LOCUS pRF_D 7355 bp ds-DNA circular 14-JUL-2008 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 34..347 /vntifkey="29" /label=SV40\promoter\and\enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 721..1656 /vntifkey="4" /label=RLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 4304..4540 /label=Enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 2379..4031 /vntifkey="4" /label=FLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 348..451 /label=SV40 Promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 348..397 /label=SV40 transcription start sites region /ApEinfo_fwdcolor=#ffb1be /ApEinfo_revcolor=#ffb1be rep_origin 4855..4855 /vntifkey="33" /label=ColE1-derived\plasmid\replication\origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 516..648 /label=chimeric intron /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan CDS complement(5617..6477) /vntifkey="4" /label=beta-lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 693..711 /label=T7 promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 392..392 /label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff rep_origin 6609..7064 /vntifkey="33" /label=F1\Origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 710..710 /label=T7 transcription start (+1) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 397..397 /label=SV40 transcription start (+1)(1) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff polyA_signal 7195..7348 /vntifkey="25" /label=Synthetic\(upstream)\polyA\signal /ApEinfo_fwdcolor=#ff00cb /ApEinfo_revcolor=#ff00cb misc_feature 2176..2376 /vntifkey="21" /label=LINE-1 ORF2 -201nt/-1 /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 348..348 /label=SV40 transcription start (+1)(2) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 1672..2170 /vntifkey="21" /label=deltaEMCV /ApEinfo_fwdcolor=#d0ff00 /ApEinfo_revcolor=#d0ff00 misc_feature 386..386 /label=SV40 transcription start (+1)(3) /ApEinfo_label=SV40 transcription start (+1) /ApEinfo_fwdcolor=#fe00ff /ApEinfo_revcolor=#fe00ff misc_feature 4063..4284 /label=SV40 Late poly(A) signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 2171..2176 /label=EcoRI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 2377..2382 /label=NcoI /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccga tctgcgcagc accatggcct gaaataacct 61 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt 121 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 181 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 241 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 301 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgaCTA ATTTTTTTTA 361 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 421 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC Ttgattcttc tgacacaaca gtctcgaact 481 taagctgcag aagttggtcg tgaggcactg ggcagGTAAG TATCAAGGTT ACAAGACAGG 541 TTTAAGGAGA CCAATAGAAA CTGGGCTTGT CGAGACAGAG AAGACTCTTG CGTTTCTGAT 601 AGGCACCTAT TGGTCTTACT GACATCCACT TTGCCTTTCT CTCCACAGgt gtccactccc 661 agttcaatta cagctcttaa ggctagagta ctTAATACGA CTCACTATAG Gctagccacc 721 atgacttcga aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg 781 tgggccagat gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa 841 aaacatgcag aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg 901 cgacatgttg tgccacatat tgagccagta gcgcggtgta ttataccaga ccttattggt 961 atgggcaaat caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat 1021 cttactgcat ggtttgaact tcttaattta ccaaagaaga tcatttttgt cggccatgat 1081 tggggtgctt gtttggcatt tcattatagc tatgagcatc aagataagat caaagcaata 1141 gttcacgctg aaagtgtagt agatgtgatt gaatcatggg atgaatggcc tgatattgaa 1201 gaagatattg cgttgatcaa atctgaagaa ggagaaaaaa tggttttgga gaataacttc 1261 ttcgtggaaa ccatgttgcc atcaaaaatc atgagaaagt tagaaccaga agaatttgca 1321 gcatatcttg aaccattcaa agagaaaggt gaagttcgtc gtccaacatt atcatggcct 1381 cgtgaaatcc cgttagtaaa aggtggtaaa cctgacgttg tacaaattgt taggaattat 1441 aatgcttatc tacgtgcaag tgatgattta ccaaaaatgt ttattgaatc ggacccagga 1501 ttcttttcca atgctattgt tgaaggtgcc aagaagtttc ctaatactga atttgtcaaa 1561 gtaaaaggtc ttcatttttc gcaagaagat gcacctgatg aaatgggaaa atatatcaaa 1621 tcgttcgttg agcgagttct caaaaatgaa caataattct aggggctcga gcgcccctct 1681 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 1741 gtctatatgt tattttccac catattgccg tcttttggca acgtgagggc ccggaaacct 1801 ggccctgtct tcttgacgag caatcctaga gcttcttgaa gacaaacaac gtctgtagcg 1861 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 1921 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 1981 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 2041 cagaaggtac ccccttgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 2101 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt 2161 tgaaaaacac gaattcAGCC AAACTCTCAA TTATCATAGA GGGAGAAACC AAAGTATTCC 2221 ACGACAAAAC CAAATTCACG CATTATCTCT CCACGAATCC AGCCCTTCAA AGGATAATAA 2281 CAGAAAAAAA CCAATACAAG AACGGGAACA ACGCCCTAGA AAAAACAAGA AGGTAATCCC 2341 TCAACAAACC TAAAAGAAGA CAGCCACAAG AACAGAccat ggaagacgcc aaaaacataa 2401 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata 2461 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg 2521 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga 2581 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 2641 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 2701 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg 2761 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa 2821 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 2881 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata 2941 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg 3001 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg 3061 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg 3121 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 3181 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg 3241 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta 3301 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg 3361 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag 3421 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 3481 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag 3541 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga 3601 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg 3661 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc 3721 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag 3781 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg 3841 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa 3901 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg 3961 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga 4021 tcgccgtgta attctagagt cggggcggcc ggccgcttcg agCAGACATG ATAAGATACA 4081 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA 4141 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA 4201 ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA 4261 AGTAAAACCT CTACAAATGT GGTAaaatcg ataaggatct gaaCGATGGA GCGGAGAATG 4321 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 4381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 4441 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 4501 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC ggatccgtcg accgatgccc 4561 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 4621 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc 4681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 5041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 5101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 5161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 5221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 5281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 5341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 5401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 5461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 5521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 5581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 5641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 5701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 5761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 5821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 5881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 5941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 6001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 6061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 6121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 6181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 6241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 6301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 6361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 6421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 6481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 6541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 6601 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 6841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 6901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 6961 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 7021 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt cgccattcag 7081 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa 7141 gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa 7201 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca 7261 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt 7321 gcaagtgcag gtgccagaac atttctctat cgata //