LOCUS pRst-Kv14_12-F 6739 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 698..1654 /label=RLuc /ApEinfo_fwdcolor=#00ff06 /ApEinfo_revcolor=#00ff06 misc_feature 337..409 /label=SV40_promoter_and_enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 11..253 /label=SV40_promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 669..688 /label=T7_promoter /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff misc_feature 687..687 /label=T7_transcription_start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 493..625 /label=chimeric_intron /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 4677..4898 /label=SV40_late_polyA /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 254..336 /label=SV40_promoter(1) /ApEinfo_label=SV40_promoter /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 325..374 /label=Putative_major_transcription_start_sites /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 1660..1696 /label=Multiple_cloning_site /ApEinfo_fwdcolor=#ff5b00 /ApEinfo_revcolor=#ff5b00 misc_feature 2989..2994 /label=NcoI /ApEinfo_fwdcolor=#e6b83e /ApEinfo_revcolor=#e6b83e misc_feature 1762..1767 /label=HindIII /ApEinfo_fwdcolor=#ff9b86 /ApEinfo_revcolor=#ff9b86 misc_feature 2760..2765 /label=XbaI /ApEinfo_fwdcolor=#ffb368 /ApEinfo_revcolor=#ffb368 misc_feature 2994..2996 /label=AfeI-cleaved-right_part /ApEinfo_fwdcolor=#ecc376 /ApEinfo_revcolor=#ecc376 misc_feature 410..421 /label=SV40_promoter_enhancer /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 1662..1767 /label=hairpin /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 1782..1801 /label=FWD-primer-Negulescu /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 3003..4655 /label=fluc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 4657..4662 /label=XbaI(1) /ApEinfo_label=XbaI /ApEinfo_fwdcolor=#e1d8db /ApEinfo_revcolor=#e1d8db misc_feature 1769..1773 /label=BglII_cleaved /ApEinfo_fwdcolor=#ee9e66 /ApEinfo_revcolor=#ee9e66 misc_feature 1656..1656 /label=BglII_cleaved(1) /ApEinfo_label=BglII_cleaved /ApEinfo_fwdcolor=#ec9c4b /ApEinfo_revcolor=#ec9c4b misc_feature 1657..1661 /label=BamHI-cleaved_right /ApEinfo_fwdcolor=#dba96b /ApEinfo_revcolor=#dba96b misc_feature 1768..1768 /label=BamHI-cleaved_left /ApEinfo_fwdcolor=#dba370 /ApEinfo_revcolor=#dba370 misc_feature 2997..3002 /label=BamHI /ApEinfo_fwdcolor=#d9c3be /ApEinfo_revcolor=#d9c3be misc_feature 1793..2990 /label=Kv1.4 /ApEinfo_fwdcolor=#c0ffff /ApEinfo_revcolor=#c0ffff misc_feature 2760..2990 /label=Kv1.4_0.2kb_IRES /ApEinfo_fwdcolor=#00d9ff /ApEinfo_revcolor=#00d9ff ORIGIN 1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt 61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga 481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg 541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac 601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc 661 tagagtacTT AATACGACTC ACTATAGGct agccaccatG acttcgaaag tttatgatcc 721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa 781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat 841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg 961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct 1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca 1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga 1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc 1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga 1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg 1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga 1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca 1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa 1621 aaatgaacaa atGtcgaTCG ACgggggccc ctaggAgatc cAAGCTTATC GATTTCGAAC 1681 CCGGGGTACC GAATTCCTCG AGTCTAGAGG AgcatgcTCC TCTAGACTCG AGGAATTCGG 1741 TACCCCGGGT TCGAAATCGA TAAGCTTgGA TCTAGCagct tAGCTAGAGA GCAAGACTGG 1801 GATGGATACT GTTTTAAGGA ATGCAGGCTT AACAAGTGAT CGCTGCTGTC TAGGATTTTG 1861 AGTCTTTTTC GGAGAACCTT GACTTCCGTT CCCAGCCCAT GTCTGCTGTG CCGAACTCCA 1921 GAGGAACCAG AAATCTCCGG GGTCTACCTT GGGGCGTCCC CAATCTCCAC CTCTGGGCTC 1981 CAGTAACGAG GACTCTGCAA TACCCCCTAG CCCCCTGGCC AAGACAACCG AACTTGTTCC 2041 GTGGATATTT GGGATCCTCC ACCTGCCAAA CCTGAGCGAT TTTTTTTGTA CTGCGCCCCC 2101 ACCCCCAATG ATTCTGCCCC TCCTCCAGCT GTTGCAGCGT GGAAAAGGGG AAACAAATCA 2161 CCGGGGGGGA TTTTTTTCGT CTATTTTTAT TTTTCGCACT TGCTGGGAAT GGTGAAGTGC 2221 TTCTTGTGAA TGATTCTAGC CAAAGGATGC TCTTCATTTC CTGCTTTCTA TGGAGACCTC 2281 AGTGTTGAGT TTGCCTCTGC TGGAACTGCG TCTACCACCT TCTCTACCTT CAAGGTCTTT 2341 GCCTCTATCT ACAACCTGGC ATTGCCTGTG GGTCCATGAA GGCTTGGATG ACCTTAGAAG 2401 GAAAGGTCTG GGAGTCCACC TTCATAGACT AAGCAGCAAT GGCTGGGCAT ATTTTAAGCC 2461 GCATTTTAAC ATGGGTCAAG CCATCAGTAG AAGGCAAGTG CTAAGACTAA AGACTTATTT 2521 GAATTTTATT TAAATTAGAT GGACTGGGCC TTGGCCAATT TCCATGCAAG AAAAAGTATA 2581 TTTCATTTTC TAGGCACAAC TTCTGAGTGT CAGATACTTG CTGTCTTTGA GTCTTGTGGC 2641 GTCATCACCG GACAGCATCC CAGACAGACT TCCAGATTTG AACATCTACC CCCCAACACG 2701 TAGGTGTATG GGAGACCACA TCATTTCATG ACTTATGTTT GAGGAACACT AGGCTGTTGT 2761 CTAGACGAGG CAAGCTCTGG AAAGCAACGC CGAGTCTCTG AGAAGAGGGA GCATAGGCTG 2821 TGCTGATTTA AAAACAGAAA ATGCAAAGTT GGACTGAAAA TATCCCACGT CTTCTAAGCA 2881 ATCTGCTTAA GGCTTCCAAA CTTACCTTAA TTTGGTAAGA AAATAAGCTG CCCTATTTTT 2941 CTTTCTTCTT CTCTTACAAC TGGAAGCAGC CATTTCCCCA AACCACCAcc atgGCTGGAT 3001 CCATGGaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg 3061 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa 3121 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt 3181 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg 3241 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag 3301 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca 3361 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc 3421 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg 3481 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg 3541 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg 3601 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct 3661 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg 3721 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat 3781 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt 3841 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc 3901 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct 3961 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat 4021 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg 4081 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga 4141 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg 4201 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt 4261 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt 4321 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc 4381 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac 4441 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg 4501 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg 4561 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca 4621 taaaggccaa gaagggcgga aagatcgccg tgtaatTCTA GAgcggccgc ttcgagcaga 4681 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 4741 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 4801 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 4861 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagt ttaaacagat 4921 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4981 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 5041 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 5101 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 5161 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 5221 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 5281 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 5341 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 5401 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 5461 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 5521 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 5581 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 5641 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 5701 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 5761 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 5821 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 5881 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 5941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 6241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6721 tttgctcaca tggctcgac //