The nucleic acid data:
IRESite Id: 385 Version: 1
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš Last change: 2008-06-12 15:26:09
IRESite record type:
The shape of the nucleic acid molecule translated:
The quality of the mRNA/+RNA sequence:
The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
The mRNA/+RNA description: 
One of the two putative in vivo transcripts of mammalian expression vector pNBio: this one is from SV40
promoter only to the end of CDS because the SV40 late poly(A) signal is inserted in reverse direction and
therefore is probably non-functional.
The mRNA/+RNA sequence represented in the +DNA notation:

Credibility of mRNA sequence:
The name of the plasmid:
The name of the promoter used to express this mRNA:
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):
  not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing:
  not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
The description of the protein encoded in this ORF:
puromycin N-acetyltransferase
The translational frameshift (ribosome slippage) involved:
The ribosome read-through involved:
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
Kulman J. D., Satake M., Harris J. E. (2007) A versatile system for site-specific enzymatic biotinylation and regulated expression of proteins in cultured mammalian cells. Protein Expr. Purif. 52(2):320-328
Last change to the database: 2019-03-18 09:32:49 GMT+1