LOCUS HP_GAL_1073_1222_C 10730 bp ds-DNA circular 01-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID HP-GAL/1073-1222/CAT standard; circular DNA; ; 10730 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493821423| CC VNTDBDATE|493821423| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers 5'UTR 4597..4746 /vntifkey="52" /label=1073-1222 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 7156..7481 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 7517..8311 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green mutation 4686..4686 /vntifkey="62" /label=ATG-->ATA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 8486..8616 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink stem_loop 984..1039 /vntifkey="39" /label=Hairpin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6384..6615 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 1164..4307 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green primer_bind complement(6365..6382) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green primer_bind complement(8651..8671) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green CDS 1341..1409 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin complement(9039..9721) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 6695..7001 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 5502..6348 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6157..6348) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(8677..8699) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 9816..10676 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4753..5412 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccccggccgg ccggccggcc ggccaaaggc cggccggccg 1021 gccggccggc tagcggtacg gtaccgagct cggatccact agtaacggcc gccagtgtgc 1081 tggaattctg cagatatcca tcacactggc ggccgcaatt cccggggatc gaaagagcct 1141 gctaaagcaa aaaagaagtc accatgtcgt ttactttgac caacaagaac gtgattttcg 1201 ttgccggtct gggaggcatt ggtctggaca ccagcaagga gctgctcaag cgcgatcccg 1261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 1321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 1381 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg 1441 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa 1501 actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg 1561 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg 1621 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg 1681 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt 1741 ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc 1801 tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt 1861 tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca 1921 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg 1981 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca 2041 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg 2101 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct 2161 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg 2221 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt 2281 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg 2341 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct 2401 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg 2461 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg 2521 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta 2581 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg 2641 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg 2701 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag 2761 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag 2821 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt 2881 tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg 2941 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg 3001 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg 3061 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt 3121 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata 3181 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc 3241 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg 3301 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt 3361 cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga 3421 cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca 3481 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt 3541 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc 3601 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac 3661 gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata 3721 cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct 3781 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg 3841 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg 3901 cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc 3961 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt 4021 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac 4081 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg 4141 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt 4201 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattacagc 4261 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca 4321 ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa 4381 acttttggat gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc 4441 cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta 4501 tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg 4561 acaaactcgg cctcgactct aggcggccgc ctcgagtgat ttctttttgt ggtaaaaatc 4621 ttagttcatg tgaagaaatt tcatgtgaat gttttagcta tcaaacagta ctgtcaccta 4681 ctcatacaca aaactgcctc ccaaagactt ttcccaggtc cctcgtatca aaacattaag 4741 agtatactcg agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg 4801 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc 4861 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat 4921 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca 4981 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat 5041 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt 5101 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa 5161 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt 5221 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat 5281 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt 5341 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 5401 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct 5461 gaataagtga taataagcgg atgaatggca gaaattcgcc ggatctttgt gaaggaacct 5521 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta 5581 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt 5641 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa 5701 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa 5761 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa 5821 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt 5881 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta 5941 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac 6001 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta 6061 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat 6121 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 6181 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 6241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgcc tagagggccc 6361 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag 6421 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 6481 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 6541 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 6601 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 6661 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 6721 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 6781 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc 6841 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 6901 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 6961 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 7021 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct 7081 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 7141 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc 7201 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 7261 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 7321 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 7381 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 7441 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 7501 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 7561 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 7621 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 7681 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 7741 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 7801 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 7861 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 7921 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 7981 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 8041 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 8101 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 8161 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 8221 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 8281 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 8341 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 8401 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 8461 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa 8521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 8581 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc 8641 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 8701 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 8761 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 8821 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 8881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 8941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 9001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 9061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 9121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 9181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 9241 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 9301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 9361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 9421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 9481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 9541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 9601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 9661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 9721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 9781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 9841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 9901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 9961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 10021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 10081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 10141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 10201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 10261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 10321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 10381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 10441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 10501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 10561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 10621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 10681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag //