IRESite record type: plasmid_with_promoter_and_putative_IRES_translationally_characterized
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: end-to-end_full-length_mRNA
The mRNA/+RNA description:
In vitro T3 run-off transcript containing 5' UTR of human p97 (DAP5, nt from -306 to -1) mRNA cloned upstream
of the luciferase reporter RNA bearing a poly(A) tail.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_completely_same_as_in_the_experiment
The name of the promoter used to express this mRNA: T3
Aliases of the plasmid name:
Alias: SL200PLA
Alias: SL200PL(A)
Alias: pSL200PLA
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing 5' UTR of human p97 (DAP5, nt from -306 to -1) and firefly luciferase coding region (luc
version of the luciferase gene) with the poly(A)tract.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: p97/DAP5
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
Plasmid sequence verified (partially/completely) by IRESite (more details in Remarks): plasmid sequence confirmed by IRESite curators by restriction analysis + parts by PCR
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
The total number of notable open-reading frames (ORFs): 1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence: