LOCUS pCITE4a 3703 bp ds-DNA circular 20-DEC-2008 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers polyA_site 701..730 /vntifkey="26" /label=poly(A) sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 3687..3703 /vntifkey="30" /label=T7 promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 enhancer 16..518 /vntifkey="9" /label=CITE /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink protein_bind 534..578 /vntifkey="31" /label=S tag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink protein_bind 670..687 /vntifkey="31" /label=His tag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin complement(1007..1007) /vntifkey="33" /label=origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb CDS complement(1768..2625) /vntifkey="4" /label=bla (amp) sequence /ApEinfo_fwdcolor=#d3ff00 /ApEinfo_revcolor=#d3ff00 rep_origin complement(3064..3519) /vntifkey="33" /label=f1 origin /ApEinfo_fwdcolor=#00c0cb /ApEinfo_revcolor=#00c0cb misc_feature 1..1 /label=T7 promoter transcription start site (+1) /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff protein_bind 594..611 /vntifkey="31" /label=thrombin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 759..823 /label=3UTR of T7 minor capsid protein, between gene10B and gene11 /ApEinfo_fwdcolor=#abffff /ApEinfo_revcolor=#abffff misc_feature 17..516 /label=EMCV IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan terminator 806..806 /note="T7 transcription terminator Tphi" /label=T7 transcription terminator Tphi /ApEinfo_fwdcolor=#ff00cb /ApEinfo_revcolor=#ff00cb misc_feature 516..701 /label=S-tag N-terminal fusion protein /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 731..738 /label=PmeI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 739..744 /label=SpeI site /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 ORIGIN 1 gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 61 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc gtattcaaca 361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg 481 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc caccacccat atgaaagaaa 541 ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatcagggt accctggtgc 601 cacgcggttc catggctgat atcggatccg aattcgagct ccgtcgacaa gcttgcggcc 661 gcactcgagc accaccacca ccaccactga gatctgactg aaaaaaaaaa aaaaaaaaaa 721 aaaaaaaaaa gtttaaacac tagtccgctg agcaataact agcataaccc cttggggcct 781 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccggg cttcctcgct 841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1441 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 1801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 2041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 2101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 2161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 2221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 2281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 2401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 2461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 2521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 2761 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 2821 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 2881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 2941 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 3001 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 3061 aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 3121 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 3181 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 3241 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 3301 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 3361 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 3421 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 3481 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg ccattcaggc 3541 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 3601 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 3661 gttgtaaaac gacggccagt gaattgtaat acgactcact ata //