LOCUS       hGFP_IREScp_GUS_fi        2709 bp ds-DNA   linear       26-AUG-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          902..2709
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /strain="C24"
                     /db_xref="taxon:3702"
                     /chromosome="3"
                     /ecotype="C24"
                     /note="transgenic line M18"
                     /label=transgenic line M18
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    1..720
                     /label=sGFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    complement(896..901)
                     /label=NcoI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    complement(719..724)
                     /label=HindIII
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     CDS             902..2709
                     /gene="gus"
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /protein_id="CAC43289.1"
                     /db_xref="GI:14594807"
                     /db_xref="GOA:Q93VY4"
                     /db_xref="HSSP:P08236"
                     /db_xref="InterPro:IPR006101"
                     /db_xref="InterPro:IPR006102"
                     /db_xref="InterPro:IPR006103"
                     /db_xref="InterPro:IPR006104"
                     /db_xref="InterPro:IPR008979"
                     /db_xref="UniProtKB/TrEMBL:Q93VY4"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
                     /label=beta-glucuronidase
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    complement(737..742)
                     /label=SmaI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    complement(748..753)
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    898..2709
                     /label=GUS
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    complement(748..895)
                     /label=crTMV IRES_CP, 148bp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
ORIGIN
        1 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
       61 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
      121 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
      181 CTCGTGACCA CCTTCACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
      241 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
      301 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
      361 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
      421 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
      481 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
      541 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
      601 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
      661 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCACGGCA TGGACGAGCT GTACAAGTAA
      721 gcttatcgat ttcgaacccg gggtaccgaa ttcgtcgatt cggttgcagc atttaaagcg
      781 gttgacaact ttaaaagaag gaaaaagaag gttgaagaaa agggtgtagt aagtaagtat
      841 aagtacagac cggagaagta cgccggtcct gattcgttta atttgaaaga agaaaccATG
      901 GTCCGTCCTG TAGAAACCCC AACCCGTGAA ATCAAAAAAC TCGACGGCCT GTGGGCATTC
      961 AGTCTGGATC GCGAAAACTG TGGAATTGAT CAGCGTTGGT GGGAAAGCGC GTTACAAGAA
     1021 AGCCGGGCAA TTGCTGTGCC AGGCAGTTTT AACGATCAGT TCGCCGATGC AGATATTCGT
     1081 AATTATGCGG GCAACGTCTG GTATCAGCGC GAAGTCTTTA TACCGAAAGG TTGGGCAGGC
     1141 CAGCGTATCG TGCTGCGTTT CGATGCGGTC ACTCATTACG GCAAAGTGTG GGTCAATAAT
     1201 CAGGAAGTGA TGGAGCATCA GGGCGGCTAT ACGCCATTTG AAGCCGATGT CACGCCGTAT
     1261 GTTATTGCCG GGAAAAGTGT ACGTATCACC GTTTGTGTGA ACAACGAACT GAACTGGCAG
     1321 ACTATCCCGC CGGGAATGGT GATTACCGAC GAAAACGGCA AGAAAAAGCA GTCTTACTTC
     1381 CATGATTTCT TTAACTATGC CGGAATCCAT CGCAGCGTAA TGCTCTACAC CACGCCGAAC
     1441 ACCTGGGTGG ACGATATCAC CGTGGTGACG CATGTCGCGC AAGACTGTAA CCACGCGTCT
     1501 GTTGACTGGC AGGTGGTGGC CAATGGTGAT GTCAGCGTTG AACTGCGTGA TGCGGATCAA
     1561 CAGGTGGTTG CAACTGGACA AGGCACTAGC GGGACTTTGC AAGTGGTGAA TCCGCACCTC
     1621 TGGCAACCGG GTGAAGGTTA TCTCTATGAA CTGTGCGTCA CAGCCAAAAG CCAGACAGAG
     1681 TGTGATATCT ACCCGCTTCG CGTCGGCATC CGGTCAGTGG CAGTGAAGGG CGAACAGTTC
     1741 CTGATTAACC ACAAACCGTT CTACTTTACT GGCTTTGGTC GTCATGAAGA TGCGGACTTA
     1801 CGTGGCAAAG GATTCGATAA CGTGCTGATG GTGCACGACC ACGCATTAAT GGACTGGATT
     1861 GGGGCCAACT CCTACCGTAC CTCGCATTAC CCTTACGCTG AAGAGATGCT CGACTGGGCA
     1921 GATGAACATG GCATCGTGGT GATTGATGAA ACTGCTGCTG TCGGCTTTAA CCTCTCTTTA
     1981 GGCATTGGTT TCGAAGCGGG CAACAAGCCG AAAGAACTGT ACAGCGAAGA GGCAGTCAAC
     2041 GGGGAAACTC AGCAAGCGCA CTTACAGGCG ATTAAAGAGC TGATAGCGCG TGACAAAAAC
     2101 CACCCAAGCG TGGTGATGTG GAGTATTGCC AACGAACCGG ATACCCGTCC GCAAGGTGCA
     2161 CGGGAATATT TCGCGCCACT GGCGGAAGCA ACGCGTAAAC TCGACCCGAC GCGTCCGATC
     2221 ACCTGCGTCA ATGTAATGTT CTGCGACGCT CACACCGATA CCATCAGCGA TCTCTTTGAT
     2281 GTGCTGTGCC TGAACCGTTA TTACGGATGG TATGTCCAAA GCGGCGATTT GGAAACGGCA
     2341 GAGAAGGTAC TGGAAAAAGA ACTTCTGGCC TGGCAGGAGA AACTGCATCA GCCGATTATC
     2401 ATCACCGAAT ACGGCGTGGA TACGTTAGCC GGGCTGCACT CAATGTACAC CGACATGTGG
     2461 AGTGAAGAGT ATCAGTGTGC ATGGCTGGAT ATGTATCACC GCGTCTTTGA TCGCGTCAGC
     2521 GCCGTCGTCG GTGAACAGGT ATGGAATTTC GCCGATTTTG CGACCTCGCA AGGCATATTG
     2581 CGCGTTGGCG GTAACAAGAA AGGGATCTTC ACTCGCGACC GCAAACCGAA GTCGGCGGCT
     2641 TTTCTGCTGC AAAAACGCTG GACTGGCATG AACTTCGGTG AAAAACCGCA GCAGGGAGGC
     2701 AAACAATGA     
//