LOCUS HIAP2_1073_1222_CA 7193 bp ds-DNA circular 03-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT ID 1073-1222/CAT\CUA1147 standard; circular DNA; ; 7193 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC VNTDATE|493819821| CC VNTDBDATE|493819821| CC LSOWNER| CC VNTAUTHORNAME|Demo User| FH Key Location/Qualifiers FH COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1965..2811 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink promoter 3619..3944 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin complement(5502..6184) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 3980..4774 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal 2847..3078 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(2828..2845) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4949..5079 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 6279..7139 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green polyA_signal complement(2620..2811) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(5114..5134) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 3158..3464 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_binding complement(5140..5162) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 1216..1875 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green misc_feature 1060..1209 /label=Hiap2_1073-1222 /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=green mutation 1136..1136 /vntifkey="62" /label=CTG-->CTA /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=pink misc_feature 1149..1149 /label=ATG-->ATA /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcctcgagt gatttctttt tgtggtaaaa 1081 atcttagttc atgtgaagaa atttcatgtg aatgttttag ctatcaaaca gtactatcac 1141 ctactcatac acaaaactgc ctcccaaaga cttttcccag gtccctcgta tcaaaacatt 1201 aagagtatac tcgagatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 1261 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 1321 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 1381 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg 1441 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 1501 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 1561 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 1621 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 1681 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 1741 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc 1801 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 1861 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg 1921 cctgaataag tgataataag cggatgaatg gcagaaattc gccggatctt tgtgaaggaa 1981 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag 2041 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta 2101 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag 2161 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct 2221 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca 2281 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct 2341 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct 2401 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca 2461 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt 2521 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat 2581 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 2641 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 2701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 2761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gcctagaggg 2821 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 2881 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 2941 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 3001 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 3061 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 3121 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3481 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 3541 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 3601 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 3661 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3721 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3841 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 3901 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 3961 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4021 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4081 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4141 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 4201 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4261 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4321 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4381 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4441 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 4501 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 4561 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 4621 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 4681 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4741 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4801 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4861 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4921 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 4981 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5041 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 5101 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5161 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5221 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5281 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 5341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5701 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 5761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5941 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 6001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 6841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 6901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 6961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gag //