LOCUS pEGFP-CAT-NDST4-10 6010 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 8..589 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 583..583 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff19ff /ApEinfo_revcolor=#ff19ff misc_feature 1956..2675 /label=EGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 CDS 3906..4700 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=DarkOrange1 /ApEinfo_revcolor=DarkOrange1 misc_feature 2926..3381 /label=f1 single strand DNA origin /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3722..3857 /label=SV40 origin of replication /ApEinfo_fwdcolor=#eb3c34 /ApEinfo_revcolor=#eb3c34 misc_feature 3823..3823 /label=major transcription start /ApEinfo_fwdcolor=#ff41ff /ApEinfo_revcolor=#ff41ff misc_feature 3555..3626 /label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff2649 /ApEinfo_revcolor=#ff2649 misc_feature 3745..3765 /label=21-bp repeat /ApEinfo_fwdcolor=#ffab80 /ApEinfo_revcolor=#ffab80 misc_feature join(1375..1591,1775..1934) /label=NDST4-del_100_bp_at_3prime_end /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 1601..1607 /label=TATA box of the P2 promoter for short isoform /ApEinfo_fwdcolor=#00abff /ApEinfo_revcolor=#00abff misc_feature 2867..2879 /label=mRNA ends somewhere here /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3478..3478 /label=transcription start (+1) of Kan/Neo resistance /ApEinfo_fwdcolor=#ff4e54 /ApEinfo_revcolor=#ff4e54 misc_feature 3818..3818 /label=major transcription start(1) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff33ff /ApEinfo_revcolor=#ff33ff misc_feature 5285..5928 /label=pUC plasmid replication origin /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 3723..3743 /label=21-bp repeat(1) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff96a6 /ApEinfo_revcolor=#ff96a6 misc_feature 2829..2834 /label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3443..3448 /label=-35 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3774..3774 /label=major transcription start(2) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff21ff /ApEinfo_revcolor=#ff21ff misc_feature 4936..4941 /label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3627..3698 /label=enhancer (72-bp tandem repeat)(1) /ApEinfo_label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff5059 /ApEinfo_revcolor=#ff5059 misc_feature 2858..2863 /label=SV40 early polyA signal(1) /ApEinfo_label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3466..3471 /label=-10 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3812..3812 /label=major transcription start(3) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff36ff /ApEinfo_revcolor=#ff36ff misc_feature 4949..4954 /label=HSV polyA signal(1) /ApEinfo_label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3702..3722 /label=21-bp repeat(2) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff7890 /ApEinfo_revcolor=#ff7890 misc_feature 652..1311 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 1592..1595 /label=CAAT box of the P2 promoter for short isoform /ApEinfo_fwdcolor=#009cff /ApEinfo_revcolor=#009cff misc_feature 1652..1774 /label=region similar to calcium influx channel (htrp3) mR /ApEinfo_fwdcolor=#0088ff /ApEinfo_revcolor=#0088ff ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG 721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG 901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA 961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT 1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG 1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA 1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA 1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTGAAAAG 1381 ACCGACTCCA GCTAAGCAAG AGGCACAGCA GCAGTGGCAG AAGCAGACAG GAGCAGCTGG 1441 TATTCCGGTG ATTAAATAGT TCTCATAGCC TTTTTGCTGT ACCCGGTCTG CTCCCTGGGG 1501 TAAGGcTCGT GTCCGAGAAG GAAGCTGCAT TTTAACGGTG TGCTGAACAG AGGTCTGAAT 1561 CATTCCAGCT GGCTGCTTGT TTTGAAAAGG ACAATGTGTG TATAAATAAA AAGACTGCTG 1621 GAAGGGTCCA GACACCGGCA CCGGAGCTGA GGAGTGCTCT CAGAATCCTG TTGAGGCAGA 1681 TGGCACGAAC TGAAAGCTCC GCTAATTAAC GTGGAGCCAA GTAAACCTGA ATTCTGGATA 1741 TCTCATTTTC TAACTTCGGA TAAATTCAAG TTAGCAAAAG AAAAAAAAAT TGAAATGCTT 1801 CTCTAGATAA TGCTTAAAGG ACCCTGCAGC AGTTCTTGGT ATTGGACATA GCATCTCAGC 1861 TGTTGCCTCC TGGTCCGTTG ACTACCAGAT TTGACTCATT TGTGATTCCT TCGAGAATCT 1921 CCCATGATTT GCAAATGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT 1981 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA 2041 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT 2101 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG 2161 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA 2221 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 2281 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 2341 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC 2401 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC 2461 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 2521 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 2581 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 2641 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT AGATCATAAT 2701 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT 2761 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA 2821 TGGTTACAaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 2881 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 3361 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 3481 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 3541 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 3601 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 3661 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 3721 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3781 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 3841 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 3901 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3961 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 4021 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 4081 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 4141 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 4201 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 4261 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 4321 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4381 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 4441 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4501 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4561 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 4621 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 4681 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 4741 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 4801 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 4861 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 4921 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 4981 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 5041 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 5101 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 5161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 5221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 5281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 5341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 5401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 5461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 5521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 5581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 5641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 5701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 5761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 5821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 5881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 5941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 6001 cgccatgcat //