LOCUS pEB_1-920 7739 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 794..812 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 811..811 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 3061..3864 /label=eGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 497..796 /label=left half of vaccinia thimidine kinase /ApEinfo_fwdcolor=#003bff /ApEinfo_revcolor=#003bff misc_feature 1..63 /label=N-terminus of vaccinia putative membrane protein /ApEinfo_fwdcolor=#00ff3c /ApEinfo_revcolor=#00ff3c misc_feature 20..481 /label=vaccinia virion protein VACWR093 /ApEinfo_fwdcolor=#00ff5e /ApEinfo_revcolor=#00ff5e misc_feature 3881..4004 /label=T7 transcription terminator Tphi /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 7325..7325 /label=oriC_from_pUC19 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3985..3985 /label=T7 transcription terminator Tphi site /ApEinfo_fwdcolor=#ff10ff /ApEinfo_revcolor=#ff10ff misc_feature 867..1418 /label=EMCV_R IRES since poly(C) to -7 (gataatATG missing) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 5706..6566 /label=beta lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green misc_feature 1425..2084 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 3835..3874 /label=right part of MCS since BamHI /ApEinfo_fwdcolor=#db6bff /ApEinfo_revcolor=#db6bff misc_feature 2100..3047 /label=ERBV IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan ORIGIN 1 AAGCTTTTGC GATCAATAAA TGGATCACAA CCAGTATCTC TTAACGATGT TCTTCGCAGA 61 TGATGATTCA TTTTTTAAGT ATTTGGCTAG TCAAGATGAT GAATCTTCAT TATCTGATAT 121 ATTGCAAATC ACTCAATATC TAGACTTTCT GTTATTATTA TTGATCCAAT CAAAAAATAA 181 ATTAGAAGCC GTGGGTCATT GTTATGAATC TCTTTCAGAG GAATACAGAC AATTGACAAA 241 ATTCACAGAC TTTCAAGATT TTAAAAAACT GTTTAACAAG GTCCCTATTG TTACAGATGG 301 AAGGGTCAAA CTTAATAAAG GATATTTGTT CGACTTTGTG ATTAGTTTGA TGCGATTCAA 361 AAAAGAATCC TCTCTAGCTA CCACCGCAAT AGATCCTGTT AGATACATAG ATCCTCGTCG 421 CAATATCGCA TTTTCTAACG TGATGGATAT ATTAAAGTCG AATAAAGTGA ACAATAATTA 481 ATTCTTTATT GTCATCATGA ACGGCGGACA TATTCAGTTG ATAATCGGCC CCATGTTTTC 541 AGGTAAAAGT ACAGAATTAA TTAGACGAGT TAGACGTTAT CAAATAGCTC AATATAAATG 601 CGTGACTATA AAATATTCTA ACGATAATAG ATACGGAACG GGACTATGGA CGCATGATAA 661 GAATAATTTT GAAGCATTGG AAGCAACTAA ACTATGTGAT GTCTTGGAAT CAATTACAGA 721 TTTCTCCGTG ATAGGTATCG ATGAAGGACA GTTCTTTCCA GACATTGTTG AATTGATCTC 781 GATCCCGCGA AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGCGGG 841 ATCAATTCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT 901 AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG 961 TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC 1021 TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT 1081 CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG 1141 ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC 1201 CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG 1261 TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG 1321 GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC 1381 GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATAA TACCATGGAG AAAAAAATCA 1441 CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC 1501 AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA 1561 AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC 1621 TGATGAATGC TCATCCGGAA CTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG 1681 ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT 1741 GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT 1801 GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT 1861 CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT 1921 TCTTCGCCCC CGTTTTCACG ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC 1981 CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA 2041 ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGC GCTACCGGTC 2101 TTTTGTAACT GAGGTTTTGA CATCTCATTG TTTTTGAGCC CCAACGTCTT GGTGGATGCT 2161 CAAAGAAGAT TACTCTTGAT GCTTGGCTCT CAGAAAGAAT GGCACCTCAA TGTTTTGAGA 2221 CCCGATTCTT TTGAGTCGAG ACCATTTCCC CTTCCCTGAA GATTGCTTTT CTTCCAACTA 2281 AACCCCTATT GATGTGTTGG TCGTTTGCCA ATCGGAGGGC GACAGGTCGT TTGCCAATCG 2341 GAGGGCGACA GGCACAGGTC GCTCCGAGTT CTAGTAGTGT GGGAACTTGT TACTACTGAT 2401 GAAACGAGGT AGTGACACTG ACTACCTGCG AACGAGGTCG GGGCCCTCCC TTCTTCCTTC 2461 ACCCAACTTT CACTTTTCGT TCCACTTTAG CAGGGGTCTT CTTTCTATCC CCCTGGCGGC 2521 ATTGGAACTA GCCGTCGCGT CTTAACGCGC AGCCCTGAAG GCCCCACACC TTGTGGATCT 2581 TGCCGTGGGT ATGTTTCTGG CATGTGTTTC TCAAGCCTGC AACCGAAGCC GAACAGCCAC 2641 ATGAACAGTT TGAGCGTGGT AGCGCTGTGT GAGTTGGCGG TGGATCCCCC TCGTGGTAAC 2701 ACGAGCCCCC GTGGCCAAAA GCCCAGTGTT TACAGCACCT CTCACATCCA GGACGACCCC 2761 ATCCTGGCGC TCACTCTTAG TAGTATGGCT TAGTACGCAT TAGGTGGTAA GCCGAGCTCT 2821 CCCTCGGCCT TGTTCTGAAT GCACACATGT CTAGGGGCTA AGGATGTCCT ACAGGTACCC 2881 GCACGTAACC TTCAGAGAGT GCGGATCTGA GTAGGAGACC GTGGTGCACT GCTTTACAGA 2941 TGCAGCCCCG GTTTAAAAAG CGTCTATGCC CCTACAGGGT AGCGGTGGGC CGCGCCCTTT 3001 CCTTTTAAAA CTACTTGTTC TATGGTGACA ATGGCAGGAA ACATGATACC GGTCGCCACC 3061 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGCCCA TCCTGGTCGA GCTGGACGGC 3121 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 3181 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 3241 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 3301 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 3361 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 3421 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 3481 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 3541 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 3601 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 3661 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 3721 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA 3781 CTCAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC 3841 CTGCAGCTCG AGAGGCCTAA TTAATTAAGT CGACGATCCG GCTGCTAACA AAGCCCGAAA 3901 GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 3961 TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT CGAGATCAAT 4021 TCTGTGAGCG TATGGCAAAC GAAGGAAAAA TAGTTATAGT AGCCGCACTC GATGGGACAT 4081 TTCAACGTAA ACCGTTTAAT AATATTTTGA ATCTTATTCC ATTATCTGAA ATGGTGGTAA 4141 AACTAACTGC TGTGTGTATG AAATGCTTTA AGGAGGCTTC CTTTTCTAAA CGATTGGGTG 4201 AGGAAACCGA GATAGAAATA ATAGGAGGTA ATGATATGTA TCAATCGGTG TGTAGAAAGT 4261 GTTACATCGA CTCATAATAT TATATTTTTT ATCTAAAAAA CTAAAAATAA ACATTGCTTA 4321 AATTTTAATA TAATACTTAA AAATGGATGT TGTGTCGTTA GATAAACCGT TTATGTATTT 4381 TGAGGAAATT GATAATGAGT TAGATTACGA ACCAGAAAGT GCAAATGAGG TCGCAAAAAA 4441 ACTGCCGTAT CAAGGACAGT TAAAACTATT ACTAGGAGAA TTATTTTTTC TTAGTAAGTT 4501 ACAGCGACAC GGTATATTAG ATGGTGCCAC CGTAGTGTAT ATAGGATCTG CTCCCGGTAC 4561 ACATATACGT TATTTGAGAG ATCATTTCTA TAATTTAGGA GTGATCATCA AATGGATGCT 4621 AATTGACGGC CGCCATCATG ATCCTATTTT AAATGGATTG CGTGATGTGA CTCTAGTGAC 4681 TCGGTTCGTT GATGAGGAAT ATCTACGATC CATCAAAAAA CAACTGCATC CTTCTAAGAT 4741 TATTTTAATT TCTGATGTGA GATCCAAACG AGGAGGAAAT GAACCTAGTA CGGCGGATTT 4801 ACTAAGTAAT TACGCTCTAC AAAATGTCAT GATTAGTATT TTAAACCCCG TGGCGTCTAG 4861 TCTTAAATGG AGATGCCCGT TTCCAGATCA ATGGATCAAG GACTTTTATA TCCCACACGG 4921 TAATAAAATG TTACAACCTT TTGCTCCTTC ATATTCAGGG CCGTCGTTTT ACAACGTCGT 4981 GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC 5041 AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG 5101 AATGGCGAAT GGCGCGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 5161 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 5221 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 5281 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 5341 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 5401 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5461 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5521 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTCCCAGGTG 5581 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 5641 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 5701 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 5761 TTCCTCTTTT TGCTCACCCA GAAAGCGTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 5821 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 5881 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 5941 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 6001 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 6061 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 6121 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATCTAACTC 6181 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 6241 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 6301 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 6361 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 6421 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 6481 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 6541 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 6601 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 6661 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 6721 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 6781 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 6841 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 6901 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 6961 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 7021 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 7081 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 7141 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 7201 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 7261 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 7321 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 7381 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT 7441 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG 7501 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA 7561 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA 7621 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT 7681 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCC //