LOCUS pGEM3_IRES 3328 bp ds-DNA circular 12-FEB-2008 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers source complement(1..2810) /organism="Cloning vector pGEM-3" /mol_type="other DNA" /db_xref="taxon:90108" /label=source:Cloning vector pGEM-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(3328..3328) /note="SP6 transcription initiation site" /label=SP6 transcription initiation site /ApEinfo_fwdcolor=#00ffde /ApEinfo_revcolor=#00ffde misc_feature complement(2805..2810) /note="multiple cloning sites" /label=multiple cloning sites /ApEinfo_fwdcolor=#00ffde /ApEinfo_revcolor=#00ffde promoter complement(2779..2801) /note="T7 promoter" /label=T7 promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature complement(2796..2796) /note="T7 transcription initiation site" /label=T7 transcription initiation site /ApEinfo_fwdcolor=#00ffde /ApEinfo_revcolor=#00ffde misc_feature complement(2710..2768) /note="lac operon sequence" /label=lac operon sequence /ApEinfo_fwdcolor=#00ffde /ApEinfo_revcolor=#00ffde gene 908..1768 /gene="bla" /label=bla /ApEinfo_fwdcolor=#00ff6e /ApEinfo_revcolor=#00ff6e CDS 908..1768 /gene="bla" /codon_start=1 /transl_table=11 /product="Beta-lactamase" /protein_id="CAA46394.1" /db_xref="GI:58200" /db_xref="InterPro:IPR000871" /db_xref="InterPro:IPR001466" /db_xref="InterPro:IPR012338" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /label=Beta-lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 promoter complement(1..18) /note="SP6 promoter" /label=SP6 promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3272..3277 /label=XhoI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 source complement(3272..3328) /organism="Cloning vector pGEM-3" /mol_type="other DNA" /db_xref="taxon:90108" /label=source:Cloning vector pGEM-3(1) /ApEinfo_label=source:Cloning vector pGEM-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(3272..3322) /note="multiple cloning sites" /label=multiple cloning sites(1) /ApEinfo_label=multiple cloning sites /ApEinfo_fwdcolor=#00ffde /ApEinfo_revcolor=#00ffde misc_feature 2811..3271 /label=FMDV IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan ORIGIN 1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc 61 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc 121 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt 181 ctggtaacgc cgttccgcac cccggaaatg gtcagcgaac caatcagcag ggtcatcgct 241 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt 301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 421 gccatctcct tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 481 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg atatggtgca ctctcagtac 541 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 601 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 661 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 721 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 1021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 1081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 1141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 1201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 1261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 1321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 1381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 1441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 1501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 1561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 1621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 1681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 1741 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 1801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 1861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 1921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 1981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 2041 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 2101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 2161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 2221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 2281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 2341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 2401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 2521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 2581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 2641 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 2701 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 2761 cagctggctt atcgaaatta atacgactca ctatagggag accggaattc AGCAGGTTTC 2821 CCCAACTGAC ACAAAACGTG CAACTTGAAA CTCCGCCTGG TCTTTCCAGG TCTAGAGGGG 2881 TAACACTTTG TACTGTGTTT GGCTCCACGC TCGATCCACT GGCGAGTGTT AGTAACAGCA 2941 CTGTTGCTTC GTAGCGGAGC ATGACGGCCG TGGGAACTCC TCCTTGGTAA CAAGGACCCA 3001 CGGGGCCAAA AGCCACGCCC ACACGGGCCC GTCATGTGTG CAACCCCAGC ACGGCGACTT 3061 TACTGCGAAA CCCACTTTAA AGTGACATTG AAACTGGTAC CCACACACTG GTGACAGGCT 3121 AAGGATGCCC TTCAGGTACC CCGAGGTAAC ACGCGACACT CGGGATCTGA GAAGGGGACT 3181 GGGGCTTCTA TAAAAGCGCT CGGTTTAAAA AGCTTCTACG CCTGAATAGG TGACCGGAGG 3241 TCGGCACCTT TCCTTTACAA TTAATGACCC Tgagctcggt acccggggat cctctagagt 3301 cgacctgcag gcatgcaagc ttgtattc //