LOCUS pEGFP-CAT-NDST1_13 5868 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 8..589 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 583..583 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff19ff /ApEinfo_revcolor=#ff19ff misc_feature 1814..2533 /label=EGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 CDS 3764..4558 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=DarkOrange1 /ApEinfo_revcolor=DarkOrange1 misc_feature 2784..3239 /label=f1 single strand DNA origin /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3580..3715 /label=SV40 origin of replication /ApEinfo_fwdcolor=#eb3c34 /ApEinfo_revcolor=#eb3c34 misc_feature 3681..3681 /label=major transcription start /ApEinfo_fwdcolor=#ff41ff /ApEinfo_revcolor=#ff41ff misc_feature 3413..3484 /label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff2649 /ApEinfo_revcolor=#ff2649 misc_feature 3603..3623 /label=21-bp repeat /ApEinfo_fwdcolor=#ffab80 /ApEinfo_revcolor=#ffab80 misc_feature 1375..1795 /label=NDST1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 2725..2737 /label=mRNA ends somewhere here /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3336..3336 /label=transcription start (+1) of Kan/Neo resistance /ApEinfo_fwdcolor=#ff4e54 /ApEinfo_revcolor=#ff4e54 misc_feature 3676..3676 /label=major transcription start(1) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff33ff /ApEinfo_revcolor=#ff33ff misc_feature 5143..5786 /label=pUC plasmid replication origin /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 3581..3601 /label=21-bp repeat(1) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff96a6 /ApEinfo_revcolor=#ff96a6 misc_feature 2687..2692 /label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3301..3306 /label=-35 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3632..3632 /label=major transcription start(2) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff21ff /ApEinfo_revcolor=#ff21ff misc_feature 4794..4799 /label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3485..3556 /label=enhancer (72-bp tandem repeat)(1) /ApEinfo_label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff5059 /ApEinfo_revcolor=#ff5059 misc_feature 2716..2721 /label=SV40 early polyA signal(1) /ApEinfo_label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3324..3329 /label=-10 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3670..3670 /label=major transcription start(3) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff36ff /ApEinfo_revcolor=#ff36ff misc_feature 4807..4812 /label=HSV polyA signal(1) /ApEinfo_label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3560..3580 /label=21-bp repeat(2) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff7890 /ApEinfo_revcolor=#ff7890 misc_feature 652..1311 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG 721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG 901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA 961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT 1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG 1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA 1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA 1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTTGCTGG 1381 CGTCCCGGCC GAGGCAGCAT CCCAGCCTTT ACCCTGTGGG CCAACGTGAG GAGTGTGAGG 1441 AGCGGCATGC AGCGCCCCCT GGCTGGTTCG GTGTCTTTTG ACCTGCTGTT GACTGCTCCC 1501 AGGGGGCCCT GACGCCCTGG GGCACTTTTG CTCTGCACAG GACCACGCTG GGGTTTGCCA 1561 TGGTGACATA AAGGGGTGTA GAGGAGGGAA GGAGCAAGAC CAGCCTGTAG ACTGTGCCCC 1621 TGGCTGGGCA AAAGGGCCAG GGGCCCGGAG CCTTCCCTCC ACTGCCCTCG AACGCCTACT 1681 TCCTAAGTCT CTGCATTTCT CAGTCAGTGG ACAGCTTTTG GGACTCTTGT GTGAGGACTT 1741 GGGGGGACCA GGCCAGGCTG GGCCTCTGCT GATTGAGATC TTGGGATCCA GAATGGATCC 1801 ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT 1861 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG 1921 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT 1981 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC 2041 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA 2101 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA 2161 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA 2221 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA 2281 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG 2341 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT 2401 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG 2461 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA 2521 GCTGTACAAG TAAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG 2581 GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT 2641 GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAaata aagcaatagc 2701 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 2761 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 2821 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 2881 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 2941 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 3001 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 3061 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 3121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 3181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 3241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3361 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 3421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3481 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3541 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 3601 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 3661 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 3721 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3781 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3841 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3901 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 3961 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4021 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4081 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4141 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4201 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4261 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 4321 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4381 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4441 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4501 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4561 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4621 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4681 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 4741 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 4801 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 4861 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 4921 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 4981 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 5041 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5101 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5281 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 5341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 5821 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat //