IRESite_Id:220 summary record describing characteristics of LEF1 IRES

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `220`	Version: `5`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš	Last change: `2009-09-01 20:51:39`

IRESite record type:

natural_transcript

The shape of the nucleic acid molecule translated: `linear`	The quality of the mRNA/+RNA sequence: `hopefully_full-length_mRNA`
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule: `LEF1`	The genetic origin of this natural mRNA/+RNA: `nuclear`

The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
19923451

Synonyms of the gene name:

Synonym: TCF1ALPHA

The mRNA/+RNA description:

Homo sapiens lymphoid enhancer-binding factor 1 (LEF1) putative 3.0kb mRNA derived from newly characterized
P3 promoter. Poly(A)-tail was dropped.

The mRNA/+RNA sequence represented in the +DNA notation:

GCGCGGGAGG AGGAGAAGCA GTGGGGAGGC GCAGCCGCTC AcCTGCGGGG CAGGGCGCGG AGGAGGGACC CGGTGcGcGC TCTCGGGCCG AGGAACCAGG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ACGcGCCCGG AGCCTCGCAC GCGGCCAAGC TCGGGCGTCC CCTCCCCTCG GCCGGGCGAA CTCAAGGGGC GCAGCTCTTT GCTTTGACAG AGCTGGCCGG 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CGGAGGCGTG CAGAGCGGCG AGCCGGCGAG CCAGGCTGAG AAACTCGACC CGGGAACAAA GAGGGGTCGG ACTGAGTGTG TGTGTCGGCT CGAGCTCCGG 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GAGAGGCATT TGCCCGAGGC CCCGCTGTGA CTCCCCGAGA CTCCGCAGTG CCCTCCACTG GGAGTCCCCG CGCTTGCCGG AAAAACTTTA TTCTTGGCAA 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ACTTCTCTTT CTCTTCCCCT CCTCCTCGGC CCCCATCTTC TGCTCCTCCT CCTTCTCTAG CAGATTAAAT GAGCCTCGAG AAGAAAAACC GAAGCGAAAG 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

GGAAGAAAAT AAGAAGATCT AAAACGGACA TCTCCAGCGT GGGTGGCTCC TTTTTCTTTT TCTTTTTTTC CCACCCTTCA GGAAGTGGAC GTTTCGTTAT 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

CTTCTGATCC TTGCACCTTC TTTTGGGGCA AACGGGGCCC TTCTGCCCAG ATCCCCTCTC TTTTCTCGGA AAACAAACTA CTAAGTCGGC ATCCGGGGTA 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ACTACAGTGG AGAGGGTTTC CGCGGAGACG CGCCGCCGGA CCCTCCTCTG CACTTTGGGG AGGCGTGCTC CCTCCAGAAC CGGCGTTCTC CGCGCGCAAA 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

TCCCGGCGAC GCGGGGTCGC GGGGTGGCCG CCGGGGCAGC CTCGTCTAGC GCGCGCCGCG CAGACGCCCC CGGAGTCGCC AGCTACCGCA GCCCTCGCCG 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CCCAGTGCCC TTCGGCCTCG GGGCGGGCGC CTGCGTCGGT CTCCGCGAAG CGGGAAAGCG CGGCGGCCGC CGGGATTCGG GCGCCGCGGC AGCTGCTCCG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

GCTGCCGGCC GGCGGCCCCG CGCTCGCCCG CCCCGCTTCC GCCCGCTGTC CTGCTGCACG AACCCTTCCA ACTCTCCTTT CCTCCCCCAC CCTTGAGTTA 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CCCCTCTGTC TTTCCTGCTG TTGCGCGGGT GCTCCCACAG CGGAGCGGAG ATTACAGAGC CGCCGGGATG CCCGCTGGAT CCATGCCCCA ACTCTCCGGA 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

GGAGGTGGCG GCGGCGGGGG GGACCCGGAA CTCTGCGCCA CGGACGAGAT GATCCCCTTC AAGGACGAGG GCGATCCTCA GAAGGAAAAG ATCTTCGCCG 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

AGATCAGTCA TCCCGAAGAG GAAGGCGATT TAGCTGACAT CAAGTCTTCC TTGGTGAACG AGTCTGAAAT CATCCCGGCC AGCAACGGAC ACGAGGTGGC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

CAGACAAGCA CAAACCTCTC AGGAGCCCTA CCACGACAAG GCCAGAGAAC ACCCCGATGA CGGAAAGCAT CCAGATGGAG GCCTCTACAA CAAGGGACCC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TCCTACTCGA GTTATTCCGG GTACATAATG ATGCCAAATA TGAATAACGA CCCATACATG TCAAATGGAT CTCTTTCTCC ACCCATCCCG AGAACATCAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

ATAAAGTGCC CGTGGTGCAG CCATCCCATG CGGTCCATCC TCTCACCCCC CTCATCACTT ACAGTGACGA GCACTTTTCT CCAGGATCAC ACCCGTCACA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CATCCCATCA GATGTCAACT CCAAACAAGG CATGTCCAGA CATCCTCCAG CTCCTGATAT CCCTACTTTT TATCCCTTGT CTCCGGGTGG TGTTGGACAG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ATCACCCCAC CTCTTGGCTG GCAAGGTCAG CCTGTATATC CCATCACGGG TGGATTCAGG CAACCCTACC CATCCTCACT GTCAGTCGAC ACTTCCATGT 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CCAGGTTTTC CCATCATATG ATTCCCGGTC CTCCTGGTCC CCACACAACT GGCATCCCTC ATCCAGCTAT TGTAACACCT CAGGTCAAAC AGGAACATCC 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CCACACTGAC AGTGACCTAA TGCACGTGAA GCCTCAGCAT GAACAGAGAA AGGAGCAGGA GCCAAAAAGA CCTCACATTA AGAAGCCTCT GAATGCTTTT 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

ATGTTATACA TGAAAGAAAT GAGAGCGAAT GTCGTTGCTG AGTGTACTCT AAAAGAAAGT GCAGCTATCA ACCAGATTCT TGGCAGAAGG TGGCATGCCC 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

TCTCCCGTGA AGAGCAGGCT AAATATTATG AATTAGCACG GAAAGAAAGA CAGCTACATA TGCAGCTTTA TCCAGGCTGG TCTGCAAGAG ACAATTATGG 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

TAAGAAAAAG AAGAGGAAGA GAGAGAAACT ACAGGAATCT GCATCAGGTA CAGGTCCAAG AATGACAGCT GCCTACATCT GAAACATGGT GGAAAACGAA 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

GCTCATTCCC AACGTGCAAA GCCAAGGCAG CGACCCCAGG ACCTCTTCTG GAGATGGAAG CTTGTTGAAA ACCCAGACTG TCTCCACGGC CTGCCCAGTC 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

GACGCCAAAG GAACACTGAC ATCAATTTTA CCCTGAGGTC ACTGCTAGAG ACGCTGATCC ATAAAGACAA TCACTGCCAA CCCCTCTTTC GTCTACTGCA 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

AGAGCCAAGT TCCAAAATAA AGCATAAAAA GGTTTTTTAA AAGGAAATGT AAAAGCACAT GAGAATGCTA GCAGGCTGTG GGGCAGCTGA GCAGCTTTTC 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TCCCCCCATA TCTGCGTGCA CTTCCCAGAG CATCTTGCAT CCAAACCTGT AACCTTTCGG CAAGGACGGT AACTTGGCTG CATTTGCCTG TCATGCGCAA 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

CTGGAGCCAG CAACCAGCTA TCCATCAGCA CCCCAGTGGA GGAGTTCATG GAAGAGTTCC CTCTTTGTTT CTGCTTCATT TTTCTTTCTT TTCTTTTCTC 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

CTAAAGCTTT TATTTAACAG TGCAAAAGGA TCGTTTTTTT TTGCTTTTTT AAACTTGAAT TTTTTTAATT TACACTTTTT AGTTTTAATT TTCTTGTATA 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

TTTTGCTAGC TATGAGCTTT TAAATAAAAT TGAAAGTTCT GGAAAAGTTT GAAATAATGA CATAAAAAGA AGCCTTCTTT TTCTGAGACA GCTTGTCTGG 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

TAAGTGGCTT CTCTGTGAAT TGCCTGTAAC ACATAGTGGC TTCTCCGCCC TTGTAAGGTG TTCAGTAGAG CTAAATAAAT GTAATAGCCA AACCCCACTC 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

TGTTGGTAGC AATTGGCAGC CCTATTTCAG TTTATTTTTT CTTCTGTTTT CTTCTTTTCT TTTTTTAAAC AGTAAACCTT AACAGATGCG TTCAGCAGAC 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

TGGTTTGCAG TGAATTTTCA TTTCTTTCCT TATCACCCCC TTGTTGTAAA AAGCCCAGCA CTTGAATTGT TATTACTTTA AATGTTCTGT ATTTGTATCT 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

GTTTTTATTA GCCAATTAGT GGGATTTTAT GCCAGTTGTT AAAATGAGCA TTGATGTACC CATTTTTTAA AAAAGCAAGC ACAGCCTTTG CCCAAAACTG 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

TCATCCTAAC GTTTGTCATT CCAGTTTGAG TTAATGTGCT GAGCATTTTT TTAAAAGAAG CTTTGTAATA AAACATTTTT AAAAATTGTC ATTT
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591

Credibility of mRNA sequence:

reverse_engineered_fragment_and_the_rest_is_a_guess

The organism containing this mRNA with IRES segment in its genome:

Homo sapiens

A promoter reported in cDNA corresponding to IRES sequence:
yes The total number of notable open-reading frames (ORFs):
1

Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:

Summary of experiments studying integrity of the in vivo transcripts in a particular host:

Integrity (uniformity) of mRNA tested using Northern-blot:
unclear_result

Integrity (uniformity) of mRNA tested using RNase protection:
not_tested

Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested

Integrity (uniformity) of mRNA tested using primer extension :
not_tested

Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested

Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested

Integrity (uniformity) of mRNA tested using RNAi:
not_tested

Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested

Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
weak_promoter_confirmed

Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested

Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested

The organism used:

Cercopithecus aethiops COS-1 (ATCC CRL-1650)

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LEF1

The description of the protein encoded in this ORF:
lymphoid enhancer binding factor-1

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1168-2382

Remarks:

Authentic RNA pol II promoter was confirmed between bases +280 and +640 relative to the main start site of
transcription. Comment from J. Jimenez: Our original studies of the LEF1 5'UTR were based on characterizing
a third promoter in the 5'UTR that is responsible for generating a 3.0 kb transcript. Because we had evidence
of an authentic promoter, we performed N. blot analysis. We did also attempt RT-PCR, but had technical
problems due to the high GC content of the 5'UTR.

Possibility of aberrant splicing of the plasmids with LEF-1 was ruled out only by the Northern-blot technique
(Fig. 2C and 3B).


See also DNA record GI:22858703 covering the promoter region, 5'-UTR and short part of CDS:

     misc_feature    1371..1374
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="transcription initiation site 1"
     misc_feature    1506..1507
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="transcription initiation site 2"
     misc_feature    1821..1931
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Wnt response element (WRE)"
     misc_feature    2201..2202
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="transcription initiation site 3"
     misc_feature    2325..2326
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="transcription initiation site 4"
     mRNA            <2701..>2760
                     /product="LEF1"




One mRNA record and 3 EST records confirm that transcription initiation start site is within the first 99bp of
the 5'-UTR we present here:


>dbj|AK225772.1| UniGene infoGene info Homo sapiens mRNA for lymphoid enhancer binding factor-1 variant,
clone: FCC133A10
Length=2300

 Score = 3042 bits (1647),  Expect = 0.0
 Identities = 1707/1731 (98%), Gaps = 23/1731 (1%)
 Strand=Plus/Plus

Query  99    GGACGCGCCCGGAGCCTCGCACGCGGCCAAGCTC-GGGCGTCCCCTCCCCTCGGCCGGGC  157
             |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct  1     GGACGCGCCCGGAGCCTCGCACGCGGCCAAGCTCGGGGCGTCCCCTCCCCTCGGCCGGGC  60

[cut]


>gb|BM919836.1| UniGene info AGENCOURT_6708394 NIH_MGC_120 Homo sapiens cDNA clone IMAGE:5749600
5', mRNA sequence.
Length=1005

 Score = 1568 bits (849),  Expect = 0.0
 Identities = 934/970 (96%), Gaps = 29/970 (2%)
 Strand=Plus/Plus

Query  26   GAGGCGCAGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCC-GG-TGCGCGCTCT  83
            |||||||||||||||||||||||||||||||||||||||||||||| || ||||||||||
Sbjct  1    GAGGCGCAGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCCGGGCTGCGCGCTCT  60

[cut]



>gb|BQ215285.1| UniGene infoGeo AGENCOURT_7553707 NIH_MGC_92 Homo sapiens cDNA clone IMAGE:6045994
5', mRNA sequence.
Length=874

 Score = 1474 bits (798),  Expect = 0.0
 Identities = 851/873 (97%), Gaps = 17/873 (1%)
 Strand=Plus/Plus

Query  33   AGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCC-GG-TGCGCGCTCTCGGGCCG  90
            ||||||||||||||||||||||||||||||||||||||| || |||||||||||||||||
Sbjct  1    AGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCCGGGCTGCGCGCTCTCGGGCCG  60

[cut]


>emb|CR981946.1| UniGene info CR981946 RZPD no.9016 Homo sapiens cDNA clone RZPDp9016C0812
5', mRNA sequence.
Length=678

 Score = 1149 bits (622),  Expect = 0.0
 Identities = 660/678 (97%), Gaps = 10/678 (1%)
 Strand=Plus/Plus

Query  28   GGCGCAGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCC-GG-TGCGCGCTCTCG  85
            |||||||||||||||||||||||||||||||||||||||||||| || ||||||||||||
Sbjct  1    GGCGCAGCCGCTCACCTGCGGGGCAGGGCGCGGAGGAGGGACCCGGGCTGCGCGCTCTCG  60

[cut]

Citations:

Jimenez J., Jang G. M., Semler B. L., Waterman M. L. (2005) An internal ribosome entry site mediates translation of lymphoid enhancer factor-1. RNA. 11(9):1385-1399

IRESs:

IRES:

Version: `2`	Last change: `2007-01-24 00:00:00`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `LEF1`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

1-1167

Conclusion:
weakly_supported_IRES

How IRES boundaries were determined:
experimentally_determined

The sequence of IRES region aligned to its secondary structure (if available):

GCGCGGGAGG AGGAGAAGCA GTGGGGAGGC GCAGCCGCTC AcCTGCGGGG CAGGGCGCGG AGGAGGGACC CGGTGcGcGC TCTCGGGCCG AGGAACCAGG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ACGcGCCCGG AGCCTCGCAC GCGGCCAAGC TCGGGCGTCC CCTCCCCTCG GCCGGGCGAA CTCAAGGGGC GCAGCTCTTT GCTTTGACAG AGCTGGCCGG 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CGGAGGCGTG CAGAGCGGCG AGCCGGCGAG CCAGGCTGAG AAACTCGACC CGGGAACAAA GAGGGGTCGG ACTGAGTGTG TGTGTCGGCT CGAGCTCCGG 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GAGAGGCATT TGCCCGAGGC CCCGCTGTGA CTCCCCGAGA CTCCGCAGTG CCCTCCACTG GGAGTCCCCG CGCTTGCCGG AAAAACTTTA TTCTTGGCAA 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ACTTCTCTTT CTCTTCCCCT CCTCCTCGGC CCCCATCTTC TGCTCCTCCT CCTTCTCTAG CAGATTAAAT GAGCCTCGAG AAGAAAAACC GAAGCGAAAG 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

GGAAGAAAAT AAGAAGATCT AAAACGGACA TCTCCAGCGT GGGTGGCTCC TTTTTCTTTT TCTTTTTTTC CCACCCTTCA GGAAGTGGAC GTTTCGTTAT 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

CTTCTGATCC TTGCACCTTC TTTTGGGGCA AACGGGGCCC TTCTGCCCAG ATCCCCTCTC TTTTCTCGGA AAACAAACTA CTAAGTCGGC ATCCGGGGTA 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ACTACAGTGG AGAGGGTTTC CGCGGAGACG CGCCGCCGGA CCCTCCTCTG CACTTTGGGG AGGCGTGCTC CCTCCAGAAC CGGCGTTCTC CGCGCGCAAA 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

TCCCGGCGAC GCGGGGTCGC GGGGTGGCCG CCGGGGCAGC CTCGTCTAGC GCGCGCCGCG CAGACGCCCC CGGAGTCGCC AGCTACCGCA GCCCTCGCCG 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CCCAGTGCCC TTCGGCCTCG GGGCGGGCGC CTGCGTCGGT CTCCGCGAAG CGGGAAAGCG CGGCGGCCGC CGGGATTCGG GCGCCGCGGC AGCTGCTCCG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

GCTGCCGGCC GGCGGCCCCG CGCTCGCCCG CCCCGCTTCC GCCCGCTGTC CTGCTGCACG AACCCTTCCA ACTCTCCTTT CCTCCCCCAC CCTTGAGTTA 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CCCCTCTGTC TTTCCTGCTG TTGCGCGGGT GCTCCCACAG CGGAGCGGAG ATTACAGAGC CGCCGGG
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161

Remarks:

The IRES activity observed after direct RNA transfection was very low when compared to CVB3 IRES and did not
increase when PV 2A protease mRNA was cotransfected.

Citations:

Jimenez J., Jang G. M., Semler B. L., Waterman M. L. (2005) An internal ribosome entry site mediates translation of lymphoid enhancer factor-1. RNA. 11(9):1385-1399

Last change to the database: 2019-03-18 09:32:49 GMT+1