IRESite_Id:259 summary record describing characteristics of c-IAP1

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `259`	Version: `13`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš	Last change: `2009-09-01 21:34:55`

IRESite record type:

natural_transcript

The shape of the nucleic acid molecule translated: `linear`	The quality of the mRNA/+RNA sequence: `hopefully_full-length_mRNA`
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule: `c-IAP1`	The genetic origin of this natural mRNA/+RNA: `nuclear`

The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
41349435

Synonyms of the gene name:

Synonym: API1

Synonym: MIHB

Synonym: HIAP2

Synonym: RNF48

Synonym: cIAP1

Synonym: Hiap-2

Synonym: c-IAP1

Synonym: BIRC2

The mRNA/+RNA description:

Homo sapiens baculoviral IAP repeat-containing 2 (BIRC2), mRNA. Poly(A)-tail was dropped from the sequence.

The mRNA/+RNA sequence represented in the +DNA notation:

GGATGGGGCG GCGGGCTTCG GGAGCGCCCG GGCTGATCCG AGCCGAGCGG GCCGTATCTC CTTGTCGGCG CCGCTGATTC CCGGCTCTGC GGAGGCCTCT 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

AGGCAGCCGC GCAGCTTCCG TGTTTGCTGC GCCCGCACTG CGATTTACAA CCCTGAAGAA TCTCCCTATC CCTATTTTGT CCCCCTGCAG TAATAAATCC 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CATTATGGAG ATCTCGAAAC TTTATAAAGG GATATAGTTT GAATTCTATG GAGTGTAATT TTGTGTATGA ATTATATTTT TAAAACATTG AAGAGTTTTC 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

AGAAAGAAGG CTAGTAGAGT TGATTACTGA TACTTTATGC TAAGCAGTAC TTTTTTGGTA GTACAATATT TTGTTAGGCG TTTCTGATAA CACTAGAAAG 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

GACAAGTTTT ATCTTGTGAT AAATTGATTA ATGTTTACAA CATGACTGAT AATTATAGCT GAATAGTCCT TAAATGATGA ACAGGTTATT TAGTTTTTAA 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

ATGCAGTGTA AAAAGTGTGC TGTGGAAATT TTATGGCTAA CTAAGTTTAT GGAGAAAATA CCTTCAGTTG ATCAAGAATA ATAGTGGTAT ACAAAGTTAG 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

GAAGAAAGTC AACATGATGC TGCAGGAAAT GGAAACAAAT ACAAATGATA TTTAACAAAG ATAGAGTTTA CAGTTTTTGA ACTTTAAGCC AAATTCATTT 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

GACATCAAGC ACTATAGCAG GCACAGGTTC AACAAAGCTT GTGGGTATTG ACTTCCCCCA AAAGTTGTCA GCTGAAGTAA TTTAGCCCAC TTAAGTAAAT 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ACTATGATGA TAAGCTGTGT GAACTTAGCT TTTAAATAGT GTGACCATAT GAAGGTTTTA ATTACTTTTG TTTATTGGAA TAAAATGAGA TTTTTTGGGT 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

TGTCATGTTA AAGTGCTTAT AGGGAAAGAA GCCTGCATAT AATTTTTTAC CTTGTGGCAT AATCAGTAAT TGGTCTGTTA TTCAGGCTTC ATAGCTTGTA 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

ACCAAATATA AATAAAAGGC ATAATTTAGG TATTCTATAG TTGCTTAGAA TTTTGTTAAT ATAAATCTCT GTGAAAAATC AAGGAGTTTT AATATTTTCA 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

GAAGTGCATC CACCTTTCAG GGCTTTAAGT TAGTATTACT CAAGATTATG AACAAATAGC ACTTAGGTTA CCTGAAAGAG TTACTACAAC CCCAAAGAGT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

TGTGTTCTAA GTAGTATCTT GGTAATTCAG AGAGATACTC ATCCTACCTG AATATAAACT GAGATAAATC CAGTAAAGAA AGTGTAGTAA ATTCTACATA 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

AGAGTCTATC ATTGATTTCT TTTTGTGGTA AAAATCTTAG TTCATGTGAA GAAATTTCAT GTGAATGTTT TAGCTATCAA ACAGTACTGT CACCTACTCA 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

TGCACAAAAC TGCCTCCCAA AGACTTTTCC CAGGTCCCTC GTATCAAAAC ATTAAGAGTA TAATGGAAGA TAGCACGATC TTGTCAGATT GGACAAACAG 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

CAACAAACAA AAAATGAAGT ATGACTTTTC CTGTGAACTC TACAGAATGT CTACATATTC AACTTTCCCC GCCGGGGTGC CTGTCTCAGA AAGGAGTCTT 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

GCTCGTGCTG GTTTTTATTA TACTGGTGTG AATGACAAGG TCAAATGCTT CTGTTGTGGC CTGATGCTGG ATAACTGGAA ACTAGGAGAC AGTCCTATTC 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

AAAAGCATAA ACAGCTATAT CCTAGCTGTA GCTTTATTCA GAATCTGGTT TCAGCTAGTC TGGGATCCAC CTCTAAGAAT ACGTCTCCAA TGAGAAACAG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

TTTTGCACAT TCATTATCTC CCACCTTGGA ACATAGTAGC TTGTTCAGTG GTTCTTACTC CAGCCTTTCT CCAAACCCTC TTAATTCTAG AGCAGTTGAA 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

GACATCTCTT CATCGAGGAC TAACCCCTAC AGTTATGCAA TGAGTACTGA AGAAGCCAGA TTTCTTACCT ACCATATGTG GCCATTAACT TTTTTGTCAC 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CATCAGAATT GGCAAGAGCT GGTTTTTATT ATATAGGACC TGGAGATAGG GTAGCCTGCT TTGCCTGTGG TGGGAAGCTC AGTAACTGGG AACCAAAGGA 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TGATGCTATG TCAGAACACC GGAGGCATTT TCCCAACTGT CCATTTTTGG AAAATTCTCT AGAAACTCTG AGGTTTAGCA TTTCAAATCT GAGCATGCAG 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

ACACATGCAG CTCGAATGAG AACATTTATG TACTGGCCAT CTAGTGTTCC AGTTCAGCCT GAGCAGCTTG CAAGTGCTGG TTTTTATTAT GTGGGTCGCA 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ATGATGATGT CAAATGCTTT TGTTGTGATG GTGGCTTGAG GTGTTGGGAA TCTGGAGATG ATCCATGGGT AGAACATGCC AAGTGGTTTC CAAGGTGTGA 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

GTTCTTGATA CGAATGAAAG GCCAAGAGTT TGTTGATGAG ATTCAAGGTA GATATCCTCA TCTTCTTGAA CAGCTGTTGT CAACTTCAGA TACCACTGGA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

GAAGAAAATG CTGACCCACC AATTATTCAT TTTGGACCTG GAGAAAGTTC TTCAGAAGAT GCTGTCATGA TGAATACACC TGTGGTTAAA TCTGCCTTGG 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

AAATGGGCTT TAATAGAGAC CTGGTGAAAC AAACAGTTCA AAGTAAAATC CTGACAACTG GAGAGAACTA TAAAACAGTT AATGATATTG TGTCAGCACT 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TCTTAATGCT GAAGATGAAA AAAGAGAAGA GGAGAAGGAA AAACAAGCTG AAGAAATGGC ATCAGATGAT TTGTCATTAA TTCGGAAGAA CAGAATGGCT 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

CTCTTTCAAC AATTGACATG TGTGCTTCCT ATCCTGGATA ATCTTTTAAA GGCCAATGTA ATTAATAAAC AGGAACATGA TATTATTAAA CAAAAAACAC 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

AGATACCTTT ACAAGCGAGA GAACTGATTG ATACCATTTT GGTTAAAGGA AATGCTGCGG CCAACATCTT CAAAAACTGT CTAAAAGAAA TTGACTCTAC 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

ATTGTATAAG AACTTATTTG TGGATAAGAA TATGAAGTAT ATTCCAACAG AAGATGTTTC AGGTCTGTCA CTGGAAGAAC AATTGAGGAG GTTGCAAGAA 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

GAACGAACTT GTAAAGTGTG TATGGACAAA GAAGTTTCTG TTGTATTTAT TCCTTGTGGT CATCTGGTAG TATGCCAGGA ATGTGCCCCT TCTCTAAGAA 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

AATGCCCTAT TTGCAGGGGT ATAATCAAGG GTACTGTTCG TACATTTCTC TCTTAAAGAA AAATAGTCTA TATTTTAACC TGCATAAAAA GGTCTTTAAA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

ATATTGTTGA ACACTTGAAG CCATCTAAAG TAAAAAGGGA ATTATGAGTT TTTCAATTAG TAACATTCAT GTTCTAGTCT GCTTTGGTAC TAATAATCTT 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

GTTTCTGAAA AGATGGTATC ATATATTTAA TCTTAATCTG TTTATTTACA AGGGAAGATT TATGTTTGGT GAACTATATT AGTATGTATG TGTACCTAAG 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

GGAGTAGTGT CACTGCTTGT TATGCATCAT TTCAGGAGTT ACTGGATTTG TTGTTCTTTC AGAAAGCTTT GAATACTAAA TTATAGTGTA GAAAAGAACT 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

GGAAACCAGG AACTCTGGAG TTCATCAGAG TTATGGTGCC GAATTGTCTT TGGTGCTTTT CACTTGTGTT TTAAAATAAG GATTTTTCTC TTATTTCTCC 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

CCCTAGTTTG TGAGAAACAT CTCAATAAAG TGCTTTAAAA AG
^3701      ^3711      ^3721      ^3731      ^3741

Credibility of mRNA sequence:

only_fragment_published_or_from_author_and_the_rest_is_a_guess

The organism containing this mRNA with IRES segment in its genome:

Homo sapiens

A promoter reported in cDNA corresponding to IRES sequence:
no The total number of notable open-reading frames (ORFs):
1

Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:

Summary of experiments studying integrity of the in vivo transcripts in a particular host:

Integrity (uniformity) of mRNA tested using Northern-blot:
homogeneous_population_of_molecules_confirmed

Integrity (uniformity) of mRNA tested using RNase protection:
not_tested

Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested

Integrity (uniformity) of mRNA tested using primer extension :
not_tested

Integrity (uniformity) of mRNA tested using RT-PCR:
heterogeneous_population_of_molecules_found

Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested

Integrity (uniformity) of mRNA tested using RNAi:
not_tested

Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested

Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
no_promoter_confirmed

Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested

Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested

The organism used:

Homo sapiens HepG2 (ATCC HB-8065)

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
BIRC2

The description of the protein encoded in this ORF:
baculoviral IAP repeat-containing protein 2

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1400-3256

OPTIONAL: The function of the encoded protein

NFR2-TRAF signalling complex protein; apoptosis inhibitor 1

Remarks:

The cloned 5'-UTR by van Eden et al. (2004) region was not a complete 5'-UTR. There are 3 mutations in
the cloned sequence provided by R. Lloyd in comparison to both German and NIH cDNA projects: GI:34367137 and
GI:22382083. -297nt from initiator ATG is A instead of G, -177nt is A instead of T, -76nt is G instead of T.
The sequence provided to IRESite by R. Lloyd spans -1115 to +4 (while the +4 base not shown in van Eden
et al. (2004) Fig. 1A is 'G'. van Eden showed already published 5'-UTR of NM_001166.2 (GI:10880127) GenBank
record which is 1159b long whereas current,, updated record GI:41349435 (NM_001166.3) contains 1.4kb
untranslated region. Thus, the sequence presented here in IRESite is longer by 240 nt at the very 5'-end
compared to the sequence shown by van Eden in Figure 1.

It appears the ATG codons at positions 1400 and 1463 are in-frame. The TAA stop codon at postion 1453 of the
previously reported uORF (Lewis et al., (2005), Fig. 2) is not in-frame with the ATG codon and thus it appears
based on the current sequence that the uORF does not exist. As a consequence, the IRES reported by M. Holcik
group spans into the CDS region whereas the region studied by R. Lloyd group was within the 5'-UTR. BLASTN
search for ESTs returns matches which show the current sequence with no uORF is correct.

Aberrantly spliced mRNAs were detected by RT-PCR (van Eden et al. (2004) Fig. 4D) which were not detectable
by Northern blot. The splice junction was AAGAGAAAG/CTATCAAAC and the splice event removed part of the RLuc
and most of cIAP1 5'-UTR from the intercistronic region (only 26 bp remained immediately upstream of the
initiatior ATG codon). Similarly, monocistronic mRNAs containing the chimeric intron and the cIAP1 5'-UTR
spliced at the same position.

Possible presence of a cryptic promoter was studied in a promoter-less plasmid and only very weak FLuc
activity was detected (Fig. 4C). In contrast, the control pGL3-Basic vector also lacking an SV40 promoter
expressed much lower amount of FLuc activity.

IRES activity was confirmed by direct bicistronic mRNA transfection (Fig. 5) which was capped and
poly(A)-tailed.

Citations:

Van Eden M. E., Byrd M. P., Sherrill K. W., Lloyd R. E. (2004) Translation of cellular inhibitor of apoptosis protein 1 (c-IAP1) mRNA is IRES mediated and regulated during cell stress. RNA. 10(3):469-481

IRESs:

IRES:

Version: `5`	Last change: `2008-06-27 21:36:06`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `c-IAP1_285-1399`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

285-1399

Conclusion:
weakly_supported_IRES

How IRES boundaries were determined:
experimentally_determined

The sequence of IRES region aligned to its secondary structure (if available):

ACATTGAAGA GTTTTCAGAA AGAAGGCTAG TAGAGTTGAT TACTGATACT TTATGCTAAG CAGTACTTTT TTGGTAGTAC AATATTTTGT TAGGCGTTTC 
^285       ^295       ^305       ^315       ^325       ^335       ^345       ^355       ^365       ^375       

TGATAACACT AGAAAGGACA AGTTTTATCT TGTGATAAAT TGATTAATGT TTACAACATG ACTGATAATT ATAGCTGAAT AGTCCTTAAA TGATGAACAG 
^385       ^395       ^405       ^415       ^425       ^435       ^445       ^455       ^465       ^475       

GTTATTTAGT TTTTAAATGC AGTGTAAAAA GTGTGCTGTG GAAATTTTAT GGCTAACTAA GTTTATGGAG AAAATACCTT CAGTTGATCA AGAATAATAG 
^485       ^495       ^505       ^515       ^525       ^535       ^545       ^555       ^565       ^575       

TGGTATACAA AGTTAGGAAG AAAGTCAACA TGATGCTGCA GGAAATGGAA ACAAATACAA ATGATATTTA ACAAAGATAG AGTTTACAGT TTTTGAACTT 
^585       ^595       ^605       ^615       ^625       ^635       ^645       ^655       ^665       ^675       

TAAGCCAAAT TCATTTGACA TCAAGCACTA TAGCAGGCAC AGGTTCAACA AAGCTTGTGG GTATTGACTT CCCCCAAAAG TTGTCAGCTG AAGTAATTTA 
^685       ^695       ^705       ^715       ^725       ^735       ^745       ^755       ^765       ^775       

GCCCACTTAA GTAAATACTA TGATGATAAG CTGTGTGAAC TTAGCTTTTA AATAGTGTGA CCATATGAAG GTTTTAATTA CTTTTGTTTA TTGGAATAAA 
^785       ^795       ^805       ^815       ^825       ^835       ^845       ^855       ^865       ^875       

ATGAGATTTT TTGGGTTGTC ATGTTAAAGT GCTTATAGGG AAAGAAGCCT GCATATAATT TTTTACCTTG TGGCATAATC AGTAATTGGT CTGTTATTCA 
^885       ^895       ^905       ^915       ^925       ^935       ^945       ^955       ^965       ^975       

GGCTTCATAG CTTGTAACCA AATATAAATA AAAGGCATAA TTTAGGTATT CTATAGTTGC TTAGAATTTT GTTAATATAA ATCTCTGTGA AAAATCAAGG 
^985       ^995       ^1005      ^1015      ^1025      ^1035      ^1045      ^1055      ^1065      ^1075      

AGTTTTAATA TTTTCAGAAG TGCATCCACC TTTCAGGGCT TTAAGTTAGT ATTACTCAAG ATTATGAACA AATAGCACTT AGGTTACCTG AAAGAGTTAC 
^1085      ^1095      ^1105      ^1115      ^1125      ^1135      ^1145      ^1155      ^1165      ^1175      

TACAACCCCA AAGAGTTGTG TTCTAAGTAG TATCTTGGTA ATTCAGAGAG ATACTCATCC TACCTGAATA TAAACTGAGA TAAATCCAGT AAAGAAAGTG 
^1185      ^1195      ^1205      ^1215      ^1225      ^1235      ^1245      ^1255      ^1265      ^1275      

TAGTAAATTC TACATAAGAG TCTATCATTG ATTTCTTTTT GTGGTAAAAA TCTTAGTTCA TGTGAAGAAA TTTCATGTGA ATGTTTTAGC TATCAAACAG 
^1285      ^1295      ^1305      ^1315      ^1325      ^1335      ^1345      ^1355      ^1365      ^1375      

TACTGTCACC TACTC
^1385      ^1395

Remarks:

IRES activity measurable only in cells directly transfected by RNA was at the best comparable to the activity
of HCV IRES and about 2x the activity of negative control (capped and poly(A)-tailed RNAs). More importantly,
the Fluc/RLuc ratio values were around 0.08 so the IRES-driven expression was very low compared to the
cap-dependent initiation.

Citations:

IRES:

Version: `2`	Last change: `2008-06-27 21:12:29`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `c-IAP1_1313-1462`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

1313-1462

Conclusion:
weakly_supported_IRES

How IRES boundaries were determined:
experimentally_determined

The sequence of IRES region aligned to its secondary structure (if available):

TGATTTCTTT TTGTGGTAAA AATCTTAGTT CATGTGAAGA AATTTCATGT GAATGTTTTA GCTATCAAAC AGTACTGTCA CCTACTCATG CACAAAACTG 
^1313      ^1323      ^1333      ^1343      ^1353      ^1363      ^1373      ^1383      ^1393      ^1403      

CCTCCCAAAG ACTTTTCCCA GGTCCCTCGT ATCAAAACAT TAAGAGTATA
^1413      ^1423      ^1433      ^1443      ^1453

Remarks:

The minimal IRES as determined by Warnakulasuriyarachchi et al. (2004).

Citations:

Warnakulasuriyarachchi D., Cerquozzi S., Cheung H. H., Holcik M. (2004) Translational induction of the inhibitor of apoptosis protein HIAP2 during endoplasmic reticulum stress attenuates cell death and is mediated via an inducible internal ribosome entry site element. J. Biol. Chem. 279(17):17148-17157

IRES trans-acting factor (ITAFS):

IRES trans-acting factor (ITAF):

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

Type of the interaction between ITAF and the RNA subject to translation:
indirect_interaction

ITAF protein characteristics:

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

ITAF abbreviated name:
p97/DAP5/NAT1

ITAF fullname:
p97/DAP5/NAT1

ITAF description (long):
p97/DAP5/NAT1 cleavage product p86 oe eIF4G, 86 kDa, RNA binding protein

3.1.2. Organisms or in vitro systems where this ITAF was functionally studied:

Organism or in vitro system where ITAF was shown:

Necessity of ITAF for translation in this particular organism or system:
stimulatory

Method used to demonstrate ITAF effect:
in_vivo

The organism where action of this ITAF was studied:

Homo sapiens HEK 293T/17 (ATCC CRL-11268)

Remarks:

In contrast, the p86 cleavage product of p97/DAP5/NAT1 does not affect XIAP IRES.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1