IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: hopefully_full-length_mRNA
The mRNA/+RNA description:
Putative in vivo transcript from SV40 promoter with the chimeric intron spliced out containing
renilla_luciferase - hairpin_from_multiple_cloning_site - firefly_luciferase and is terminated after the late
SV40 polyA signal.
The mRNA/+RNA sequence represented in the +DNA notation:
Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence: reverse_engineered_sequence_and_should_match_experiment_except_both_UTRs
The description of the protein encoded in this ORF: Firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1279-2931
A hairpin stemp loop is in the intercistronic region as is actually based on sequences from multiple cloning
site from pGEM-4z vector. Transcript is terminated at the XbaI site after the second cistron. The plasmid is
know to generate alternatively spliced mRNA products in cases of: Kv1.4/KCNA4, CVB3 at least (Jimenez et al.
(2005) Fig. 2C).