LOCUS PBCRLucPFLuc 6988 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 2126..2753 /label=poliovirus_type1_Mahoney_IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 1148..2083 /label=RLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 2869..4521 /label=FLuc /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1032..1050 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3..795 /label=CMV immediate early promoter/enhancer /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 5968..6828 /label=beta-lactamase /ApEinfo_fwdcolor=#00ff59 /ApEinfo_revcolor=#00ff59 misc_feature 4627..4848 /label=SV40 late poly (A) signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 5194..5808 /label=replication region from pMB1 /ApEinfo_fwdcolor=#ff5600 /ApEinfo_revcolor=#ff5600 misc_feature 1049..1049 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 855..987 /label=chimeric intron /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan rep_origin 5209..5209 /note="pUC18 Ori; RNaseH cleavage point" /label=pUC18 Ori; RNaseH cleavage point /ApEinfo_fwdcolor=SlateGray3 /ApEinfo_revcolor=SlateGray3 misc_feature 734..734 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff66ff /ApEinfo_revcolor=#ff66ff ORIGIN 1 aaTATTGGCC ATTAGCCATA TTATTCATTG GTTATATAGC ATAAATCAAT ATTGGCTATT 61 GGCCATTGCA TACGTTGTAT CTATATCATA ATATGTACAT TTATATTGGC TCATGTCCAA 121 TATGACCGCC ATGTTGGCAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT 181 CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC 241 CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG 301 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 361 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTCCGCC CCCTATTGAC GTCAATGACG 421 GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ACGGGACTTT CCTACTTGGC 481 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACACCA 541 ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA 601 ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AATAACCCCG 661 CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC 721 GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC AGTTAAATTG 781 CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGA AGTTGGTCGT 841 GAGGCACTGG GCAGGTAAGT ATCAAGGTTA CAAGACAGGT TTAAGGAGAC CAATAGAAAC 901 TGGGCTTGTC GAGACAGAGA AGACTCTTGC GTTTCTGATA GGCACCTATT GGTCTTACTG 961 ACATCCACTT TGCCTTTCTC TCCACAGGTG TCCACTCCCA GTTCAATTAC AGCTCTTAAG 1021 GCTAGAGTAC TTAATACGAC TCACTATAGG ctagcctcga ggaactggaa aaccagaaag 1081 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggaattg gtttaaacga 1141 attcgaaATG ACTTCGAAAG TTTATGATCC AGAACAAAGG AAACGGATGA TAACTGGTCC 1201 GCAGTGGTGG GCCAGATGTA AACAAATGAA TGTTCTTGAT TCATTTATTA ATTATTATGA 1261 TTCAGAAAAA CATGCAGAAA ATGCTGTTAT TTTTTTACAT GGTAACGCGG CCTCTTCTTA 1321 TTTATGGCGA CATGTTGTGC CACATATTGA GCCAGTAGCG CGGTGTATTA TACCAGACCT 1381 TATTGGTATG GGCAAATCAG GCAAATCTGG TAATGGTTCT TATAGGTTAC TTGATCATTA 1441 CAAATATCTT ACTGCATGGT TTGAACTTCT TAATTTACCA AAGAAGATCA TTTTTGTCGG 1501 CCATGATTGG GGTGCTTGTT TGGCATTTCA TTATAGCTAT GAGCATCAAG ATAAGATCAA 1561 AGCAATAGTT CACGCTGAAA GTGTAGTAGA TGTGATTGAA TCATGGGATG AATGGCCTGA 1621 TATTGAAGAA GATATTGCGT TGATCAAATC TGAAGAAGGA GAAAAAATGG TTTTGGAGAA 1681 TAACTTCTTC GTGGAAACCA TGTTGCCATC AAAAATCATG AGAAAGTTAG AACCAGAAGA 1741 ATTTGCAGCA TATCTTGAAC CATTCAAAGA GAAAGGTGAA GTTCGTCGTC CAACATTATC 1801 ATGGCCTCGT GAAATCCCGT TAGTAAAAGG TGGTAAACCT GACGTTGTAC AAATTGTTAG 1861 GAATTATAAT GCTTATCTAC GTGCAAGTGA TGATTTACCA AAAATGTTTA TTGAATCGGA 1921 CCCAGGATTC TTTTCCAATG CTATTGTTGA AGGTGCCAAG AAGTTTCCTA ATACTGAATT 1981 TGTCAAAGTA AAAGGTCTTC ATTTTTCGCA AGAAGATGCA CCTGATGAAA TGGGAAAATA 2041 TATCAAATCG TTCGTTGAGC GAGTTCTCAA AAATGAACAA TAAgtcgacc tgcagaagct 2101 tgcgcagacg tcgcgcgcgg ccgttttaaa acagctctgg ggttgtaccc accccagagg 2161 cccacgtggc ggctagtact ccggtattgc ggtacccttg tacgcctgtt ttatactccc 2221 ttcccgtaac ttagacgcac aaaaccaagt tcaatagaag ggggtacaaa ccagtaccac 2281 cacgaacaag cacttctgtt tccccggtga tgtcgtatag actgcttgcg tggttgaaag 2341 cgacggatcc gttatccgct tatgtacttc gagaagccca gtaccacctc ggaatcttcg 2401 atgcgttgcg ctcagcactc aaccccagag tgtagcttag gctgatgagt ctggacatcc 2461 ctcaccggtg acggtggtcc aggctgcgtt ggcggcctac ctatggctaa cgccatggga 2521 cgctagttgt gaacaaggtg tgaagagcct attgagctac ataagaatcc tccggcccct 2581 gaatgcggct aatcccaacc tcggagcagg tggtcacaaa ccagtgattg gcctgtcgta 2641 acgcgcaagt ccgtggcgga accgactact ttgggtgtcc gtgtttcctt ttattttatt 2701 gtggctgctt atggtgacaa tcacagattg ttatcataaa gcgaattgga ttgcggcgcg 2761 ccgaattcga gctcgcccgg ggatcctcta gagtcagctt gaattccttt gtgttacatt 2821 cttgaatgtc gctcgcagtg acattagcat tccggtactg ttggtaaaAT GGAAGACGCC 2881 AAAAACATAA AGAAAGGCCC GGCGCCATTC TATCCTCTAG AGGATGGAAC CGCTGGAGAG 2941 CAACTGCATA AGGCTATGAA GAGATACGCC CTGGTTCCTG GAACAATTGC TTTTACAGAT 3001 GCACATATCG AGGTGAACAT CACGTACGCG GAATACTTCG AAATGTCCGT TCGGTTGGCA 3061 GAAGCTATGA AACGATATGG GCTGAATACA AATCACAGAA TCGTCGTATG CAGTGAAAAC 3121 TCTCTTCAAT TCTTTATGCC GGTGTTGGGC GCGTTATTTA TCGGAGTTGC AGTTGCGCCC 3181 GCGAACGACA TTTATAATGA ACGTGAATTG CTCAACAGTA TGAACATTTC GCAGCCTACC 3241 GTAGTGTTTG TTTCCAAAAA GGGGTTGCAA AAAATTTTGA ACGTGCAAAA AAAATTACCA 3301 ATAATCCAGA AAATTATTAT CATGGATTCT AAAACGGATT ACCAGGGATT TCAGTCGATG 3361 TACACGTTCG TCACATCTCA TCTACCTCCC GGTTTTAATG AATACGATTT TGTACCAGAG 3421 TCCTTTGATC GTGACAAAAC AATTGCACTG ATAATGAATT CCTCTGGATC TACTGGGTTA 3481 CCTAAGGGTG TGGCCCTTCC GCATAGAACT GCCTGCGTCA GATTCTCGCA TGCCAGAGAT 3541 CCTATTTTTG GCAATCAAAT CATTCCGGAT ACTGCGATTT TAAGTGTTGT TCCATTCCAT 3601 CACGGTTTTG GAATGTTTAC TACACTCGGA TATTTGATAT GTGGATTTCG AGTCGTCTTA 3661 ATGTATAGAT TTGAAGAAGA GCTGTTTTTA CGATCCCTTC AGGATTACAA AATTCAAAGT 3721 GCGTTGCTAG TACCAACCCT ATTTTCATTC TTCGCCAAAA GCACTCTGAT TGACAAATAC 3781 GATTTATCTA ATTTACACGA AATTGCTTCT GGGGGCGCAC CTCTTTCGAA AGAAGTCGGG 3841 GAAGCGGTTG CAAAACGCTT CCATCTTCCA GGGATACGAC AAGGATATGG GCTCACTGAG 3901 ACTACATCAG CTATTCTGAT TACACCCGAG GGGGATGATA AACCGGGCGC GGTCGGTAAA 3961 GTTGTTCCAT TTTTTGAAGC GAAGGTTGTG GATCTGGATA CCGGGAAAAC GCTGGGCGTT 4021 AATCAGAGAG GCGAATTATG TGTCAGAGGA CCTATGATTA TGTCCGGTTA TGTAAACAAT 4081 CCGGAAGCGA CCAACGCCTT GATTGACAAG GATGGATGGC TACATTCTGG AGACATAGCT 4141 TACTGGGACG AAGACGAACA CTTCTTCATA GTTGACCGCT TGAAGTCTTT AATTAAATAC 4201 AAAGGATATC AGGTGGCCCC CGCTGAATTG GAATCGATAT TGTTACAACA CCCCAACATC 4261 TTCGACGCGG GCGTGGCAGG TCTTCCCGAC GATGACGCCG GTGAACTTCC CGCCGCCGTT 4321 GTTGTTTTGG AGCACGGAAA GACGATGACG GAAAAAGAGA TCGTGGATTA CGTCGCCAGT 4381 CAAGTAACAA CCGCGAAAAA GTTGCGCGGA GGAGTTGTGT TTGTGGACGA AGTACCGAAA 4441 GGTCTTACCG GAAAACTCGA CGCAAGAAAA ATCAGAGAGA TCCTCATAAA GGCCAAGAAG 4501 GGCGGAAAGT CCAAATTGTA Aaatgtaact gtattcagcg atgacgaaat tcttagctat 4561 tgtaatagct gcaggcatgc aagcttgcgc agacgtcgcg cgcggccgct taagctagct 4621 tcgatcCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT 4681 GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA 4741 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG 4801 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG 4861 ATCCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGCC 4921 TTAATTAAGG GTTTAAACCC GGTACCCAGT CACGTAGCGA TAGCGGAGTG TACTGCATTA 4981 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 5041 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTtT TCCATAGGCT 5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 5641 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTga tcttttctac 5821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 5881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 5941 tatatatgag taaacttggt ctgacagTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 6001 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 6061 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 6121 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 6181 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 6241 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 6301 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 6361 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 6421 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 6481 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 6541 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 6601 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 6661 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 6721 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 6781 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATac tcttcctttt 6841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 6901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 6961 cgtcggtacc atttaaatat cgatcttc //