LOCUS GAL_1073_1222_CAT_ 10667 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/1073-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5439..6285 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6094..6285) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6321..6552 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6302..6319) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(8976..9658) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 9753..10613 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7093..7418 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(8588..8608) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7454..8248 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(8614..8636) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 6632..6938 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8423..8553 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..4683 /label=Hiap2_1073-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 4690..5349 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gagtgatttc tttttgtggt aaaaatctta 4561 gttcatgtga agaaatttca tgtgaatgtt ttagctatca aacagtactg tcacctactc 4621 atacacaaaa ctgcctccca aagacttttc ccaggtccct cgtatcaaaa cattaagagt 4681 atactcgaga tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat 4741 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt 4801 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 4861 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg 4921 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag 4981 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta 5041 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 5101 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 5161 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat 5221 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat 5281 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 5341 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa 5401 taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac 5461 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 5521 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 5581 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 5641 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 5701 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 5761 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 5821 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc 5881 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg 5941 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt 6001 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag 6061 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 6121 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 6181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6241 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgcctag agggccctat 6301 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 6361 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 6421 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 6481 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 6541 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 6601 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 6661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 6721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt 6781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 6841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 6901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 6961 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat 7021 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 7081 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 7141 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 7201 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 7261 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 7321 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 7381 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 7441 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 7501 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 7561 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 7621 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 7681 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 7741 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 7801 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 7861 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 7921 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 7981 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 8041 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 8101 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 8161 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 8221 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 8281 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 8341 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 8401 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 8461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 8521 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 8581 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 8641 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 8701 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 8761 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 8821 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 8881 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 8941 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 9001 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 9061 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 9121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 9181 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 9241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 9301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 9361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 9421 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 9481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 9541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 9601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 9661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 9721 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 9781 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 9841 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 9901 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 9961 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 10021 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 10081 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 10141 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 10201 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 10261 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 10321 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 10381 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 10441 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 10501 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 10561 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 10621 ctttttcaat attattgaag catttatcag ggttattgtc tcatgag //