The description of the protein encoded in this ORF: heat shock 70kDa protein 1A
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: no
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 244-2169
The sequence used for this IRESite (NM_005345.5) is longer by 27bp at the very 5'-end than the 5'-UTR shown in
the article in Figure 1.
Slightly conflicting annotation of the chromosomal region in DQ451402 shows that the 5'-UTR of our entry
(taken over from RefSeq) might be too long. Seemingly complete cDNAs in CR624878 and DA486842.
The promoter-less plasmid was created by destroying the SV40 promoter. Activity of both Renilla and firefly
luciferases was monitored (primary data were not published) and no promoter activity was concluded. RNase
protection was used to confirm that only bicistronic transcripts were produced (Fig. 3).
The IRES name: hsp70 Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.
The IRES absolute position (the range includes START and STOP codons or their equivalents): 51-243
How IRES boundaries were determined: experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):
Deletion of the 5' end to nt 23 had no effect on the IRES activity. The HSP70 IRES appeared about 5x stronger
than HRV IRES.