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc 3061 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 3121 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 3181 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 3241 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 3301 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 3361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3481 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc 3541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt 3721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 3781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg 3841 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 4021 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 4081 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 4141 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 4201 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 4261 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 4321 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 4381 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 4441 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 4501 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 4561 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 4621 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 4681 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 4741 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 4801 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 4861 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 4921 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 4981 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 5041 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 5101 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 5161 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5281 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 5341 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5401 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5461 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5881 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 5941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6121 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 6181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 6301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 6361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 6421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 6481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 6541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 6601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 6661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 6841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 6901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 6961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 7021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 7081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 7141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 7201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 7261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 7321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 7381 gttattgtct catgag //